Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (I...

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Bibliographic Details
Main Authors: Qin Yu, Puthiyakunnon Santhosh, Zhang Yiduo, Wu Xianbo, Boddu Swapna, Luo Binde, Fan Hongying
Format: Article
Language:English
Published: Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences 2018-06-01
Series:Polish Journal of Food and Nutrition Sciences
Subjects:
immunomagnetic separation
LAMP
Escherichia coli
EHEC O157:H7
rfbE gene
ground beef
Online Access:http://www.degruyter.com/view/j/pjfns.2018.68.issue-2/pjfns-2017-0014/pjfns-2017-0014.xml?format=INT
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