Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment
Heat stress (HS) has become a major concern for the poultry industry in many countries. HS impacts gut health by causing damaged mucosal microstructures, increased oxidative stress, weakened immunity, and heightened permeability to toxins and poultry pathogens. We investigated the potential benefits...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124010964 |
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| author | Sunisa Khongthong Pipat Piewngam Natthrit Roekngam Prapot Maliwan Sonsawan Kongpuckdee Juthatip Jeenkeawpleam Prawit Rodjan |
| author_facet | Sunisa Khongthong Pipat Piewngam Natthrit Roekngam Prapot Maliwan Sonsawan Kongpuckdee Juthatip Jeenkeawpleam Prawit Rodjan |
| author_sort | Sunisa Khongthong |
| collection | DOAJ |
| description | Heat stress (HS) has become a major concern for the poultry industry in many countries. HS impacts gut health by causing damaged mucosal microstructures, increased oxidative stress, weakened immunity, and heightened permeability to toxins and poultry pathogens. We investigated the potential benefits to broiler chickens subjected to HS of dietary supplementation with Bacillus subtilis 14823. Growth performance, gut barrier integrity, and expressions of inflammatory cytokines were analyzed. The results indicated that dietary supplementation with B. subtilis spores at concentrations of 1 × 106 CFU/g of feed (BS6 group) and 1 × 107 CFU/g of feed (BS7 group) improved body weight and body weight gain during d 0–42 (P < 0.05), while the feed intake of the BS7 group was highest (P < 0.05). Additionally, the BS6 group showed a better feed conversion ratio than the control (CON) group (P < 0.05). The BS7 group showed the lowest serum fluorescein isothiocyanate-dextran levels (P < 0.05), and both the BS6 and BS7 groups showed lower corticosterone levels than the CON group (P < 0.05). Additionally, both the BS6 and BS7 groups demonstrated increased villi height and villus height/crypt depth ratio, along with decreased crypt depth in the duodenum and ileum (P < 0.05). However, only the BS7 group exhibited greater improvements than the CON group in the jejunum at d 35. Furthermore, at d 14 and 35, mRNA expressions of occludin, claudin-1, and tight junction protein-1 in the jejunum were upregulated (P < 0.05), and expression levels of five inflammatory cytokine genes were downregulated in the ileum (P < 0.05). Our findings provide new insights and evidence supporting the application of B. subtilis 14823 for enhancing growth performance, gut barrier integrity, and modulating inflammatory cytokines in broilers. |
| format | Article |
| id | doaj-art-95a64116fe3b430799cb8fd42cfb0433 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-95a64116fe3b430799cb8fd42cfb04332025-01-22T05:40:18ZengElsevierPoultry Science0032-57912025-01-011041104518Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environmentSunisa Khongthong0Pipat Piewngam1Natthrit Roekngam2Prapot Maliwan3Sonsawan Kongpuckdee4Juthatip Jeenkeawpleam5Prawit Rodjan6Faculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Thung-Yai, Nakhon Si Thammarat 80240, ThailandPathogen Molecular Genetics Section, Laboratory of Bacteriology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USAFaculty of Veterinary Science, Rajamangala University of Technology Srivijaya, Thung-Yai, Nakhon Si Thammarat 80240, ThailandDepartment of Animal Science, Faculty of Agriculture, Rajamangala University of Technology Srivijaya, Thung-Yai, Nakhon Si Thammarat 80240, ThailandDepartment of Thai Traditional Medicine, Faculty of Health and Sports Science, Thaksin University, Pa Phayom, Phatthalung 93210, ThailandOne Health Research Center, Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, ThailandSchool of Agricultural Technology and Food Industry, Walailak University, Nakhon Si Thammarat 80160, Thailand; Food Technology and Innovation Center of Excellence, Walailak University, Nakhon Si Thammarat 80160, Thailand; Center of Excellence in Innovation on Essential oil, Walailak University, Nakhon Si Thammarat 80160, Thailand; Corresponding author.Heat stress (HS) has become a major concern for the poultry industry in many countries. HS impacts gut health by causing damaged mucosal microstructures, increased oxidative stress, weakened immunity, and heightened permeability to toxins and poultry pathogens. We investigated the potential benefits to broiler chickens subjected to HS of dietary supplementation with Bacillus subtilis 14823. Growth performance, gut barrier integrity, and expressions of inflammatory cytokines were analyzed. The results indicated that dietary supplementation with B. subtilis spores at concentrations of 1 × 106 CFU/g of feed (BS6 group) and 1 × 107 CFU/g of feed (BS7 group) improved body weight and body weight gain during d 0–42 (P < 0.05), while the feed intake of the BS7 group was highest (P < 0.05). Additionally, the BS6 group showed a better feed conversion ratio than the control (CON) group (P < 0.05). The BS7 group showed the lowest serum fluorescein isothiocyanate-dextran levels (P < 0.05), and both the BS6 and BS7 groups showed lower corticosterone levels than the CON group (P < 0.05). Additionally, both the BS6 and BS7 groups demonstrated increased villi height and villus height/crypt depth ratio, along with decreased crypt depth in the duodenum and ileum (P < 0.05). However, only the BS7 group exhibited greater improvements than the CON group in the jejunum at d 35. Furthermore, at d 14 and 35, mRNA expressions of occludin, claudin-1, and tight junction protein-1 in the jejunum were upregulated (P < 0.05), and expression levels of five inflammatory cytokine genes were downregulated in the ileum (P < 0.05). Our findings provide new insights and evidence supporting the application of B. subtilis 14823 for enhancing growth performance, gut barrier integrity, and modulating inflammatory cytokines in broilers.http://www.sciencedirect.com/science/article/pii/S0032579124010964B. subtilis 14823BroilersPerformanceGut barrier integrityInflammatory cytokines |
| spellingShingle | Sunisa Khongthong Pipat Piewngam Natthrit Roekngam Prapot Maliwan Sonsawan Kongpuckdee Juthatip Jeenkeawpleam Prawit Rodjan Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment Poultry Science B. subtilis 14823 Broilers Performance Gut barrier integrity Inflammatory cytokines |
| title | Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment |
| title_full | Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment |
| title_fullStr | Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment |
| title_full_unstemmed | Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment |
| title_short | Effects of dietary Bacillus subtilis 14823 on growth performance, gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment |
| title_sort | effects of dietary bacillus subtilis 14823 on growth performance gut barrier integrity and inflammatory response of broilers raised in a stressful tropical environment |
| topic | B. subtilis 14823 Broilers Performance Gut barrier integrity Inflammatory cytokines |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124010964 |
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