Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses. The binary vector pBINUbiGUSint, which contained neomycin phosphotransferase II (nptII) as a selectable marker gene and β-glucuronidase (uidA)...
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Main Authors: | José M. Alvarez, Ricardo J. Ordás |
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Format: | Article |
Language: | English |
Published: |
Wiley
2013-01-01
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Series: | The Scientific World Journal |
Online Access: | http://dx.doi.org/10.1155/2013/681792 |
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