Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury
The peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist rosiglitazone inhibits NF-κB expression and endogenous neural stem cell differentiation into neurons and reduces the inflammatory cascade after spinal cord injury (SCI). The aim of this study was to explore the mechanisms underlyi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2018-01-01
|
| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2018/3897478 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554300988981248 |
|---|---|
| author | Qing-qi Meng Wei Lei Hao Chen Zhen-cheng Feng Li-qiong Hu Xing-liang Zhang Siming Li |
| author_facet | Qing-qi Meng Wei Lei Hao Chen Zhen-cheng Feng Li-qiong Hu Xing-liang Zhang Siming Li |
| author_sort | Qing-qi Meng |
| collection | DOAJ |
| description | The peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist rosiglitazone inhibits NF-κB expression and endogenous neural stem cell differentiation into neurons and reduces the inflammatory cascade after spinal cord injury (SCI). The aim of this study was to explore the mechanisms underlying rosiglitazone-mediated neuroprotective effects and regulation of the balance between the inflammatory cascade and generation of endogenous spinal cord neurons by using a spinal cord-derived neural stem cell culture system as well as SD rat SCI model. Activation of PPAR-γ could promote neural stem cell proliferation and inhibit PKA expression and neuronal formation in vitro. In the SD rat SCI model, the rosiglitazone + forskolin group showed better locomotor recovery compared to the rosiglitazone and forskolin groups. MAP2 expression was higher in the rosiglitazone + forskolin group than in the rosiglitazone group, NF-κB expression was lower in the rosiglitazone + forskolin group than in the forskolin group, and NeuN expression was higher in the rosiglitazone + forskolin group than in the forskolin group. PPAR-γ activation likely inhibits NF-κB, thereby reducing the inflammatory cascade, and PKA activation likely promotes neuronal cell regeneration. |
| format | Article |
| id | doaj-art-9689a1cba8734170827b684debeb7768 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-9689a1cba8734170827b684debeb77682025-02-03T05:51:56ZengWileyPPAR Research1687-47571687-47652018-01-01201810.1155/2018/38974783897478Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord InjuryQing-qi Meng0Wei Lei1Hao Chen2Zhen-cheng Feng3Li-qiong Hu4Xing-liang Zhang5Siming Li6Department of Orthopedics, Guangzhou Red Cross Hospital, Jinan University, 396 Tongfu Road, Guangzhou 510120, ChinaLaboratory Research Center, Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, Guangzhou 51000, ChinaDepartment of Orthopedics, Guangzhou Red Cross Hospital, Jinan University, 396 Tongfu Road, Guangzhou 510120, ChinaDepartment of Orthopedics, Guangzhou Red Cross Hospital, Jinan University, 396 Tongfu Road, Guangzhou 510120, ChinaLaboratory Research Center, Guangdong Medical University, Zhanjiang 524001, ChinaDepartment of Orthopedics, Guangzhou Red Cross Hospital, Jinan University, 396 Tongfu Road, Guangzhou 510120, ChinaThe peroxisome proliferator-activated receptor gamma (PPAR-γ) agonist rosiglitazone inhibits NF-κB expression and endogenous neural stem cell differentiation into neurons and reduces the inflammatory cascade after spinal cord injury (SCI). The aim of this study was to explore the mechanisms underlying rosiglitazone-mediated neuroprotective effects and regulation of the balance between the inflammatory cascade and generation of endogenous spinal cord neurons by using a spinal cord-derived neural stem cell culture system as well as SD rat SCI model. Activation of PPAR-γ could promote neural stem cell proliferation and inhibit PKA expression and neuronal formation in vitro. In the SD rat SCI model, the rosiglitazone + forskolin group showed better locomotor recovery compared to the rosiglitazone and forskolin groups. MAP2 expression was higher in the rosiglitazone + forskolin group than in the rosiglitazone group, NF-κB expression was lower in the rosiglitazone + forskolin group than in the forskolin group, and NeuN expression was higher in the rosiglitazone + forskolin group than in the forskolin group. PPAR-γ activation likely inhibits NF-κB, thereby reducing the inflammatory cascade, and PKA activation likely promotes neuronal cell regeneration.http://dx.doi.org/10.1155/2018/3897478 |
| spellingShingle | Qing-qi Meng Wei Lei Hao Chen Zhen-cheng Feng Li-qiong Hu Xing-liang Zhang Siming Li Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury PPAR Research |
| title | Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury |
| title_full | Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury |
| title_fullStr | Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury |
| title_full_unstemmed | Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury |
| title_short | Combined Rosiglitazone and Forskolin Have Neuroprotective Effects in SD Rats after Spinal Cord Injury |
| title_sort | combined rosiglitazone and forskolin have neuroprotective effects in sd rats after spinal cord injury |
| url | http://dx.doi.org/10.1155/2018/3897478 |
| work_keys_str_mv | AT qingqimeng combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury AT weilei combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury AT haochen combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury AT zhenchengfeng combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury AT liqionghu combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury AT xingliangzhang combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury AT simingli combinedrosiglitazoneandforskolinhaveneuroprotectiveeffectsinsdratsafterspinalcordinjury |