The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1
Abstract Background To investigate the mechanism by which Acinetobacter lwoffii-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN). Methods After establishing a co-culture model of Acinetobacter lwoffii and ECT1/E6E7 cells,...
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BMC
2025-05-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-025-04043-y |
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| author | Jiayuan Du Tianmeng Tan Yaojie Yu He Wang |
| author_facet | Jiayuan Du Tianmeng Tan Yaojie Yu He Wang |
| author_sort | Jiayuan Du |
| collection | DOAJ |
| description | Abstract Background To investigate the mechanism by which Acinetobacter lwoffii-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN). Methods After establishing a co-culture model of Acinetobacter lwoffii and ECT1/E6E7 cells, the proliferation, migration and apoptosis of ECT1/E6E7 cells were detected by CCK8, transwell and flow cytometry assays. The relative expression levels of circ-LDHA and HMGB1 were detected by real-time quantitative PCR (qRT-PCR). Results Circ-LDHA expression was lower in normal cervical tissues and greater in the cervical cancer group and CIN group than in the normal group (P < 0.05). The optimal coculture model with Acinetobacter lwoffii was an MOI of 100 following 18 h-24 h of coculture. The proliferative activity of the ECT1/E6E7 cells and the relative mRNA expression levels of circ-LDHA and HMGB1 were significantly increased within 36h of coculture, and the apoptosis rate of the ECT1/E6E7 cells was significantly increased after 12 h of coculture. The migration rate of the ECT1/E6E7 cells increased from 12 h to 24 h (all P < 0.05). Conclusion Acinetobacter lwoffii can regulate the development and progression of CIN by regulating the expression of circ-LDHA and HMGB1 in a time-dependent manner. |
| format | Article |
| id | doaj-art-94e4a30f8ddb4d9fba463f4384e6fa1e |
| institution | DOAJ |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj-art-94e4a30f8ddb4d9fba463f4384e6fa1e2025-08-20T03:16:50ZengBMCBMC Microbiology1471-21802025-05-0125111210.1186/s12866-025-04043-yThe mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1Jiayuan Du0Tianmeng Tan1Yaojie Yu2He Wang3Department of Gynecology Oncology, Guangxi Medical University Cancer HospitalDepartment of Gynecology Oncology, Guangxi Medical University Cancer HospitalDepartment of Gynecology Oncology, Guangxi Medical University Cancer HospitalDepartment of Gynecology Oncology, Guangxi Medical University Cancer HospitalAbstract Background To investigate the mechanism by which Acinetobacter lwoffii-induced circ-LDHA/HMGB1 activation regulates the ability of ECT1/E6E7 cells to promote cervical intraepithelial neoplasia (CIN). Methods After establishing a co-culture model of Acinetobacter lwoffii and ECT1/E6E7 cells, the proliferation, migration and apoptosis of ECT1/E6E7 cells were detected by CCK8, transwell and flow cytometry assays. The relative expression levels of circ-LDHA and HMGB1 were detected by real-time quantitative PCR (qRT-PCR). Results Circ-LDHA expression was lower in normal cervical tissues and greater in the cervical cancer group and CIN group than in the normal group (P < 0.05). The optimal coculture model with Acinetobacter lwoffii was an MOI of 100 following 18 h-24 h of coculture. The proliferative activity of the ECT1/E6E7 cells and the relative mRNA expression levels of circ-LDHA and HMGB1 were significantly increased within 36h of coculture, and the apoptosis rate of the ECT1/E6E7 cells was significantly increased after 12 h of coculture. The migration rate of the ECT1/E6E7 cells increased from 12 h to 24 h (all P < 0.05). Conclusion Acinetobacter lwoffii can regulate the development and progression of CIN by regulating the expression of circ-LDHA and HMGB1 in a time-dependent manner.https://doi.org/10.1186/s12866-025-04043-yAcinetobacter lwoffiiCervical intraepithelial neoplasiaCocultureCirc-LDHAHMGB1 |
| spellingShingle | Jiayuan Du Tianmeng Tan Yaojie Yu He Wang The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1 BMC Microbiology Acinetobacter lwoffii Cervical intraepithelial neoplasia Coculture Circ-LDHA HMGB1 |
| title | The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1 |
| title_full | The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1 |
| title_fullStr | The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1 |
| title_full_unstemmed | The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1 |
| title_short | The mechanism of ECT1/E6E7 cervical intraepithelial neoplasia cells regulated by Acinetobacter lwoffii through circ-LDHA/HMGB1 |
| title_sort | mechanism of ect1 e6e7 cervical intraepithelial neoplasia cells regulated by acinetobacter lwoffii through circ ldha hmgb1 |
| topic | Acinetobacter lwoffii Cervical intraepithelial neoplasia Coculture Circ-LDHA HMGB1 |
| url | https://doi.org/10.1186/s12866-025-04043-y |
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