Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma
Background. The Nuclear protein 1 gene was first discovered in acute pancreatitis and functions as an oncogene in cancer progression and drug resistance. However, the role of Nuclear protein 1 in bladder transitional cell carcinoma (BTCC) is still unclear. Methods. The Cancer Genome Atlas database a...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2023/6797694 |
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| _version_ | 1832559637861236736 |
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| author | Chao Lu Shenglin Gao Li Zhang Xiaokai Shi Yin Chen Shuzhang Wei Li Zuo Lifeng Zhang |
| author_facet | Chao Lu Shenglin Gao Li Zhang Xiaokai Shi Yin Chen Shuzhang Wei Li Zuo Lifeng Zhang |
| author_sort | Chao Lu |
| collection | DOAJ |
| description | Background. The Nuclear protein 1 gene was first discovered in acute pancreatitis and functions as an oncogene in cancer progression and drug resistance. However, the role of Nuclear protein 1 in bladder transitional cell carcinoma (BTCC) is still unclear. Methods. The Cancer Genome Atlas database and immunohistochemical analysis were adopted to evaluate Nuclear protein 1 expression in BTCC. We applied lentivirus-mediated small-interfering RNA to down-regulate the expression of Nuclear protein 1 in BTCC cell lines. We further performed an Affymetrix microarray and Gene Set Enrichment Analysis (GSEA) to assess the genes and signaling pathways related to Nuclear protein 1. Results. We found that Nuclear protein 1 expression was up-regulated in BTCC and positively related to the degree of BTCC malignancy. Compared with Caucasian patients with BTCC, Nuclear protein 1 expression was attenuated in Asian patients. The Affymetrix microarray showed that lipopolysaccharide was the upstream regulatory factor of Nuclear protein 1 in BTCC. The GSEA indicated that Nuclear protein 1 expression was associated with signaling pathways in cancer, peroxisome proliferator-activated receptor (PPAR) pathways, and RNA degradation. The expression of Nuclear protein 1 was negatively correlated with PPARG (R=−0.290, P<0.001), but not with PPARA (R=0.047, P=0.344) and PPARD (R=−0.055, P=0.260). Conclusions. The study findings indicate that Nuclear protein 1 is positively associated with the malignancy degree of BTCC and that Nuclear protein 1 expression is negatively correlated with PPARG. |
| format | Article |
| id | doaj-art-96730e4831ec4cddb4606c5c2d0fe2c1 |
| institution | Kabale University |
| issn | 1687-4765 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-96730e4831ec4cddb4606c5c2d0fe2c12025-02-03T01:29:33ZengWileyPPAR Research1687-47652023-01-01202310.1155/2023/6797694Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell CarcinomaChao Lu0Shenglin Gao1Li Zhang2Xiaokai Shi3Yin Chen4Shuzhang Wei5Li Zuo6Lifeng Zhang7Department of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyDepartment of UrologyBackground. The Nuclear protein 1 gene was first discovered in acute pancreatitis and functions as an oncogene in cancer progression and drug resistance. However, the role of Nuclear protein 1 in bladder transitional cell carcinoma (BTCC) is still unclear. Methods. The Cancer Genome Atlas database and immunohistochemical analysis were adopted to evaluate Nuclear protein 1 expression in BTCC. We applied lentivirus-mediated small-interfering RNA to down-regulate the expression of Nuclear protein 1 in BTCC cell lines. We further performed an Affymetrix microarray and Gene Set Enrichment Analysis (GSEA) to assess the genes and signaling pathways related to Nuclear protein 1. Results. We found that Nuclear protein 1 expression was up-regulated in BTCC and positively related to the degree of BTCC malignancy. Compared with Caucasian patients with BTCC, Nuclear protein 1 expression was attenuated in Asian patients. The Affymetrix microarray showed that lipopolysaccharide was the upstream regulatory factor of Nuclear protein 1 in BTCC. The GSEA indicated that Nuclear protein 1 expression was associated with signaling pathways in cancer, peroxisome proliferator-activated receptor (PPAR) pathways, and RNA degradation. The expression of Nuclear protein 1 was negatively correlated with PPARG (R=−0.290, P<0.001), but not with PPARA (R=0.047, P=0.344) and PPARD (R=−0.055, P=0.260). Conclusions. The study findings indicate that Nuclear protein 1 is positively associated with the malignancy degree of BTCC and that Nuclear protein 1 expression is negatively correlated with PPARG.http://dx.doi.org/10.1155/2023/6797694 |
| spellingShingle | Chao Lu Shenglin Gao Li Zhang Xiaokai Shi Yin Chen Shuzhang Wei Li Zuo Lifeng Zhang Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma PPAR Research |
| title | Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma |
| title_full | Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma |
| title_fullStr | Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma |
| title_full_unstemmed | Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma |
| title_short | Nuclear Protein 1 Expression Is Associated with PPARG in Bladder Transitional Cell Carcinoma |
| title_sort | nuclear protein 1 expression is associated with pparg in bladder transitional cell carcinoma |
| url | http://dx.doi.org/10.1155/2023/6797694 |
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