Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses

Summary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for h...

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Main Authors: Karim Abu Nahia, Cecilia Lanny Winata
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007512
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author Karim Abu Nahia
Cecilia Lanny Winata
author_facet Karim Abu Nahia
Cecilia Lanny Winata
author_sort Karim Abu Nahia
collection DOAJ
description Summary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms.For details on the use and execution of this protocol, please refer to Abu Nahia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
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institution Kabale University
issn 2666-1667
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publishDate 2025-03-01
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spelling doaj-art-96a4c6c29e144084ad9247b80114dbcd2025-01-20T04:17:51ZengElsevierSTAR Protocols2666-16672025-03-0161103586Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analysesKarim Abu Nahia0Cecilia Lanny Winata1International Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland; Corresponding authorInternational Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland; Corresponding authorSummary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms.For details on the use and execution of this protocol, please refer to Abu Nahia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007512Cell BiologySingle cellDevelopmental biologyGenomics
spellingShingle Karim Abu Nahia
Cecilia Lanny Winata
Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
STAR Protocols
Cell Biology
Single cell
Developmental biology
Genomics
title Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
title_full Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
title_fullStr Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
title_full_unstemmed Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
title_short Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
title_sort protocol for the preparation of zebrafish whole heart cell suspension for single cell analyses
topic Cell Biology
Single cell
Developmental biology
Genomics
url http://www.sciencedirect.com/science/article/pii/S2666166724007512
work_keys_str_mv AT karimabunahia protocolforthepreparationofzebrafishwholeheartcellsuspensionforsinglecellanalyses
AT cecilialannywinata protocolforthepreparationofzebrafishwholeheartcellsuspensionforsinglecellanalyses