PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression
Quorum sensing (QS) system can dynamically control the expression of proteins along with the cell growth. The promoting period of QS system has been little focused on until now. In this study, a self-induced dynamic regulated expression (SIDRE) system was constructed in Escherichia coli. To enable t...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000484 |
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| author | Zhuoning Cao Zhen Liu Guilin Zhang Xiangzhao Mao |
| author_facet | Zhuoning Cao Zhen Liu Guilin Zhang Xiangzhao Mao |
| author_sort | Zhuoning Cao |
| collection | DOAJ |
| description | Quorum sensing (QS) system can dynamically control the expression of proteins along with the cell growth. The promoting period of QS system has been little focused on until now. In this study, a self-induced dynamic regulated expression (SIDRE) system was constructed in Escherichia coli. To enable the system suitable for the expression of enzymes, promoter engineering was used to obtain PluxI mutants. To test the SIDRE system, alginate lyase AL493 and esterase Est7 were used as target protein for expression. The enzyme activity of alginate lyase and esterase reached 96.38 % and 106.71 % of the control strains containing the T7 promoter. In high-density fermentation, the activity of alginate lyase expressed by the SIDRE system with PluxI(T-38C) as promoter was 4.34-fold of that expressed by the T7 promoter. Therefore, the PluxI mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression. |
| format | Article |
| id | doaj-art-953cdea57e5c456bb99bdfcac3fe3649 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-953cdea57e5c456bb99bdfcac3fe36492025-02-03T00:27:37ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112518411849PluxI mutants with different promoting period and their application for quorum sensing regulated protein expressionZhuoning Cao0Zhen Liu1Guilin Zhang2Xiangzhao Mao3College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Corresponding authors at: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.College of Food Science and Engineering, Ocean University of China, Qingdao 266003, ChinaCollege of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China; Corresponding authors at: College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.Quorum sensing (QS) system can dynamically control the expression of proteins along with the cell growth. The promoting period of QS system has been little focused on until now. In this study, a self-induced dynamic regulated expression (SIDRE) system was constructed in Escherichia coli. To enable the system suitable for the expression of enzymes, promoter engineering was used to obtain PluxI mutants. To test the SIDRE system, alginate lyase AL493 and esterase Est7 were used as target protein for expression. The enzyme activity of alginate lyase and esterase reached 96.38 % and 106.71 % of the control strains containing the T7 promoter. In high-density fermentation, the activity of alginate lyase expressed by the SIDRE system with PluxI(T-38C) as promoter was 4.34-fold of that expressed by the T7 promoter. Therefore, the PluxI mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.http://www.sciencedirect.com/science/article/pii/S2213453023000484Quorum sensingPromoter engineeringEnzymesAlginate lyaseEsteraseHigh-density fermentation |
| spellingShingle | Zhuoning Cao Zhen Liu Guilin Zhang Xiangzhao Mao PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression Food Science and Human Wellness Quorum sensing Promoter engineering Enzymes Alginate lyase Esterase High-density fermentation |
| title | PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression |
| title_full | PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression |
| title_fullStr | PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression |
| title_full_unstemmed | PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression |
| title_short | PluxI mutants with different promoting period and their application for quorum sensing regulated protein expression |
| title_sort | pluxi mutants with different promoting period and their application for quorum sensing regulated protein expression |
| topic | Quorum sensing Promoter engineering Enzymes Alginate lyase Esterase High-density fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000484 |
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