Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells
The main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the steroidogenic capacity of Leydig cells in vitro. In four separate experiments, male CFLP mice were exposed to sinusoidal 50-Hz, 100-μT MF. The duration of exposure was 23.5 h/day over a...
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| Format: | Article |
| Language: | English |
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Wiley
2004-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2004.182 |
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| author | Zsolt Forgács Zoltán Somosy Györgyi Kubinyi Hanna Sinay Jozsef Bakos György Thuróczy András Surján Aranka Hudák Ferenc Olajos Péter Lázár |
| author_facet | Zsolt Forgács Zoltán Somosy Györgyi Kubinyi Hanna Sinay Jozsef Bakos György Thuróczy András Surján Aranka Hudák Ferenc Olajos Péter Lázár |
| author_sort | Zsolt Forgács |
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| description | The main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the steroidogenic capacity of Leydig cells in vitro. In four separate experiments, male CFLP mice were exposed to sinusoidal 50-Hz, 100-μT MF. The duration of exposure was 23.5 h/day over a period of 14 days. At the end of the exposure, interstitial (Leydig) cells were isolated from the testicles of the sham-exposed and exposed animals. The cells were cultured for 48 h in the presence or absence of 1, 10, or 100 mIU/ml human chorionic gonadotropin (hCG). The luteinizing hormone (LH) analog hCG was used to check the testosterone (T) response of the sham-exposed controls and to evaluate the possible effect of the whole-body MF exposure on the steroidogenic capacity of Leydig cells in vitro. Testosterone content of the culture media and blood sera was measured by radioimmunoassay (RIA). In the cultures obtained from MF-exposed animals, the hCG-stimulated T response was significanly higher (p < 0.01) compared with the sham-exposed controls, while the basal T production of cells and the level of serum T remained unaltered. No MF exposurerelated histopathological alterations were found in testicles, epididymes, adrenals, prostates, and pituitary glands. The MF exposure did not affect the animal growth rate and the observed hematologic and serum chemical variables. Our results indicate a presumably direct effect of whole-body MF exposure on the hCG-stimulated steroidogenic response of mouse Leydig cells. |
| format | Article |
| id | doaj-art-941379a205764e309dd585ae23107c0b |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2004-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-941379a205764e309dd585ae23107c0b2025-02-03T01:31:16ZengWileyThe Scientific World Journal1537-744X2004-01-014839010.1100/tsw.2004.182Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig CellsZsolt Forgács0Zoltán Somosy1Györgyi Kubinyi2Hanna Sinay3Jozsef Bakos4György Thuróczy5András Surján6Aranka Hudák7Ferenc Olajos8Péter Lázár9National Institute of Chemical Safety, Budapest, Hungary“National Frederic Joliot-Curie” Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary“National Frederic Joliot-Curie” Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary“National Frederic Joliot-Curie” Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary“National Frederic Joliot-Curie” Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary“National Frederic Joliot-Curie” Research Institute for Radiobiology and Radiohygiene, Budapest, HungaryNational Institute of Chemical Safety, Budapest, HungaryClinical Laboratory, National Institute of Occupational Health, Budapest, HungarySecond Department of Gynecology, Semmelweis University, Budapest, HungaryNational Institute of Chemical Safety, Budapest, HungaryThe main goal of this study was to evaluate the possible effect of whole-body magnetic field (MF) exposure on the steroidogenic capacity of Leydig cells in vitro. In four separate experiments, male CFLP mice were exposed to sinusoidal 50-Hz, 100-μT MF. The duration of exposure was 23.5 h/day over a period of 14 days. At the end of the exposure, interstitial (Leydig) cells were isolated from the testicles of the sham-exposed and exposed animals. The cells were cultured for 48 h in the presence or absence of 1, 10, or 100 mIU/ml human chorionic gonadotropin (hCG). The luteinizing hormone (LH) analog hCG was used to check the testosterone (T) response of the sham-exposed controls and to evaluate the possible effect of the whole-body MF exposure on the steroidogenic capacity of Leydig cells in vitro. Testosterone content of the culture media and blood sera was measured by radioimmunoassay (RIA). In the cultures obtained from MF-exposed animals, the hCG-stimulated T response was significanly higher (p < 0.01) compared with the sham-exposed controls, while the basal T production of cells and the level of serum T remained unaltered. No MF exposurerelated histopathological alterations were found in testicles, epididymes, adrenals, prostates, and pituitary glands. The MF exposure did not affect the animal growth rate and the observed hematologic and serum chemical variables. Our results indicate a presumably direct effect of whole-body MF exposure on the hCG-stimulated steroidogenic response of mouse Leydig cells.http://dx.doi.org/10.1100/tsw.2004.182 |
| spellingShingle | Zsolt Forgács Zoltán Somosy Györgyi Kubinyi Hanna Sinay Jozsef Bakos György Thuróczy András Surján Aranka Hudák Ferenc Olajos Péter Lázár Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells The Scientific World Journal |
| title | Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells |
| title_full | Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells |
| title_fullStr | Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells |
| title_full_unstemmed | Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells |
| title_short | Effects of Whole-Body 50-Hz Magnetic Field Exposure on Mouse Leydig Cells |
| title_sort | effects of whole body 50 hz magnetic field exposure on mouse leydig cells |
| url | http://dx.doi.org/10.1100/tsw.2004.182 |
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