Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring
Lonicerae japonicae Flos (LF) is a kind of healthcare food with hepatoprotective function. This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF. Fingerprints of ten batches of LF were established by UPLC-PDA. The inhibi...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001252 |
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| author | Song Wang Lin Yang Ajiao Hou Songtao Liu Liu Yang Haixue Kuang Hai Jiang |
| author_facet | Song Wang Lin Yang Ajiao Hou Songtao Liu Liu Yang Haixue Kuang Hai Jiang |
| author_sort | Song Wang |
| collection | DOAJ |
| description | Lonicerae japonicae Flos (LF) is a kind of healthcare food with hepatoprotective function. This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF. Fingerprints of ten batches of LF were established by UPLC-PDA. The inhibitory levels of AST and ALT were used as pharmacological indexes, and secoxyloganin, isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis (GRA) and partial least squares regression analysis (PLSR). Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds. Molecular docking is used to explore the interaction between small molecules and proteins. This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF, which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food. |
| format | Article |
| id | doaj-art-95cd149e46524ce2b6133e2b03d1deec |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-95cd149e46524ce2b6133e2b03d1deec2025-02-03T05:14:34ZengTsinghua University PressFood Science and Human Wellness2213-45302023-01-01121283294Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploringSong Wang0Lin Yang1Ajiao Hou2Songtao Liu3Liu Yang4Haixue Kuang5Hai Jiang6Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China; School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China; Corresponding authors at: Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, ChinaKey Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China; Corresponding authors at: Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, ChinaLonicerae japonicae Flos (LF) is a kind of healthcare food with hepatoprotective function. This study was designed to explore the spectrum-effect relationships between UPLC fingerprints and the hepatoprotective effects of LF. Fingerprints of ten batches of LF were established by UPLC-PDA. The inhibitory levels of AST and ALT were used as pharmacological indexes, and secoxyloganin, isochlorogenic acid A and isochlorogenic acid C were screened as hepatoprotective active compounds by grey relational analysis (GRA) and partial least squares regression analysis (PLSR). Caspase-3 was obtained by network pharmacology as a key target of hepatoprotective active compounds. Molecular docking is used to explore the interaction between small molecules and proteins. This work provided a general model of the combination of UPLC-PDA and hepatoprotective effect to study the spectrum-effect relationship of LF, which can be used to considerable methods and insight for the fundamental research of the material basis of similar healthcare food.http://www.sciencedirect.com/science/article/pii/S2213453022001252Lonicerae japonicae FlosHepatoprotective effectSpectrum-effect relationshipMolecular dockingMechanism |
| spellingShingle | Song Wang Lin Yang Ajiao Hou Songtao Liu Liu Yang Haixue Kuang Hai Jiang Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring Food Science and Human Wellness Lonicerae japonicae Flos Hepatoprotective effect Spectrum-effect relationship Molecular docking Mechanism |
| title | Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring |
| title_full | Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring |
| title_fullStr | Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring |
| title_full_unstemmed | Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring |
| title_short | Screening hepatoprotective effective components of Lonicerae japonica Flos based on the spectrum-effect relationship and its mechanism exploring |
| title_sort | screening hepatoprotective effective components of lonicerae japonica flos based on the spectrum effect relationship and its mechanism exploring |
| topic | Lonicerae japonicae Flos Hepatoprotective effect Spectrum-effect relationship Molecular docking Mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001252 |
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