Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
Summary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for h...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007512 |
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| _version_ | 1832594155625250816 |
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| author | Karim Abu Nahia Cecilia Lanny Winata |
| author_facet | Karim Abu Nahia Cecilia Lanny Winata |
| author_sort | Karim Abu Nahia |
| collection | DOAJ |
| description | Summary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms.For details on the use and execution of this protocol, please refer to Abu Nahia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-96a4c6c29e144084ad9247b80114dbcd |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-96a4c6c29e144084ad9247b80114dbcd2025-01-20T04:17:51ZengElsevierSTAR Protocols2666-16672025-03-0161103586Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analysesKarim Abu Nahia0Cecilia Lanny Winata1International Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland; Corresponding authorInternational Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland; Corresponding authorSummary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms.For details on the use and execution of this protocol, please refer to Abu Nahia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007512Cell BiologySingle cellDevelopmental biologyGenomics |
| spellingShingle | Karim Abu Nahia Cecilia Lanny Winata Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses STAR Protocols Cell Biology Single cell Developmental biology Genomics |
| title | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
| title_full | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
| title_fullStr | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
| title_full_unstemmed | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
| title_short | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
| title_sort | protocol for the preparation of zebrafish whole heart cell suspension for single cell analyses |
| topic | Cell Biology Single cell Developmental biology Genomics |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007512 |
| work_keys_str_mv | AT karimabunahia protocolforthepreparationofzebrafishwholeheartcellsuspensionforsinglecellanalyses AT cecilialannywinata protocolforthepreparationofzebrafishwholeheartcellsuspensionforsinglecellanalyses |