A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4

A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4

Abstract The soilborne pathogen Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is currently devastating banana production worldwide. Once introduced, it is not possible to eradicate the pathogen from soils where it can survive for decades. The only management option available then is to...

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Bibliographic Details
Main Authors: Megan Ceris Matthews, Jos van der Linden, Isabelle Robène, Samuel Rozsasi, Beatrix Coetzee, Manuela Campa, Johan Burger, Uzoma Nobel Akwuruoha, Ndubuisi Johnkennedy Madufor, Willem Perold, Umezuruike Linus Opara, Altus Viljoen, Diane Mostert
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
Fusarium oxysporum f. sp. cubense tropical race 4
CRISPR/Cas12a
Banana Fusarium wilt
Disease diagnosis
Rapid detection
Online Access:https://doi.org/10.1038/s41598-025-85633-8
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