PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation
Peptidyl arginine deiminase IV (PAD 4) is the responsible enzyme for a posttranslational modification called citrullination, originating the antigenic determinant recognized by anti-cyclic citrullinated peptide antibodies (ACPA). Four SNPs (single nucleotide polymorphisms) have been described in PAD...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2013/383681 |
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| author | Maria Guadalupe Zavala-Cerna Norma Guadalupe Gonzalez-Montoya Arnulfo Nava Jorge I. Gamez-Nava Maria Cristina Moran-Moguel Roberto Carlos Rosales-Gomez Susan Andrea Gutierrez-Rubio Jose Sanchez-Corona Laura Gonzalez-Lopez Ingrid Patricia Davalos-Rodriguez Mario Salazar-Paramo |
| author_facet | Maria Guadalupe Zavala-Cerna Norma Guadalupe Gonzalez-Montoya Arnulfo Nava Jorge I. Gamez-Nava Maria Cristina Moran-Moguel Roberto Carlos Rosales-Gomez Susan Andrea Gutierrez-Rubio Jose Sanchez-Corona Laura Gonzalez-Lopez Ingrid Patricia Davalos-Rodriguez Mario Salazar-Paramo |
| author_sort | Maria Guadalupe Zavala-Cerna |
| collection | DOAJ |
| description | Peptidyl arginine deiminase IV (PAD 4) is the responsible enzyme for a posttranslational modification called citrullination, originating the antigenic determinant recognized by anti-cyclic citrullinated peptide antibodies (ACPA). Four SNPs (single nucleotide polymorphisms) have been described in PADI4 gene to form a susceptibility haplotype for rheumatoid arthritis (RA); nevertheless, results in association studies appear contradictory in different populations. The aim of the study was to analyze if the presence of three SNPs in PADI4 gene susceptibility haplotype (GTG) is associated with ACPA positivity in patients with RA. This was a cross-sectional study that included 86 RA patients and 98 healthy controls. Polymorphisms PADI4_89, PADI4_90, and PADI4_92 in the PADI4 gene were genotyped. The susceptibility haplotype (GTG) was more frequent in RA patients; interestingly, we found a new haplotype associated with RA with a higher frequency (GTC). There were no associations between polymorphisms and high scores in Spanish HAQ-DI and DAS-28, but we did find an association between RARBIS index and PADI4_89, PADI4_90 polymorphisms. We could not confirm an association between susceptibility haplotype presence and ACPA positivity. Further evidence about proteomic expression of this gene will determine its participation in antigenic generation and autoimmunity. |
| format | Article |
| id | doaj-art-95d17dace1024092a69b44e5621f9330 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-95d17dace1024092a69b44e5621f93302025-02-03T06:10:49ZengWileyClinical and Developmental Immunology1740-25221740-25302013-01-01201310.1155/2013/383681383681PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen ModulationMaria Guadalupe Zavala-Cerna0Norma Guadalupe Gonzalez-Montoya1Arnulfo Nava2Jorge I. Gamez-Nava3Maria Cristina Moran-Moguel4Roberto Carlos Rosales-Gomez5Susan Andrea Gutierrez-Rubio6Jose Sanchez-Corona7Laura Gonzalez-Lopez8Ingrid Patricia Davalos-Rodriguez9Mario Salazar-Paramo10Programa Internacional del ICB, Facultad de Medicina, Universidad Autónoma de Guadalajara, Guadalajara, JAL, MexicoUnidad de Investigación en Epidemiología Clínica, Hospital de Especialidades, Centro Médico Nacional de Occidente del Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoPrograma Internacional del ICB, Facultad de Medicina, Universidad Autónoma de Guadalajara, Guadalajara, JAL, MexicoUnidad de Investigación en Epidemiología Clínica, Hospital de Especialidades, Centro Médico Nacional de Occidente del Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoDivisión de Medicina Molecular del Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoDivisión de Medicina Molecular del Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoDivisión de Medicina Molecular del Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoDivisión de Medicina Molecular del Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoDepartamento de Medicina Interna-Reumatología del Hospital General Regional No. 110, Instituto Mexicano del Seguro Social, Guadalajara, JAL, MexicoDivisión de Genética, CIBO, IMSS and Instituto de Genética Humana, CUCS Universidad de Guadalajara, Guadalajara, JAL, MexicoDivisión de Investigación en Salud, UMAE, Hospital de Especialidades, CMNO, IMSS and Departamento de Fisiología, CUCS, Universidad de Guadalajara, MexicoPeptidyl arginine deiminase IV (PAD 4) is the responsible enzyme for a posttranslational modification called citrullination, originating the antigenic determinant recognized by anti-cyclic citrullinated peptide antibodies (ACPA). Four SNPs (single nucleotide polymorphisms) have been described in PADI4 gene to form a susceptibility haplotype for rheumatoid arthritis (RA); nevertheless, results in association studies appear contradictory in different populations. The aim of the study was to analyze if the presence of three SNPs in PADI4 gene susceptibility haplotype (GTG) is associated with ACPA positivity in patients with RA. This was a cross-sectional study that included 86 RA patients and 98 healthy controls. Polymorphisms PADI4_89, PADI4_90, and PADI4_92 in the PADI4 gene were genotyped. The susceptibility haplotype (GTG) was more frequent in RA patients; interestingly, we found a new haplotype associated with RA with a higher frequency (GTC). There were no associations between polymorphisms and high scores in Spanish HAQ-DI and DAS-28, but we did find an association between RARBIS index and PADI4_89, PADI4_90 polymorphisms. We could not confirm an association between susceptibility haplotype presence and ACPA positivity. Further evidence about proteomic expression of this gene will determine its participation in antigenic generation and autoimmunity.http://dx.doi.org/10.1155/2013/383681 |
| spellingShingle | Maria Guadalupe Zavala-Cerna Norma Guadalupe Gonzalez-Montoya Arnulfo Nava Jorge I. Gamez-Nava Maria Cristina Moran-Moguel Roberto Carlos Rosales-Gomez Susan Andrea Gutierrez-Rubio Jose Sanchez-Corona Laura Gonzalez-Lopez Ingrid Patricia Davalos-Rodriguez Mario Salazar-Paramo PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation Clinical and Developmental Immunology |
| title | PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation |
| title_full | PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation |
| title_fullStr | PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation |
| title_full_unstemmed | PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation |
| title_short | PADI4 Haplotypes in Association with RA Mexican Patients, a New Prospect for Antigen Modulation |
| title_sort | padi4 haplotypes in association with ra mexican patients a new prospect for antigen modulation |
| url | http://dx.doi.org/10.1155/2013/383681 |
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