Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo
Objective. To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients. Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia including E. bieneusi and E. intestinalis was perform...
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| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2012/278028 |
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| author | Roger Wumba Menotti Jean Longo-Mbenza Benjamin Mandina Madone Kintoki Fabien Zanga Josué Sala Jean Kendjo Eric Guillo-Olczyk AC Thellier Marc |
| author_facet | Roger Wumba Menotti Jean Longo-Mbenza Benjamin Mandina Madone Kintoki Fabien Zanga Josué Sala Jean Kendjo Eric Guillo-Olczyk AC Thellier Marc |
| author_sort | Roger Wumba |
| collection | DOAJ |
| description | Objective. To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients.
Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia including E. bieneusi and E. intestinalis was performed
in 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS region of E. bieneusi. PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages. The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard. Results. Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of E. bieneusi was 7.9% (n=19) among the 19 E. bieneusi, one was coinfected with E. intestinalis. In 19 E. bieneusi persons using PCR-RFLP method, 5 type I strains of E. bieneusi (26.3%) and 5 type IV strains of E. bieneusi (26.3%) were identified. The sensitivity of IFI-AcM was poor as estimated 42.1%.
Conclusion. Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains. |
| format | Article |
| id | doaj-art-95c1c50987634c5c94ab6a154c09a519 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-95c1c50987634c5c94ab6a154c09a5192025-02-03T00:59:30ZengWileyJournal of Parasitology Research2090-00232090-00312012-01-01201210.1155/2012/278028278028Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of CongoRoger Wumba0Menotti Jean1Longo-Mbenza Benjamin2Mandina Madone3Kintoki Fabien4Zanga Josué5Sala Jean6Kendjo Eric7Guillo-Olczyk AC8Thellier Marc9Département de Médecine Tropicale, Maladies Infectieuses et Parasitaires, Cliniques Universitaires de Kinshasa, Université de Kinshasa, 747 Kinshasa XI, Democratic Republic of CongoService de Parasitologie-Mycologie, Hôpital Saint-Louis, Assistance Publique-Hôpitaux de Paris et Faculté de Médecine Lariboisière-Saint-Louis, Université Paris VII, 75010 Paris, FranceFaculty of Health Sciences, Walter Sisulu University, Private Bag XI, Mthatha, Eastern Cape 5117, South AfricaDepartment of Internal Medicine, University of Kinshasa, 783 Kinshasa XI, Democratic Republic of CongoDepartment of Internal Medicine, University of Kinshasa, 783 Kinshasa XI, Democratic Republic of CongoDépartement de Médecine Tropicale, Maladies Infectieuses et Parasitaires, Cliniques Universitaires de Kinshasa, Université de Kinshasa, 747 Kinshasa XI, Democratic Republic of CongoDépartement de Médecine Tropicale, Maladies Infectieuses et Parasitaires, Cliniques Universitaires de Kinshasa, Université de Kinshasa, 747 Kinshasa XI, Democratic Republic of CongoNational Center for Malaria Research, AP-HP, CHU Pitié Salpetrière, 75013 Paris, FranceAP-HP, Groupe hospitalier Pitié-Salpêtrière, Service de Parasitologie-Mycologie, Université Pierre et Marie Curie, 75013 Paris, FranceNational Center for Malaria Research, AP-HP, CHU Pitié Salpetrière, 75013 Paris, FranceObjective. To determine the prevalence and the genotypes of Enterocytozoon bieneusi in stool specimens from HIV patients. Methods. This cross-sectional study was carried out in Kinshasa hospitals between 2009 and 2012. Detection of microsporidia including E. bieneusi and E. intestinalis was performed in 242 HIV-infected patients. Typing was based on DNA polymorphism of the ribosomal DNA ITS region of E. bieneusi. PCRRFLP generated with two restriction enzymes (Nla III and Fnu 4HI) in PCR-amplified ITS products for classifying strains into different lineages. The diagnosis performance of the indirect immune-fluorescence-monoclonal antibody (IFI-AcM) was defined in comparison with real-time PCR as the gold standard. Results. Out of 242 HIV-infected patients, using the real-time PCR, the prevalence of E. bieneusi was 7.9% (n=19) among the 19 E. bieneusi, one was coinfected with E. intestinalis. In 19 E. bieneusi persons using PCR-RFLP method, 5 type I strains of E. bieneusi (26.3%) and 5 type IV strains of E. bieneusi (26.3%) were identified. The sensitivity of IFI-AcM was poor as estimated 42.1%. Conclusion. Despite different PCR methods, there is possible association between HIVinfection, geographic location (France, Cameroun, Democratic Republic of Congo), and the concurrence of type I and type IV strains.http://dx.doi.org/10.1155/2012/278028 |
| spellingShingle | Roger Wumba Menotti Jean Longo-Mbenza Benjamin Mandina Madone Kintoki Fabien Zanga Josué Sala Jean Kendjo Eric Guillo-Olczyk AC Thellier Marc Enterocytozoon bieneusi Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo Journal of Parasitology Research |
| title | Enterocytozoon bieneusi
Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo |
| title_full | Enterocytozoon bieneusi
Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo |
| title_fullStr | Enterocytozoon bieneusi
Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo |
| title_full_unstemmed | Enterocytozoon bieneusi
Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo |
| title_short | Enterocytozoon bieneusi
Identification Using Real-Time Polymerase Chain Reaction and Restriction Fragment Length Polymorphism in HIV-Infected Humans from Kinshasa Province of the Democratic Republic of Congo |
| title_sort | enterocytozoon bieneusi identification using real time polymerase chain reaction and restriction fragment length polymorphism in hiv infected humans from kinshasa province of the democratic republic of congo |
| url | http://dx.doi.org/10.1155/2012/278028 |
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