Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin
Background/Aim. Uncomplicated diverticular disease (UDD) is a frequent condition in adults. The pathogenesis of symptoms remains unknown. Bacteria are able to interact with Toll-like receptors (TLRs) and to induce inflammation through both innate immunity and T-cell recruitment. We investigated the...
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2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/696812 |
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| author | Rossella Cianci Simona Frosali Danilo Pagliari Paola Cesaro Lucio Petruzziello Fabio Casciano Raffaele Landolfi Guido Costamagna Franco Pandolfi |
| author_facet | Rossella Cianci Simona Frosali Danilo Pagliari Paola Cesaro Lucio Petruzziello Fabio Casciano Raffaele Landolfi Guido Costamagna Franco Pandolfi |
| author_sort | Rossella Cianci |
| collection | DOAJ |
| description | Background/Aim. Uncomplicated diverticular disease (UDD) is a frequent condition in adults. The pathogenesis of symptoms remains unknown. Bacteria are able to interact with Toll-like receptors (TLRs) and to induce inflammation through both innate immunity and T-cell recruitment. We investigated the pattern of TLRs 2 and 4 and the intestinal homing in patients with UDD before and after a course of Rifaximin. Methods. Forty consecutive patients with UDD and 20 healthy asymptomatic subjects were enrolled. Among UDD patients, 20 were assigned to a 2-month course of treatment with Rifaximin 1.2 g/day for 15 days/month and 20 received placebo. Blood sample and colonic biopsies were obtained from patients and controls. The samples were collected and analyzed at baseline and at the end of treatment. Flow cytometry was performed using monoclonal antibodies (CD3, CD4, CD8, CD103, TCR-gamma/delta, CD14, TLR2, and TLR4). Results. In UDD, TLR2 and TLR4 expression on immune cell subpopulations from blood and mucosa of the affected colon are altered as compared with controls. Rifaximin treatment induced significant modifications of altered conditions. Conclusions. Our data show the role of TLRs in the development of inflammation in UDD. TLRs distribution is altered in UDD and these alterations are reversed after antibiotic treatment. This trial is registered with ClinicalTrials.gov:
NCT02068482. |
| format | Article |
| id | doaj-art-95d8d7ea308b4b7f8eecd2cfa7f7a0ff |
| institution | DOAJ |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-95d8d7ea308b4b7f8eecd2cfa7f7a0ff2025-08-20T03:20:33ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/696812696812Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after RifaximinRossella Cianci0Simona Frosali1Danilo Pagliari2Paola Cesaro3Lucio Petruzziello4Fabio Casciano5Raffaele Landolfi6Guido Costamagna7Franco Pandolfi8Department of Medical Science, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyDepartment of Medical Science, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyDepartment of Medical Science, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyEndoscopy Unit, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyEndoscopy Unit, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyDepartment of Medical Science, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyDepartment of Medical Science, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyEndoscopy Unit, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyDepartment of Medical Science, Catholic University of the Sacred Heart, ‘A. Gemelli’ University Hospital, Largo A. Gemelli, 8-00168 Rome, ItalyBackground/Aim. Uncomplicated diverticular disease (UDD) is a frequent condition in adults. The pathogenesis of symptoms remains unknown. Bacteria are able to interact with Toll-like receptors (TLRs) and to induce inflammation through both innate immunity and T-cell recruitment. We investigated the pattern of TLRs 2 and 4 and the intestinal homing in patients with UDD before and after a course of Rifaximin. Methods. Forty consecutive patients with UDD and 20 healthy asymptomatic subjects were enrolled. Among UDD patients, 20 were assigned to a 2-month course of treatment with Rifaximin 1.2 g/day for 15 days/month and 20 received placebo. Blood sample and colonic biopsies were obtained from patients and controls. The samples were collected and analyzed at baseline and at the end of treatment. Flow cytometry was performed using monoclonal antibodies (CD3, CD4, CD8, CD103, TCR-gamma/delta, CD14, TLR2, and TLR4). Results. In UDD, TLR2 and TLR4 expression on immune cell subpopulations from blood and mucosa of the affected colon are altered as compared with controls. Rifaximin treatment induced significant modifications of altered conditions. Conclusions. Our data show the role of TLRs in the development of inflammation in UDD. TLRs distribution is altered in UDD and these alterations are reversed after antibiotic treatment. This trial is registered with ClinicalTrials.gov: NCT02068482.http://dx.doi.org/10.1155/2014/696812 |
| spellingShingle | Rossella Cianci Simona Frosali Danilo Pagliari Paola Cesaro Lucio Petruzziello Fabio Casciano Raffaele Landolfi Guido Costamagna Franco Pandolfi Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin Journal of Immunology Research |
| title | Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin |
| title_full | Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin |
| title_fullStr | Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin |
| title_full_unstemmed | Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin |
| title_short | Uncomplicated Diverticular Disease: Innate and Adaptive Immunity in Human Gut Mucosa before and after Rifaximin |
| title_sort | uncomplicated diverticular disease innate and adaptive immunity in human gut mucosa before and after rifaximin |
| url | http://dx.doi.org/10.1155/2014/696812 |
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