HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization
Background: Assessment of HER-2/neu status in invasive breast cancer is crucial to establish eligibility for trastuzumab and taxane based chemotherapy. Next to immunohistochemistry (IHC) to evaluate protein overexpression, a second line gene amplification test is required for cases with equivocal pr...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2009-01-01
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| Series: | Cellular Oncology |
| Online Access: | http://dx.doi.org/10.3233/CLO-2009-0461 |
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| _version_ | 1832566627015589888 |
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| author | Cathy B. Moelans Roel A. de Weger Marja T.M. van Blokland Chantal Ezendam Sabrina Elshof Marcel G. J. Tilanus Paul J. van Diest |
| author_facet | Cathy B. Moelans Roel A. de Weger Marja T.M. van Blokland Chantal Ezendam Sabrina Elshof Marcel G. J. Tilanus Paul J. van Diest |
| author_sort | Cathy B. Moelans |
| collection | DOAJ |
| description | Background: Assessment of HER-2/neu status in invasive breast cancer is crucial to establish eligibility for trastuzumab and taxane based chemotherapy. Next to immunohistochemistry (IHC) to evaluate protein overexpression, a second line gene amplification test is required for cases with equivocal protein expression. This study aimed to validate a new PCR based test, called Multiplex Ligation-dependent Probe Amplification (MLPA), as a simple and quick method to assess HER-2/neu gene amplification status in invasive breast cancer. |
| format | Article |
| id | doaj-art-94711039197844a190f01ba1b7778195 |
| institution | Kabale University |
| issn | 1570-5870 1875-8606 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cellular Oncology |
| spelling | doaj-art-94711039197844a190f01ba1b77781952025-02-03T01:03:38ZengWileyCellular Oncology1570-58701875-86062009-01-0131111010.3233/CLO-2009-0461HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ HybridizationCathy B. Moelans0Roel A. de Weger1Marja T.M. van Blokland2Chantal Ezendam3Sabrina Elshof4Marcel G. J. Tilanus5Paul J. van Diest6Department of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsDepartment of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsDepartment of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsDepartment of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsDepartment of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsDepartment of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsDepartment of Pathology, University Medical Centre Utrecht, Utrecht, The NetherlandsBackground: Assessment of HER-2/neu status in invasive breast cancer is crucial to establish eligibility for trastuzumab and taxane based chemotherapy. Next to immunohistochemistry (IHC) to evaluate protein overexpression, a second line gene amplification test is required for cases with equivocal protein expression. This study aimed to validate a new PCR based test, called Multiplex Ligation-dependent Probe Amplification (MLPA), as a simple and quick method to assess HER-2/neu gene amplification status in invasive breast cancer.http://dx.doi.org/10.3233/CLO-2009-0461 |
| spellingShingle | Cathy B. Moelans Roel A. de Weger Marja T.M. van Blokland Chantal Ezendam Sabrina Elshof Marcel G. J. Tilanus Paul J. van Diest HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization Cellular Oncology |
| title | HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization |
| title_full | HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization |
| title_fullStr | HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization |
| title_full_unstemmed | HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization |
| title_short | HER-2/neu Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification in Comparison with Immunohistochemistry and In Situ Hybridization |
| title_sort | her 2 neu amplification testing in breast cancer by multiplex ligation dependent probe amplification in comparison with immunohistochemistry and in situ hybridization |
| url | http://dx.doi.org/10.3233/CLO-2009-0461 |
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