Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed
Lipin-1 is a member of the evolutionarily conserved family of proteins and is expressed predominantly in adipose tissue and skeletal muscle. On the one hand, lipin-1 is an enzyme that catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol (DAG) and thus participates in the metabolic...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-03-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/4062 |
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| author | A. V. Igoshin T. M. Mishakova R. B. Aitnazarov A. V. Ilina D. M. Larkin N. S. Yudin |
| author_facet | A. V. Igoshin T. M. Mishakova R. B. Aitnazarov A. V. Ilina D. M. Larkin N. S. Yudin |
| author_sort | A. V. Igoshin |
| collection | DOAJ |
| description | Lipin-1 is a member of the evolutionarily conserved family of proteins and is expressed predominantly in adipose tissue and skeletal muscle. On the one hand, lipin-1 is an enzyme that catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol (DAG) and thus participates in the metabolic pathways of biosynthesis of storage lipids in the cell, membrane phospholipids, and intracellular signaling molecules. On the other hand, lipin-1 is able to be transported from the cytoplasm to the nucleus and is a coactivator of lipid metabolism gene transcription. It was shown, using the analysis of single nucleotide polymorphism (SNP) associations, that the lipin-1 coding gene (LPIN1) is a promising candidate gene for milk production traits in Holstein and Brown Swiss cows. However, it is unclear how much of its effect depends on the breed. The Yaroslavl dairy cattle breed was created in the 18–19 centuries in Russia by breeding northern Great Russian cattle, which were short and poor productive, but well adapted to local climatic conditions and bad food base. It was shown by whole genome genotyping and sequencing that the Yaroslavl breed has unique genetics compared to Russian and other cattle breeds. The aim of the study was to assess the frequency of alleles and genotypes of three SNPs in the LPIN1 gene and to study the association of these SNPs with milk production traits in Yaroslavl cows. Blood samples from 142 cows of the Yaroslavl breed were obtained from two farms in the Yaroslavl region. Genotyping of SNPs was carried out by polymerase chain reaction-restriction fragment length polymorphism method. Associations of SNPs with 305-day milk yield, fat yield, fat percentages, protein yield, and protein percentages were studied from the first to the fourth lactation. Statistical tests were carried out using a mixed linear model, taking into account the relationship between individuals. We identified three SNPs – rs110871255, rs207681322 and rs109039955 with a frequency of a rare allele of 0.042–0.261 in Yaroslavl cows. SNP rs110871255 was associated with fat yield during the third and fourth lactations. SNP rs207681322 was associated with milk yield for the second, third and fourth lactations, as well as protein yield for the third lactation. Thus, we identified significant associations of SNPs rs207681322 and rs110871255 in the LPIN1 gene with a number of milk production traits during several lactations in Yaroslavl cows. |
| format | Article |
| id | doaj-art-93efd8ddb58246219b630633280047c1 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-93efd8ddb58246219b630633280047c12025-02-01T09:58:13ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-03-0128111712510.18699/vjgb-24-141446Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breedA. V. Igoshin0T. M. Mishakova1R. B. Aitnazarov2A. V. Ilina3D. M. Larkin4N. S. Yudin5Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesFederal Williams Research Center for Forage Production and Agroecology, Scientific Research Institute of Livestock Breeding and Forage ProductionRoyal Veterinary College, University of LondonInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesLipin-1 is a member of the evolutionarily conserved family of proteins and is expressed predominantly in adipose tissue and skeletal muscle. On the one hand, lipin-1 is an enzyme that catalyzes the dephosphorylation of phosphatidic acid to diacylglycerol (DAG) and thus participates in the metabolic pathways of biosynthesis of storage lipids in the cell, membrane phospholipids, and intracellular signaling molecules. On the other hand, lipin-1 is able to be transported from the cytoplasm to the nucleus and is a coactivator of lipid metabolism gene transcription. It was shown, using the analysis of single nucleotide polymorphism (SNP) associations, that the lipin-1 coding gene (LPIN1) is a promising candidate gene for milk production traits in Holstein and Brown Swiss cows. However, it is unclear how much of its effect depends on the breed. The Yaroslavl dairy cattle breed was created in the 18–19 centuries in Russia by breeding northern Great Russian cattle, which were short and poor productive, but well adapted to local climatic conditions and bad food base. It was shown by whole genome genotyping and sequencing that the Yaroslavl breed has unique genetics compared to Russian and other cattle breeds. The aim of the study was to assess the frequency of alleles and genotypes of three SNPs in the LPIN1 gene and to study the association of these SNPs with milk production traits in Yaroslavl cows. Blood samples from 142 cows of the Yaroslavl breed were obtained from two farms in the Yaroslavl region. Genotyping of SNPs was carried out by polymerase chain reaction-restriction fragment length polymorphism method. Associations of SNPs with 305-day milk yield, fat yield, fat percentages, protein yield, and protein percentages were studied from the first to the fourth lactation. Statistical tests were carried out using a mixed linear model, taking into account the relationship between individuals. We identified three SNPs – rs110871255, rs207681322 and rs109039955 with a frequency of a rare allele of 0.042–0.261 in Yaroslavl cows. SNP rs110871255 was associated with fat yield during the third and fourth lactations. SNP rs207681322 was associated with milk yield for the second, third and fourth lactations, as well as protein yield for the third lactation. Thus, we identified significant associations of SNPs rs207681322 and rs110871255 in the LPIN1 gene with a number of milk production traits during several lactations in Yaroslavl cows.https://vavilov.elpub.ru/jour/article/view/4062cowyaroslavl breedmilk yieldfat percentageprotein percentagefat yieldprotein yield<i>lpin1</i> genesingle nucleotide polymorphismassociation |
| spellingShingle | A. V. Igoshin T. M. Mishakova R. B. Aitnazarov A. V. Ilina D. M. Larkin N. S. Yudin Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed Вавиловский журнал генетики и селекции cow yaroslavl breed milk yield fat percentage protein percentage fat yield protein yield <i>lpin1</i> gene single nucleotide polymorphism association |
| title | Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed |
| title_full | Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed |
| title_fullStr | Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed |
| title_full_unstemmed | Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed |
| title_short | Association of three single nucleotide polymorphisms in the <i>LPIN1</i> gene with milk production traits in cows of the Yaroslavl breed |
| title_sort | association of three single nucleotide polymorphisms in the i lpin1 i gene with milk production traits in cows of the yaroslavl breed |
| topic | cow yaroslavl breed milk yield fat percentage protein percentage fat yield protein yield <i>lpin1</i> gene single nucleotide polymorphism association |
| url | https://vavilov.elpub.ru/jour/article/view/4062 |
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