Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs
Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce ra...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/861794 |
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| author | Jennifer Mumaw Erin T. Jordan Corinne Sonnet Ronke M. Olabisi Elizabeth A. Olmsted-Davis Alan R. Davis John F. Peroni Jennifer L. West Franklin West Yangqing Lu Steven L. Stice |
| author_facet | Jennifer Mumaw Erin T. Jordan Corinne Sonnet Ronke M. Olabisi Elizabeth A. Olmsted-Davis Alan R. Davis John F. Peroni Jennifer L. West Franklin West Yangqing Lu Steven L. Stice |
| author_sort | Jennifer Mumaw |
| collection | DOAJ |
| description | Autologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation. However, methods to make these treatments available for clinical applications are presently lacking. In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities. MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion. We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation. Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy. These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs. |
| format | Article |
| id | doaj-art-951b1b0336bc47668a0794865966a47c |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-951b1b0336bc47668a0794865966a47c2025-02-03T06:01:32ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/861794861794Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCsJennifer Mumaw0Erin T. Jordan1Corinne Sonnet2Ronke M. Olabisi3Elizabeth A. Olmsted-Davis4Alan R. Davis5John F. Peroni6Jennifer L. West7Franklin West8Yangqing Lu9Steven L. Stice10Department of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA 30602, USADepartment of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA 30602, USACenter for Cell & Gene Therapy, Baylor College of Medicine, One Baylor Plaza, Room N1010, Houston, TX 77030, USADepartment of Molecular Medicine, City of Hope Beckman Research Institute, 1500 East Duarte Road, Duarte, CA 91010, USACenter for Cell & Gene Therapy, Baylor College of Medicine, One Baylor Plaza, Room N1010, Houston, TX 77030, USACenter for Cell & Gene Therapy, Baylor College of Medicine, One Baylor Plaza, Room N1010, Houston, TX 77030, USADepartment of Large Animal Medicine, College of Veterinary Medicine, University of Georgia H-322, 501 D.W. Brooks Drive, Athens, GA 30602-7385, USADepartment of Bioengineering, Rice University, 6100 Main Street, Houston, TX 77005-1892, USADepartment of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA 30602, USADepartment of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA 30602, USADepartment of Animal and Dairy Science, Regenerative Bioscience Center, University of Georgia, 425 River Road, Athens, GA 30602, USAAutologous bone grafting is the most effective treatment for long-bone nonunions, but it poses considerable risks to donors, necessitating the development of alternative therapeutics. Poly(ethylene glycol) (PEG) microencapsulation and BMP2 transgene delivery are being developed together to induce rapid bone formation. However, methods to make these treatments available for clinical applications are presently lacking. In this study we used mesenchymal stem cells (MSCs) due to their ease of harvest, replication potential, and immunomodulatory capabilities. MSCs were from sheep and pig due to their appeal as large animal models for bone nonunion. We demonstrated that cryopreservation of these microencapsulated MSCs did not affect their cell viability, adenoviral BMP2 production, or ability to initiate bone formation. Additionally, microspheres showed no appreciable damage from cryopreservation when examined with light and electron microscopy. These results validate the use of cryopreservation in preserving the viability and functionality of PEG-encapsulated BMP2-transduced MSCs.http://dx.doi.org/10.1155/2012/861794 |
| spellingShingle | Jennifer Mumaw Erin T. Jordan Corinne Sonnet Ronke M. Olabisi Elizabeth A. Olmsted-Davis Alan R. Davis John F. Peroni Jennifer L. West Franklin West Yangqing Lu Steven L. Stice Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs International Journal of Biomaterials |
| title | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
| title_full | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
| title_fullStr | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
| title_full_unstemmed | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
| title_short | Rapid Heterotrophic Ossification with Cryopreserved Poly(ethylene glycol-) Microencapsulated BMP2-Expressing MSCs |
| title_sort | rapid heterotrophic ossification with cryopreserved poly ethylene glycol microencapsulated bmp2 expressing mscs |
| url | http://dx.doi.org/10.1155/2012/861794 |
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