Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease
Objective. Interleukin-17 (IL-17) is a cytokine that induces neutrophil recruitment and the release of inflammatory mediators in several inflammatory conditions; nevertheless, the involvement of IL-17 gene polymorphisms in chronic periodontitis (CP) has not been addressed yet. Our aim was to evaluat...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/846052 |
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| author | Jôice Dias Corrêa Mila Fernandes Moreira Madeira Renata Gonçalves Resende Jeane de Fátima Correia-Silva Ricardo Santiago Gomez Danielle da Glória de Souza Mauro Martins Teixeira Celso Martins Queiroz-Junior Tarcília Aparecida da Silva |
| author_facet | Jôice Dias Corrêa Mila Fernandes Moreira Madeira Renata Gonçalves Resende Jeane de Fátima Correia-Silva Ricardo Santiago Gomez Danielle da Glória de Souza Mauro Martins Teixeira Celso Martins Queiroz-Junior Tarcília Aparecida da Silva |
| author_sort | Jôice Dias Corrêa |
| collection | DOAJ |
| description | Objective. Interleukin-17 (IL-17) is a cytokine that induces neutrophil recruitment and the release of inflammatory mediators in several inflammatory conditions; nevertheless, the involvement of IL-17 gene polymorphisms in chronic periodontitis (CP) has not been addressed yet. Our aim was to evaluate the association between periodontal status and the polymorphisms IL-17A G197A and IL-17F C7488T in subjects with CP along with their impact on levels of inflammatory mediators. Material and Methods. Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). IL-17A G197A and IL-17F C7488T polymorphisms were determined using PCR-RFLP. Serum and periodontal tissues were collected and processed for ELISA, myeloperoxidase (MPO), and/or microscopic analysis. Results. The frequencies of genotypes in the CP group were significantly different from those of HC. Odds ratio indicated that increased risks for CP were associated with the -197A allele, not with the -7488T allele. In addition, the -197A allele was correlated with worse clinical parameters, higher MPO activity, and increased expression of inflammatory mediators (IL-17A and IL-8) than the other genotypes. Conclusions. These results indicate that the IL-17A -197A allele is associated with increased risk for CP, likely because this genotype relates to the enhanced inflammation in periodontal tissues. |
| format | Article |
| id | doaj-art-955c9db332f84e59a453abdc6f6336ab |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-955c9db332f84e59a453abdc6f6336ab2025-02-03T05:52:02ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/846052846052Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal DiseaseJôice Dias Corrêa0Mila Fernandes Moreira Madeira1Renata Gonçalves Resende2Jeane de Fátima Correia-Silva3Ricardo Santiago Gomez4Danielle da Glória de Souza5Mauro Martins Teixeira6Celso Martins Queiroz-Junior7Tarcília Aparecida da Silva8Departmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Microbiologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilDepartmento de Clínica, Patologia e Cirurgia Odontológicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, 31.270-901 Belo Horizonte, MG, BrazilObjective. Interleukin-17 (IL-17) is a cytokine that induces neutrophil recruitment and the release of inflammatory mediators in several inflammatory conditions; nevertheless, the involvement of IL-17 gene polymorphisms in chronic periodontitis (CP) has not been addressed yet. Our aim was to evaluate the association between periodontal status and the polymorphisms IL-17A G197A and IL-17F C7488T in subjects with CP along with their impact on levels of inflammatory mediators. Material and Methods. Genomic DNA was obtained from 30 CP patients and 30 healthy controls (HCs). IL-17A G197A and IL-17F C7488T polymorphisms were determined using PCR-RFLP. Serum and periodontal tissues were collected and processed for ELISA, myeloperoxidase (MPO), and/or microscopic analysis. Results. The frequencies of genotypes in the CP group were significantly different from those of HC. Odds ratio indicated that increased risks for CP were associated with the -197A allele, not with the -7488T allele. In addition, the -197A allele was correlated with worse clinical parameters, higher MPO activity, and increased expression of inflammatory mediators (IL-17A and IL-8) than the other genotypes. Conclusions. These results indicate that the IL-17A -197A allele is associated with increased risk for CP, likely because this genotype relates to the enhanced inflammation in periodontal tissues.http://dx.doi.org/10.1155/2012/846052 |
| spellingShingle | Jôice Dias Corrêa Mila Fernandes Moreira Madeira Renata Gonçalves Resende Jeane de Fátima Correia-Silva Ricardo Santiago Gomez Danielle da Glória de Souza Mauro Martins Teixeira Celso Martins Queiroz-Junior Tarcília Aparecida da Silva Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease Mediators of Inflammation |
| title | Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease |
| title_full | Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease |
| title_fullStr | Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease |
| title_full_unstemmed | Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease |
| title_short | Association between Polymorphisms in Interleukin-17A and -17F Genes and Chronic Periodontal Disease |
| title_sort | association between polymorphisms in interleukin 17a and 17f genes and chronic periodontal disease |
| url | http://dx.doi.org/10.1155/2012/846052 |
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