Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection

An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses. The binary vector pBINUbiGUSint, which contained neomycin phosphotransferase II (nptII) as a selectable marker gene and β-glucuronidase (uidA)...

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Bibliographic Details
Main Authors: José M. Alvarez, Ricardo J. Ordás
Format: Article
Language:English
Published: Wiley 2013-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1155/2013/681792
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