<i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation
Solar ultraviolet radiation (UV) may cause DNA damage. We first report in this study that the large subunit ribosome protein RPL12, from <i>Bomby mori</i> (BmRPL12), participated in UV-induced DNA damage repair. BmRPL12 enhanced the resistance of <i>Escherichia coli</i> (<...
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MDPI AG
2025-02-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/16/2/187 |
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| author | Qi Tang Ceru Chen Jiaying Huang Guohui Li Feifei Zhu Qian Yu Lindan Sun Huiqing Chen Liang Chen Shangshang Ma Xiaoyong Liu Keping Chen |
| author_facet | Qi Tang Ceru Chen Jiaying Huang Guohui Li Feifei Zhu Qian Yu Lindan Sun Huiqing Chen Liang Chen Shangshang Ma Xiaoyong Liu Keping Chen |
| author_sort | Qi Tang |
| collection | DOAJ |
| description | Solar ultraviolet radiation (UV) may cause DNA damage. We first report in this study that the large subunit ribosome protein RPL12, from <i>Bomby mori</i> (BmRPL12), participated in UV-induced DNA damage repair. BmRPL12 enhanced the resistance of <i>Escherichia coli</i> (<i>E. coli</i>) to UV radiation and facilitated faster repair of UV-induced DNA damage in silkworm cells. BmRPL12 mainly existed in the cytoplasm in the dimer forms, and the N-terminal nuclear export signal was crucial for the localization of BmRPL12. After UV radiation, BmRPL12 was unable to localize at the UV-induced DNA damage sites to participate in damage repair directly and might indirectly regulate UV-induced DNA damage repair. Our previous research found that BmNPV Bm65 was an important UV damage-induced endonuclease. Here, it was further found that in BmNPV-infected silkworm cells, BmRPL12 in monomeric forms interacted with the virus Bm65 protein only after UV radiation, and BmRPL12 specifically localized at the UV-induced DNA damage sites only in the presence of Bm65. We speculate that after viral infection in cells subjected to UV-induced DNA damage, viral protein Bm65 interacts with BmRPL12 and localizes BmRPL12 to sites of UV-damaged DNA. |
| format | Article |
| id | doaj-art-94de8e4c7fa74ad1aafe092251dd8104 |
| institution | DOAJ |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-94de8e4c7fa74ad1aafe092251dd81042025-08-20T02:44:46ZengMDPI AGInsects2075-44502025-02-0116218710.3390/insects16020187<i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet RadiationQi Tang0Ceru Chen1Jiaying Huang2Guohui Li3Feifei Zhu4Qian Yu5Lindan Sun6Huiqing Chen7Liang Chen8Shangshang Ma9Xiaoyong Liu10Keping Chen11School of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSchool of Life Sciences, Jiangsu University, 301# Xuefu Road, Zhenjiang 212013, ChinaSolar ultraviolet radiation (UV) may cause DNA damage. We first report in this study that the large subunit ribosome protein RPL12, from <i>Bomby mori</i> (BmRPL12), participated in UV-induced DNA damage repair. BmRPL12 enhanced the resistance of <i>Escherichia coli</i> (<i>E. coli</i>) to UV radiation and facilitated faster repair of UV-induced DNA damage in silkworm cells. BmRPL12 mainly existed in the cytoplasm in the dimer forms, and the N-terminal nuclear export signal was crucial for the localization of BmRPL12. After UV radiation, BmRPL12 was unable to localize at the UV-induced DNA damage sites to participate in damage repair directly and might indirectly regulate UV-induced DNA damage repair. Our previous research found that BmNPV Bm65 was an important UV damage-induced endonuclease. Here, it was further found that in BmNPV-infected silkworm cells, BmRPL12 in monomeric forms interacted with the virus Bm65 protein only after UV radiation, and BmRPL12 specifically localized at the UV-induced DNA damage sites only in the presence of Bm65. We speculate that after viral infection in cells subjected to UV-induced DNA damage, viral protein Bm65 interacts with BmRPL12 and localizes BmRPL12 to sites of UV-damaged DNA.https://www.mdpi.com/2075-4450/16/2/187<i>Bombyx mori</i>BmNPVultraviolet radiationDNA damage repairprotein-protein interaction |
| spellingShingle | Qi Tang Ceru Chen Jiaying Huang Guohui Li Feifei Zhu Qian Yu Lindan Sun Huiqing Chen Liang Chen Shangshang Ma Xiaoyong Liu Keping Chen <i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation Insects <i>Bombyx mori</i> BmNPV ultraviolet radiation DNA damage repair protein-protein interaction |
| title | <i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation |
| title_full | <i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation |
| title_fullStr | <i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation |
| title_full_unstemmed | <i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation |
| title_short | <i>Bombyx mori</i> RPL12 Participates in UV-Induced DNA Damage Repair and Interacts with BmNPV Bm65 Protein Only After Ultraviolet Radiation |
| title_sort | i bombyx mori i rpl12 participates in uv induced dna damage repair and interacts with bmnpv bm65 protein only after ultraviolet radiation |
| topic | <i>Bombyx mori</i> BmNPV ultraviolet radiation DNA damage repair protein-protein interaction |
| url | https://www.mdpi.com/2075-4450/16/2/187 |
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