Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method
The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/408306 |
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| author | Sreeramanan Subramaniam Jeevandran Sundarasekar Geethaa Sahgal Vikneswaran Murugaiyah |
| author_facet | Sreeramanan Subramaniam Jeevandran Sundarasekar Geethaa Sahgal Vikneswaran Murugaiyah |
| author_sort | Sreeramanan Subramaniam |
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| description | The Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples of H. littoralis. The separation was achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract (2.54±0.02 μg/mg) whereas the least was in the root extract (0.71±0.02 μg/mg) of the wild plants. Few callus culture samples had high content of lycorine, comparable to that of wild plants. The results showed that plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.5 μM (2.58±0.38 μg/mg) or a combination of 2,4-D at 9.00 μM with 4.5 μM of 6-benzylaminopurine (BAP), were the optimum concentrations for the production of high lycorine (2.45±0.15 μg/mg) content in callus culture. The present analytical method could be of value for routine quantification of lycorine in the tissue culture production and standardization of the raw material or extracts of H. littoralis. |
| format | Article |
| id | doaj-art-95453a70f2d441a984dbd5d629e05723 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-95453a70f2d441a984dbd5d629e057232025-02-03T05:59:14ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/408306408306Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV MethodSreeramanan Subramaniam0Jeevandran Sundarasekar1Geethaa Sahgal2Vikneswaran Murugaiyah3School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, MalaysiaInstitute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Penang, MalaysiaSchool of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800 Penang, MalaysiaThe Hymenocallis littoralis, an ornamental and medicinal plant, had been traditionally used for wound healing. In the present study, an analytical method using HPLC with ultraviolet detection was developed for the quantification of lycorine in the extracts of different parts of wild plant and tissue culture samples of H. littoralis. The separation was achieved using a reversed-phase column. The method was found to be accurate, repeatable, and sensitive for the quantification of minute amount of lycorine present in the samples. The highest lycorine content was found in the bulb extract (2.54±0.02 μg/mg) whereas the least was in the root extract (0.71±0.02 μg/mg) of the wild plants. Few callus culture samples had high content of lycorine, comparable to that of wild plants. The results showed that plant growth regulators, 2,4-dichlorophenoxyacetic acid (2,4-D) alone at 4.5 μM (2.58±0.38 μg/mg) or a combination of 2,4-D at 9.00 μM with 4.5 μM of 6-benzylaminopurine (BAP), were the optimum concentrations for the production of high lycorine (2.45±0.15 μg/mg) content in callus culture. The present analytical method could be of value for routine quantification of lycorine in the tissue culture production and standardization of the raw material or extracts of H. littoralis.http://dx.doi.org/10.1155/2014/408306 |
| spellingShingle | Sreeramanan Subramaniam Jeevandran Sundarasekar Geethaa Sahgal Vikneswaran Murugaiyah Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method The Scientific World Journal |
| title | Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method |
| title_full | Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method |
| title_fullStr | Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method |
| title_full_unstemmed | Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method |
| title_short | Comparative Analysis of Lycorine in Wild Plant and Callus Culture Samples of Hymenocallis littoralis by HPLC-UV Method |
| title_sort | comparative analysis of lycorine in wild plant and callus culture samples of hymenocallis littoralis by hplc uv method |
| url | http://dx.doi.org/10.1155/2014/408306 |
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