Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags
Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety. In this study, 5 species of bacteria, mainly gram-positive bacteria including three species of Bacillus spp., Arthrobacter arilaitensis and Staphylococcus warneri, were isolated and iden...
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| Format: | Article |
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Tsinghua University Press
2022-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001021 |
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| author | Shen Yang Zijin Yuan Jude Juventus Aweya Shanggui Deng Wuyin Weng Yueling Zhang Guangming Liu |
| author_facet | Shen Yang Zijin Yuan Jude Juventus Aweya Shanggui Deng Wuyin Weng Yueling Zhang Guangming Liu |
| author_sort | Shen Yang |
| collection | DOAJ |
| description | Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety. In this study, 5 species of bacteria, mainly gram-positive bacteria including three species of Bacillus spp., Arthrobacter arilaitensis and Staphylococcus warneri, were isolated and identified from bacteria-contaminated A. auricular culture bags. An in silico predicted antimicrobial peptide from the β-1,3-glucan-binding protein sequence of Penaeus vannamei, designated PvGBP2 (FLKLGRKSRYGMLKL), was screened and its antibacterial effect and mechanism of action on the isolated Bacillus spp. explored. The minimal inhibitory concentrations (MIC) of PvGBP2 on Bacillus spp. were 15.6–31.25 μg/mL. Peptide PvGBP2 could inhibit Bacillus subtilis in A. auricular culture bags to maintain growth and yield of A. auricular. Transmission electron microscopy (TEM) revealed that PvGBP2 kills bacteria by perforating the cell wall, destroying membrane integrity and resulting in the leakage of intracellular solutes. In addition, PvGBP2 inhibits biofilm formation by B. subtilis by 90.6 % at 1 × MIC. Thus, peptide PvGBP2 could be potentially applied as an antibacterial agent to control bacterial infection of A. auricular cultivation and the spread of foodborne pathogens. |
| format | Article |
| id | doaj-art-9415be78d45a4b32bdfe6592524d2d1f |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-9415be78d45a4b32bdfe6592524d2d1f2025-02-03T06:56:02ZengTsinghua University PressFood Science and Human Wellness2213-45302022-11-0111616071613Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bagsShen Yang0Zijin Yuan1Jude Juventus Aweya2Shanggui Deng3Wuyin Weng4Yueling Zhang5Guangming Liu6College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Jimei University, Xiamen 361021, ChinaCollege of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Jimei University, Xiamen 361021, ChinaDepartment of Biology, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, ChinaCollege of Food and Pharmacy, Zhejiang Ocean University, Zhoushan 316000, ChinaCollege of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Jimei University, Xiamen 361021, ChinaDepartment of Biology, Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou 515063, China; Corresponding authos.College of Food and Biological Engineering, Xiamen Key Laboratory of Marine Functional Food, Jimei University, Xiamen 361021, China; Corresponding authos.Bacteria contamination in Auricularia auricular culture bags reduces yield and increases the risk of food safety. In this study, 5 species of bacteria, mainly gram-positive bacteria including three species of Bacillus spp., Arthrobacter arilaitensis and Staphylococcus warneri, were isolated and identified from bacteria-contaminated A. auricular culture bags. An in silico predicted antimicrobial peptide from the β-1,3-glucan-binding protein sequence of Penaeus vannamei, designated PvGBP2 (FLKLGRKSRYGMLKL), was screened and its antibacterial effect and mechanism of action on the isolated Bacillus spp. explored. The minimal inhibitory concentrations (MIC) of PvGBP2 on Bacillus spp. were 15.6–31.25 μg/mL. Peptide PvGBP2 could inhibit Bacillus subtilis in A. auricular culture bags to maintain growth and yield of A. auricular. Transmission electron microscopy (TEM) revealed that PvGBP2 kills bacteria by perforating the cell wall, destroying membrane integrity and resulting in the leakage of intracellular solutes. In addition, PvGBP2 inhibits biofilm formation by B. subtilis by 90.6 % at 1 × MIC. Thus, peptide PvGBP2 could be potentially applied as an antibacterial agent to control bacterial infection of A. auricular cultivation and the spread of foodborne pathogens.http://www.sciencedirect.com/science/article/pii/S2213453022001021Auricularia auricularAntimicrobial peptidePenaeus vannameiβ-1,3-Glucan-binding proteinBacillus spp |
| spellingShingle | Shen Yang Zijin Yuan Jude Juventus Aweya Shanggui Deng Wuyin Weng Yueling Zhang Guangming Liu Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags Food Science and Human Wellness Auricularia auricular Antimicrobial peptide Penaeus vannamei β-1,3-Glucan-binding protein Bacillus spp |
| title | Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags |
| title_full | Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags |
| title_fullStr | Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags |
| title_full_unstemmed | Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags |
| title_short | Antibacterial and antibiofilm activity of peptide PvGBP2 against pathogenic bacteria that contaminate Auricularia auricular culture bags |
| title_sort | antibacterial and antibiofilm activity of peptide pvgbp2 against pathogenic bacteria that contaminate auricularia auricular culture bags |
| topic | Auricularia auricular Antimicrobial peptide Penaeus vannamei β-1,3-Glucan-binding protein Bacillus spp |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001021 |
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