Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection
This work describes a label-free electrochemical immunosensor for the sensing of Epstein-Barr virus (EBV) with high sensitivity. First, a monolayer of 1,6-hexanedithiol (HDT) was fabricated on the screen-printed electrode surface by the interaction between sulfur atoms and SPE. AuNPs can be modified...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/5711384 |
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| _version_ | 1832545869539311616 |
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| author | Miao Yu Ming Liu Yuan Li |
| author_facet | Miao Yu Ming Liu Yuan Li |
| author_sort | Miao Yu |
| collection | DOAJ |
| description | This work describes a label-free electrochemical immunosensor for the sensing of Epstein-Barr virus (EBV) with high sensitivity. First, a monolayer of 1,6-hexanedithiol (HDT) was fabricated on the screen-printed electrode surface by the interaction between sulfur atoms and SPE. AuNPs can be modified on the electrode by the Au-S bond formed between the HDT-free group and Au atom in AuNPs. Protein A is then adsorbed onto AuNPs. Several parameters were optimized. The optimum concentration of protein A is 0.6 mg/mL. The optimum immobilization time for protein A is 90 min. The optimum concentration of antibody is 80 μg/mL. The optimum immobilization time for antibody is 90 min. Directional immobilization of EBV antibody is achieved by high affinity binding of protein A to the Fc segment of antibody. When antigen specifically binds to antibody, the formation of immune complexes blocks electron transfer of [Fe(CN)6]4-/3- and is reflected in the detection of cyclic voltammetry/electrochemical impedance spectroscopy. The detection range is 1 pg/mL–l00 ng/mL with a LOD of 0.1 pg/mL. In addition, the proposed sensor exhibited an excellent antiinterference property. |
| format | Article |
| id | doaj-art-96afdcf7b0714d44a51eff4f44b18a25 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-96afdcf7b0714d44a51eff4f44b18a252025-02-03T07:24:27ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/5711384Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus DetectionMiao Yu0Ming Liu1Yuan Li2Department of OtorhinolaryngologyLogistics Support DepartmentDepartment of OphthalmologyThis work describes a label-free electrochemical immunosensor for the sensing of Epstein-Barr virus (EBV) with high sensitivity. First, a monolayer of 1,6-hexanedithiol (HDT) was fabricated on the screen-printed electrode surface by the interaction between sulfur atoms and SPE. AuNPs can be modified on the electrode by the Au-S bond formed between the HDT-free group and Au atom in AuNPs. Protein A is then adsorbed onto AuNPs. Several parameters were optimized. The optimum concentration of protein A is 0.6 mg/mL. The optimum immobilization time for protein A is 90 min. The optimum concentration of antibody is 80 μg/mL. The optimum immobilization time for antibody is 90 min. Directional immobilization of EBV antibody is achieved by high affinity binding of protein A to the Fc segment of antibody. When antigen specifically binds to antibody, the formation of immune complexes blocks electron transfer of [Fe(CN)6]4-/3- and is reflected in the detection of cyclic voltammetry/electrochemical impedance spectroscopy. The detection range is 1 pg/mL–l00 ng/mL with a LOD of 0.1 pg/mL. In addition, the proposed sensor exhibited an excellent antiinterference property.http://dx.doi.org/10.1155/2022/5711384 |
| spellingShingle | Miao Yu Ming Liu Yuan Li Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection Journal of Analytical Methods in Chemistry |
| title | Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection |
| title_full | Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection |
| title_fullStr | Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection |
| title_full_unstemmed | Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection |
| title_short | Point-of-Care Based Electrochemical Immunoassay for Epstein-Barr Virus Detection |
| title_sort | point of care based electrochemical immunoassay for epstein barr virus detection |
| url | http://dx.doi.org/10.1155/2022/5711384 |
| work_keys_str_mv | AT miaoyu pointofcarebasedelectrochemicalimmunoassayforepsteinbarrvirusdetection AT mingliu pointofcarebasedelectrochemicalimmunoassayforepsteinbarrvirusdetection AT yuanli pointofcarebasedelectrochemicalimmunoassayforepsteinbarrvirusdetection |