Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses
Summary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for h...
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Format: | Article |
Language: | English |
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Elsevier
2025-03-01
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Series: | STAR Protocols |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007512 |
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author | Karim Abu Nahia Cecilia Lanny Winata |
author_facet | Karim Abu Nahia Cecilia Lanny Winata |
author_sort | Karim Abu Nahia |
collection | DOAJ |
description | Summary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms.For details on the use and execution of this protocol, please refer to Abu Nahia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
format | Article |
id | doaj-art-96a4c6c29e144084ad9247b80114dbcd |
institution | Kabale University |
issn | 2666-1667 |
language | English |
publishDate | 2025-03-01 |
publisher | Elsevier |
record_format | Article |
series | STAR Protocols |
spelling | doaj-art-96a4c6c29e144084ad9247b80114dbcd2025-01-20T04:17:51ZengElsevierSTAR Protocols2666-16672025-03-0161103586Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analysesKarim Abu Nahia0Cecilia Lanny Winata1International Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland; Corresponding authorInternational Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland; Corresponding authorSummary: Obtaining viable cell suspension that accurately represents the diversity of complex tissues is challenging due to the distinct characteristics of each cell type. Here, we present a protocol for preparing a single-cell suspension of the zebrafish embryonic whole heart, detailing steps for heart extraction, cell dissociation, quantification, and quality assessment. This suspension is compatible with downstream analysis on various single-cell platforms.For details on the use and execution of this protocol, please refer to Abu Nahia et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007512Cell BiologySingle cellDevelopmental biologyGenomics |
spellingShingle | Karim Abu Nahia Cecilia Lanny Winata Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses STAR Protocols Cell Biology Single cell Developmental biology Genomics |
title | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
title_full | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
title_fullStr | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
title_full_unstemmed | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
title_short | Protocol for the preparation of zebrafish whole heart cell suspension for single-cell analyses |
title_sort | protocol for the preparation of zebrafish whole heart cell suspension for single cell analyses |
topic | Cell Biology Single cell Developmental biology Genomics |
url | http://www.sciencedirect.com/science/article/pii/S2666166724007512 |
work_keys_str_mv | AT karimabunahia protocolforthepreparationofzebrafishwholeheartcellsuspensionforsinglecellanalyses AT cecilialannywinata protocolforthepreparationofzebrafishwholeheartcellsuspensionforsinglecellanalyses |