Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response
Regulatory T (Tregs) cells play an important role in mediating tolerance to self-antigens but can also mediate detrimental tolerance to tumours and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome- (BAC-) transgenic DEpletion o...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/1450398 |
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| author | Junhua Wang Myriam Siffert Markus Spiliotis Bruno Gottstein |
| author_facet | Junhua Wang Myriam Siffert Markus Spiliotis Bruno Gottstein |
| author_sort | Junhua Wang |
| collection | DOAJ |
| description | Regulatory T (Tregs) cells play an important role in mediating tolerance to self-antigens but can also mediate detrimental tolerance to tumours and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome- (BAC-) transgenic DEpletion of REGulatory T cells (DEREG) mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3+ Tregs selectively express enhanced green fluorescent protein (eGFP) and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about an important consideration that long-term administration of DT induces a humoral immune response with an appropriate production of anti-DT antibodies that can inactivate DT and thus abrogate its effect in the DEREG mouse. Additionally, we showed that anti-DT mouse serum partially neutralized DT-induced Foxp3 inhibition. |
| format | Article |
| id | doaj-art-94f46cdbcdbc47feafae8da7a91228d0 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-94f46cdbcdbc47feafae8da7a91228d02025-02-03T01:01:11ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/14503981450398Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody ResponseJunhua Wang0Myriam Siffert1Markus Spiliotis2Bruno Gottstein3Institute of Parasitology, University of Bern, Bern, SwitzerlandDepartment of Infectious Diseases and Pathobiology, University of Bern, Bern, SwitzerlandInstitute of Parasitology, University of Bern, Bern, SwitzerlandInstitute of Parasitology, University of Bern, Bern, SwitzerlandRegulatory T (Tregs) cells play an important role in mediating tolerance to self-antigens but can also mediate detrimental tolerance to tumours and pathogens in a Foxp3-dependent manner. Genetic tools exploiting the foxp3 locus including bacterial artificial chromosome- (BAC-) transgenic DEpletion of REGulatory T cells (DEREG) mice have provided essential information on Treg biology and the potential therapeutic modulation of tolerance. In DEREG mice, Foxp3+ Tregs selectively express enhanced green fluorescent protein (eGFP) and diphtheria toxin (DT) receptor, allowing for the specific depletion of Tregs through DT administration. We here provide a detailed overview about an important consideration that long-term administration of DT induces a humoral immune response with an appropriate production of anti-DT antibodies that can inactivate DT and thus abrogate its effect in the DEREG mouse. Additionally, we showed that anti-DT mouse serum partially neutralized DT-induced Foxp3 inhibition.http://dx.doi.org/10.1155/2016/1450398 |
| spellingShingle | Junhua Wang Myriam Siffert Markus Spiliotis Bruno Gottstein Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response Journal of Immunology Research |
| title | Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response |
| title_full | Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response |
| title_fullStr | Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response |
| title_full_unstemmed | Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response |
| title_short | Repeated Long-Term DT Application in the DEREG Mouse Induces a Neutralizing Anti-DT Antibody Response |
| title_sort | repeated long term dt application in the dereg mouse induces a neutralizing anti dt antibody response |
| url | http://dx.doi.org/10.1155/2016/1450398 |
| work_keys_str_mv | AT junhuawang repeatedlongtermdtapplicationinthederegmouseinducesaneutralizingantidtantibodyresponse AT myriamsiffert repeatedlongtermdtapplicationinthederegmouseinducesaneutralizingantidtantibodyresponse AT markusspiliotis repeatedlongtermdtapplicationinthederegmouseinducesaneutralizingantidtantibodyresponse AT brunogottstein repeatedlongtermdtapplicationinthederegmouseinducesaneutralizingantidtantibodyresponse |