Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine
The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/850969 |
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| author | Maysa F. Zampa Inês Maria de S. Araújo José Ribeiro dos Santos Júnior Valtencir Zucolotto José Roberto de S. A. Leite Carla Eiras |
| author_facet | Maysa F. Zampa Inês Maria de S. Araújo José Ribeiro dos Santos Júnior Valtencir Zucolotto José Roberto de S. A. Leite Carla Eiras |
| author_sort | Maysa F. Zampa |
| collection | DOAJ |
| description | The antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries. |
| format | Article |
| id | doaj-art-9556921e2c294b2788266bc40bc37433 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-9556921e2c294b2788266bc40bc374332025-02-03T01:31:30ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792012-01-01201210.1155/2012/850969850969Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of DopamineMaysa F. Zampa0Inês Maria de S. Araújo1José Ribeiro dos Santos Júnior2Valtencir Zucolotto3José Roberto de S. A. Leite4Carla Eiras5Instituto Federal de Educação, Ciência e Tecnologia do Piauí (IFPI), Campus Parnaíba, 64210260 Parnaíba, PI, BrazilBiotec, Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Universidade Federal do Piauí (UFPI), Campus Ministro Reis Velloso (CMRV), 64202020 Parnaíba, PI, BrazilDepartamento de Química, Centro de Ciências da Natureza (CCN), Universidade Federal do Piauí (UFPI), 64049550 Teresina, PI, BrazilGrupo de Biofísica Molecular Sérgio Mascarenhas, Instituto de Física de São Carlos (IFSC), USP, 13560970 São Carlos, SP, BrazilBiotec, Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Universidade Federal do Piauí (UFPI), Campus Ministro Reis Velloso (CMRV), 64202020 Parnaíba, PI, BrazilBiotec, Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Universidade Federal do Piauí (UFPI), Campus Ministro Reis Velloso (CMRV), 64202020 Parnaíba, PI, BrazilThe antimicrobial peptide dermaseptin 01 (DS 01), from the skin secretion of Phyllomedusa hypochondrialis frogs, was immobilized in nanostructured layered films in conjunction with nickel tetrasulfonated phthalocyanines (NiTsPc), widely used in electronic devices, using layer-by-layer technique. The films were used as a biosensor to detect the presence of dopamine (DA), a neurotransmitter associated with diseases such as Alzheimer's and Parkinson's, with detection limits in the order of 10−6 mol L−1. The use of DS 01 in LbL film generated selectivity in the detection of DA despite the presence of ascorbic acid found in biological fluids. This work is the first to report that the antimicrobial peptide and NiTsPc LbL film exhibits electroanalytical activity to DA oxidation. The selectivity in the detection of DA is a fundamental aspect for the development of electrochemical sensors with potential applications in the biomedical and pharmaceutical industries.http://dx.doi.org/10.1155/2012/850969 |
| spellingShingle | Maysa F. Zampa Inês Maria de S. Araújo José Ribeiro dos Santos Júnior Valtencir Zucolotto José Roberto de S. A. Leite Carla Eiras Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine International Journal of Analytical Chemistry |
| title | Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine |
| title_full | Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine |
| title_fullStr | Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine |
| title_full_unstemmed | Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine |
| title_short | Development of a Novel Biosensor Using Cationic Antimicrobial Peptide and Nickel Phthalocyanine Ultrathin Films for Electrochemical Detection of Dopamine |
| title_sort | development of a novel biosensor using cationic antimicrobial peptide and nickel phthalocyanine ultrathin films for electrochemical detection of dopamine |
| url | http://dx.doi.org/10.1155/2012/850969 |
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