Prevalence of antibiotic resistance genes in mining-impacted farmland environments
Mining activities produce large quantities of tailings and acid mine drainage, which contain varieties of heavy metals, thereby affecting the downstream farmland soils and crops. Heavy metals could induce antibiotic resistance through co-selection pressure. However, the profiles of antibiotic resist...
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| Format: | Article |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324017275 |
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| author | Hai-Yan Zou Fang-Zhou Gao Liang-Ying He Min Zhang You-Sheng Liu Jun Qi Guang-Guo Ying |
| author_facet | Hai-Yan Zou Fang-Zhou Gao Liang-Ying He Min Zhang You-Sheng Liu Jun Qi Guang-Guo Ying |
| author_sort | Hai-Yan Zou |
| collection | DOAJ |
| description | Mining activities produce large quantities of tailings and acid mine drainage, which contain varieties of heavy metals, thereby affecting the downstream farmland soils and crops. Heavy metals could induce antibiotic resistance through co-selection pressure. However, the profiles of antibiotic resistance genes (ARGs) in the mining-affected farmland soils and crops are still unclear. Here we investigated contents of heavy metals, ARG abundances, mobile genetic elements (MGEs), and microbial community in mining-affected farmland soils and vegetables from Shangba village (SB), in comparison to a nearby reference village Taiping (TP). Results showed that in SB group, except for Cr, other metals were all above the Chinese Standards. When compared with the reference group, higher ARG abundances were detected in mining-affected farmland soils and vegetables, with great proportions of genes resistant to sulfonamides, chloramphenicols and tetracyclines. In addition, positive correlations were found between the above three ARG classes and heavy metals concentrations (especially Cu, Pb and Zn). Spearman’s correlations revealed that there were positive correlations between sul1 and total nitrogen, as well as tetB/P and pH. Additionally, the Shannon index values were different for the samples from two villages (p < 0.05). Proteobacteria and Actinobacteria were dominant phyla in soil samples. Network analysis suggested that multiple genera (belonging to Proteobacteria and Actinobacteria) were positively associated with many ARGs (p < 0.05), implying they might be potential hosts for ARGs. To sum up, this study provided clear evidence that mining activities caused severe heavy metals pollution to the farmland, thus posing co-selection pressure on the persistence of ARGs in the affected farmland environments. |
| format | Article |
| id | doaj-art-96b3e3799f74462d8218e97b2dd8e03c |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-96b3e3799f74462d8218e97b2dd8e03c2025-01-23T05:25:58ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117651Prevalence of antibiotic resistance genes in mining-impacted farmland environmentsHai-Yan Zou0Fang-Zhou Gao1Liang-Ying He2Min Zhang3You-Sheng Liu4Jun Qi5Guang-Guo Ying6SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, Guangzhou 510006, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, Guangzhou 510006, ChinaPearl River Water Resources Research Institute, Pearl River Water Resources Commission of the Ministry of Water Resources, Guangzhou, ChinaSCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; School of Environment, South China Normal University, Guangzhou 510006, ChinaSchool of Environment, South China Normal University, Guangzhou 510006, China; Corresponding authors.SCNU Environmental Research Institute, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety & MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou 510006, China; Corresponding authors.Mining activities produce large quantities of tailings and acid mine drainage, which contain varieties of heavy metals, thereby affecting the downstream farmland soils and crops. Heavy metals could induce antibiotic resistance through co-selection pressure. However, the profiles of antibiotic resistance genes (ARGs) in the mining-affected farmland soils and crops are still unclear. Here we investigated contents of heavy metals, ARG abundances, mobile genetic elements (MGEs), and microbial community in mining-affected farmland soils and vegetables from Shangba village (SB), in comparison to a nearby reference village Taiping (TP). Results showed that in SB group, except for Cr, other metals were all above the Chinese Standards. When compared with the reference group, higher ARG abundances were detected in mining-affected farmland soils and vegetables, with great proportions of genes resistant to sulfonamides, chloramphenicols and tetracyclines. In addition, positive correlations were found between the above three ARG classes and heavy metals concentrations (especially Cu, Pb and Zn). Spearman’s correlations revealed that there were positive correlations between sul1 and total nitrogen, as well as tetB/P and pH. Additionally, the Shannon index values were different for the samples from two villages (p < 0.05). Proteobacteria and Actinobacteria were dominant phyla in soil samples. Network analysis suggested that multiple genera (belonging to Proteobacteria and Actinobacteria) were positively associated with many ARGs (p < 0.05), implying they might be potential hosts for ARGs. To sum up, this study provided clear evidence that mining activities caused severe heavy metals pollution to the farmland, thus posing co-selection pressure on the persistence of ARGs in the affected farmland environments.http://www.sciencedirect.com/science/article/pii/S0147651324017275Antibiotic resistance genes (ARGs)MiningHeavy metalsMicrobial communityFarmland soilsVegetables |
| spellingShingle | Hai-Yan Zou Fang-Zhou Gao Liang-Ying He Min Zhang You-Sheng Liu Jun Qi Guang-Guo Ying Prevalence of antibiotic resistance genes in mining-impacted farmland environments Ecotoxicology and Environmental Safety Antibiotic resistance genes (ARGs) Mining Heavy metals Microbial community Farmland soils Vegetables |
| title | Prevalence of antibiotic resistance genes in mining-impacted farmland environments |
| title_full | Prevalence of antibiotic resistance genes in mining-impacted farmland environments |
| title_fullStr | Prevalence of antibiotic resistance genes in mining-impacted farmland environments |
| title_full_unstemmed | Prevalence of antibiotic resistance genes in mining-impacted farmland environments |
| title_short | Prevalence of antibiotic resistance genes in mining-impacted farmland environments |
| title_sort | prevalence of antibiotic resistance genes in mining impacted farmland environments |
| topic | Antibiotic resistance genes (ARGs) Mining Heavy metals Microbial community Farmland soils Vegetables |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324017275 |
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