Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata
The present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) ass...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/787812 |
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| author | Amira A. T. Al-Hosary Jabbar Ahmed Ann Nordengrahn Malik Merza |
| author_facet | Amira A. T. Al-Hosary Jabbar Ahmed Ann Nordengrahn Malik Merza |
| author_sort | Amira A. T. Al-Hosary |
| collection | DOAJ |
| description | The present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. A total of 468 blood samples as well as serum samples were randomly collected from cattle and tested in the PCR as well as in the ELISA developed in this study. Moreover, all samples were also analyzed by conventional Giemsa-stained blood smear. The results of this study revealed a good correlation between the results obtained by PCR and the ELISA, whereas all PCR positive samples scored correctly positive in the ELISA and 73 of the 125 PCR negative samples scored correctly negative. Taken together, a sensitivity of 91.25% and a specificity of 78.4% were recorded, when compared to the PCR data. In conclusion, the SVANOVIR Theileria annulata-Ab is a suitable diagnostic assay for use in the diagnosis and epidemiological surveys of Theileria annulata infection in chronic and carrier animals. |
| format | Article |
| id | doaj-art-967fc0448a914dcebde47c868f7e311e |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-967fc0448a914dcebde47c868f7e311e2025-02-03T06:08:36ZengWileyJournal of Parasitology Research2090-00232090-00312015-01-01201510.1155/2015/787812787812Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulataAmira A. T. Al-Hosary0Jabbar Ahmed1Ann Nordengrahn2Malik Merza3Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Assiut University, Assiut 71526, EgyptInstitute of Parasitology and Tropical Veterinary Medicine, Faculty of Veterinary Medicine, Free University of Berlin, 14195 Berlin, GermanyBoehringer Ingelheim Svanova, Uppsala Business Park, P.O. Box 1545, 75145 Uppsala, SwedenBoehringer Ingelheim Svanova, Uppsala Business Park, P.O. Box 1545, 75145 Uppsala, SwedenThe present study assesses the efficacy of SVANOVIR Theileria annulata-Ab, the first commercial ELISA kit for the diagnosis of Theileria annulata infection in cattle based on a recombinant protein known as T. annulata surface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending on T. annulata merozoite surface antigen (Tams-1) was applied. A total of 468 blood samples as well as serum samples were randomly collected from cattle and tested in the PCR as well as in the ELISA developed in this study. Moreover, all samples were also analyzed by conventional Giemsa-stained blood smear. The results of this study revealed a good correlation between the results obtained by PCR and the ELISA, whereas all PCR positive samples scored correctly positive in the ELISA and 73 of the 125 PCR negative samples scored correctly negative. Taken together, a sensitivity of 91.25% and a specificity of 78.4% were recorded, when compared to the PCR data. In conclusion, the SVANOVIR Theileria annulata-Ab is a suitable diagnostic assay for use in the diagnosis and epidemiological surveys of Theileria annulata infection in chronic and carrier animals.http://dx.doi.org/10.1155/2015/787812 |
| spellingShingle | Amira A. T. Al-Hosary Jabbar Ahmed Ann Nordengrahn Malik Merza Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata Journal of Parasitology Research |
| title | Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata |
| title_full | Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata |
| title_fullStr | Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata |
| title_full_unstemmed | Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata |
| title_short | Assessment of the First Commercial ELISA Kit for the Diagnosis of Theileria annulata |
| title_sort | assessment of the first commercial elisa kit for the diagnosis of theileria annulata |
| url | http://dx.doi.org/10.1155/2015/787812 |
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