Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study
<b>Background/Objectives</b>: The global spread of carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insuffici...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/14/1/94 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589344697745408 |
|---|---|
| author | Anneloes van Veen Selvi N. Shahab Amber Rijfkogel Anne F. Voor in ’t holt Corné H. W. Klaassen Margreet C. Vos Yulia Rosa Saharman Anis Karuniawati Silvia Zelli Desy De Lorenzis Giulia Menchinelli Giulia De Angelis Maurizio Sanguinetti Merel Kemper Anniek E. E. de Jong Sima Mohammadi Valentine Renaud Irena Kukavica-Ibrulj Marianne Potvin Guillaume Q. Nguyen Jeff Gauthier Roger C. Levesque Heike Schmitt Juliëtte A. Severin |
| author_facet | Anneloes van Veen Selvi N. Shahab Amber Rijfkogel Anne F. Voor in ’t holt Corné H. W. Klaassen Margreet C. Vos Yulia Rosa Saharman Anis Karuniawati Silvia Zelli Desy De Lorenzis Giulia Menchinelli Giulia De Angelis Maurizio Sanguinetti Merel Kemper Anniek E. E. de Jong Sima Mohammadi Valentine Renaud Irena Kukavica-Ibrulj Marianne Potvin Guillaume Q. Nguyen Jeff Gauthier Roger C. Levesque Heike Schmitt Juliëtte A. Severin |
| author_sort | Anneloes van Veen |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: The global spread of carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant <i>Pseudomonas aeruginosa</i>”) is to develop a globally applicable surveillance strategy. <b>Methods</b>: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals’ (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. <b>Conclusions</b>: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA. |
| format | Article |
| id | doaj-art-961b8291a46b4ebb8fa57ecaf206246c |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-961b8291a46b4ebb8fa57ecaf206246c2025-01-24T13:18:58ZengMDPI AGAntibiotics2079-63822025-01-011419410.3390/antibiotics14010094Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN StudyAnneloes van Veen0Selvi N. Shahab1Amber Rijfkogel2Anne F. Voor in ’t holt3Corné H. W. Klaassen4Margreet C. Vos5Yulia Rosa Saharman6Anis Karuniawati7Silvia Zelli8Desy De Lorenzis9Giulia Menchinelli10Giulia De Angelis11Maurizio Sanguinetti12Merel Kemper13Anniek E. E. de Jong14Sima Mohammadi15Valentine Renaud16Irena Kukavica-Ibrulj17Marianne Potvin18Guillaume Q. Nguyen19Jeff Gauthier20Roger C. Levesque21Heike Schmitt22Juliëtte A. Severin23Department of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The NetherlandsDepartment of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta 10430, IndonesiaDepartment of Clinical Microbiology, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta 10430, IndonesiaDipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Roma, ItalyDipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Roma, ItalyDipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Roma, ItalyDipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Roma, ItalyDipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario Agostino Gemelli IRCCS, 00168 Roma, ItalyCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), 3720 BA Bilthoven, The NetherlandsDeltares, 2629 HV Delft, The NetherlandsInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaInstitut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Quebec City, QC G1V 0A6, CanadaCentre for Infectious Disease Control, National Institute for Public Health and the Environment (RIVM), 3720 BA Bilthoven, The NetherlandsDepartment of Medical Microbiology and Infectious Diseases, Erasmus MC University Medical Center, 3015 GD Rotterdam, The Netherlands<b>Background/Objectives</b>: The global spread of carbapenem-resistant <i>Pseudomonas aeruginosa</i> (CRPA) warrants collaborative action. Guidance should come from integrated One Health surveillance; however, a surveillance strategy is currently unavailable due to insufficient knowledge on the sources and transmission routes of CRPA. The aim of the SAMPAN study (“A Smart Surveillance Strategy for Carbapenem-resistant <i>Pseudomonas aeruginosa</i>”) is to develop a globally applicable surveillance strategy. <b>Methods</b>: First, an international cross-sectional study will be conducted to investigate CRPA in clinical and environmental settings in Rotterdam (The Netherlands), Rome (Italy), and Jakarta (Indonesia). Screening cultures and risk factor questionnaires will be taken from healthy individuals and patients upon hospital admission. Clinical CRPA isolates will also be included. Additionally, samples will be taken twice from wet hospital environments and monthly from the hospitals’ (drinking) water system, hospital and municipal wastewater treatment plants, and receiving rivers. Whole-genome sequencing will be performed to characterize CRPA isolates and determine the genetic relatedness among the isolates from different reservoirs. Findings from the cross-sectional study, combined with expert elicitation using a Delphi method, will serve as the input for the surveillance strategy. <b>Conclusions</b>: The SAMPAN study will provide a broader understanding of the sources and transmission routes of CRPA. Therewith, the development of a globally applicable smart surveillance strategy will be made possible, delivering information that is needed to guide actions against the spread of CRPA.https://www.mdpi.com/2079-6382/14/1/94antimicrobial drug resistancecarbapenemshospitalshumansOne Health<i>Pseudomonas aeruginosa</i> |
| spellingShingle | Anneloes van Veen Selvi N. Shahab Amber Rijfkogel Anne F. Voor in ’t holt Corné H. W. Klaassen Margreet C. Vos Yulia Rosa Saharman Anis Karuniawati Silvia Zelli Desy De Lorenzis Giulia Menchinelli Giulia De Angelis Maurizio Sanguinetti Merel Kemper Anniek E. E. de Jong Sima Mohammadi Valentine Renaud Irena Kukavica-Ibrulj Marianne Potvin Guillaume Q. Nguyen Jeff Gauthier Roger C. Levesque Heike Schmitt Juliëtte A. Severin Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study Antibiotics antimicrobial drug resistance carbapenems hospitals humans One Health <i>Pseudomonas aeruginosa</i> |
| title | Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study |
| title_full | Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study |
| title_fullStr | Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study |
| title_full_unstemmed | Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study |
| title_short | Sources and Transmission Routes of Carbapenem-Resistant <i>Pseudomonas aeruginosa</i>: Study Design and Methodology of the SAMPAN Study |
| title_sort | sources and transmission routes of carbapenem resistant i pseudomonas aeruginosa i study design and methodology of the sampan study |
| topic | antimicrobial drug resistance carbapenems hospitals humans One Health <i>Pseudomonas aeruginosa</i> |
| url | https://www.mdpi.com/2079-6382/14/1/94 |
| work_keys_str_mv | AT anneloesvanveen sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT selvinshahab sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT amberrijfkogel sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT annefvoorintholt sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT cornehwklaassen sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT margreetcvos sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT yuliarosasaharman sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT aniskaruniawati sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT silviazelli sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT desydelorenzis sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT giuliamenchinelli sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT giuliadeangelis sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT mauriziosanguinetti sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT merelkemper sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT anniekeedejong sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT simamohammadi sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT valentinerenaud sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT irenakukavicaibrulj sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT mariannepotvin sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT guillaumeqnguyen sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT jeffgauthier sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT rogerclevesque sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT heikeschmitt sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy AT julietteaseverin sourcesandtransmissionroutesofcarbapenemresistantipseudomonasaeruginosaistudydesignandmethodologyofthesampanstudy |