Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity
BackgroundIt is well-documented that house dust mite (HDM) exposure can cause tissue damage and activate innate immune responses. However, its role in promoting gastrointestinal sensitization and allergenicity to food proteins has been relatively unexplored.MethodsThis study investigates the immunos...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1505003/full |
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| author | Sara Benedé Leticia Pérez-Rodríguez David Menchén-Martínez Elena Molina Rosina López-Fandiño |
| author_facet | Sara Benedé Leticia Pérez-Rodríguez David Menchén-Martínez Elena Molina Rosina López-Fandiño |
| author_sort | Sara Benedé |
| collection | DOAJ |
| description | BackgroundIt is well-documented that house dust mite (HDM) exposure can cause tissue damage and activate innate immune responses. However, its role in promoting gastrointestinal sensitization and allergenicity to food proteins has been relatively unexplored.MethodsThis study investigates the immunostimulatory effects of HDM in a murine model of oral sensitization to egg white (EW) in the absence of exogenous adjuvants. Additionally, we examined a proteolytically inactivated form of HDM (iHDM) to assess the contribution of HDM protease activity to its adjuvant potential.ResultsBoth HDM and iHDM enhanced allergic responses to EW proteins via the oral route, evidenced by mast cell degranulation in the intestinal tract upon EW challenge. Notably, only iHDM induced detectable concentrations of serum EW-specific IgE and IgG1 antibodies. Whereas HDM increased intestinal expression of genes encoding tight junction proteins and Th2-inducing alarmins to a greater extent than iHDM, active proteinases were not required for its adjuvant activity, as iHDM preferentially promoted Th2 responses in intestinal lymphoid tissues.ConclusionsThese findings suggest that ingestion of environmental dust may contribute to food allergy development and highlight the complex and context-dependent nature of the adjuvant activity of HDM. |
| format | Article |
| id | doaj-art-9479c63e48184ffdaffb2845eda56bcf |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-9479c63e48184ffdaffb2845eda56bcf2025-01-20T05:23:50ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-01-011510.3389/fimmu.2024.15050031505003Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activitySara BenedéLeticia Pérez-RodríguezDavid Menchén-MartínezElena MolinaRosina López-FandiñoBackgroundIt is well-documented that house dust mite (HDM) exposure can cause tissue damage and activate innate immune responses. However, its role in promoting gastrointestinal sensitization and allergenicity to food proteins has been relatively unexplored.MethodsThis study investigates the immunostimulatory effects of HDM in a murine model of oral sensitization to egg white (EW) in the absence of exogenous adjuvants. Additionally, we examined a proteolytically inactivated form of HDM (iHDM) to assess the contribution of HDM protease activity to its adjuvant potential.ResultsBoth HDM and iHDM enhanced allergic responses to EW proteins via the oral route, evidenced by mast cell degranulation in the intestinal tract upon EW challenge. Notably, only iHDM induced detectable concentrations of serum EW-specific IgE and IgG1 antibodies. Whereas HDM increased intestinal expression of genes encoding tight junction proteins and Th2-inducing alarmins to a greater extent than iHDM, active proteinases were not required for its adjuvant activity, as iHDM preferentially promoted Th2 responses in intestinal lymphoid tissues.ConclusionsThese findings suggest that ingestion of environmental dust may contribute to food allergy development and highlight the complex and context-dependent nature of the adjuvant activity of HDM.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1505003/fullfood allergyhouse dust miteproteinase activityegg whiteoral sensitization |
| spellingShingle | Sara Benedé Leticia Pérez-Rodríguez David Menchén-Martínez Elena Molina Rosina López-Fandiño Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity Frontiers in Immunology food allergy house dust mite proteinase activity egg white oral sensitization |
| title | Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity |
| title_full | Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity |
| title_fullStr | Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity |
| title_full_unstemmed | Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity |
| title_short | Ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity |
| title_sort | ingested house dust mite favors sensitization to egg white in mice independently of its proteinase activity |
| topic | food allergy house dust mite proteinase activity egg white oral sensitization |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1505003/full |
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