Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers
This research introduced a strategy to fabricate sub-millimeter-diameter artificial liver tissue by extruding a combination of a liver decellularized extracellular matrix (dECM), alginate, endothelial cells, and hepatocytes. Vascularization remains a critical challenge in liver tissue engineering, a...
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| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/16/1/35 |
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| author | Chanh-Trung Nguyen Van Phu Le Thi Huong Le Jeong Sook Kim Sung Hoon Back Kyo-in Koo |
| author_facet | Chanh-Trung Nguyen Van Phu Le Thi Huong Le Jeong Sook Kim Sung Hoon Back Kyo-in Koo |
| author_sort | Chanh-Trung Nguyen |
| collection | DOAJ |
| description | This research introduced a strategy to fabricate sub-millimeter-diameter artificial liver tissue by extruding a combination of a liver decellularized extracellular matrix (dECM), alginate, endothelial cells, and hepatocytes. Vascularization remains a critical challenge in liver tissue engineering, as replicating the liver’s intricate vascular network is essential for sustaining cellular function and viability. Seven scaffold groups were evaluated, incorporating different cell compositions, scaffold materials, and structural configurations. The hepatocyte and endothelial cell scaffold treated with alginate lyase demonstrated the highest diffusion rate, along with enhanced albumin secretion (2.8 µg/mL) and urea synthesis (220 µg/mL) during the same period by day 10. A dense and interconnected endothelial cell network was observed as early as day 4 in the lyased coculture group. Furthermore, three-week implantation studies in rats showed a stable integration to the host with no adverse effects. This approach offers significant potential for advancing functional liver tissue replacements, combining accelerated diffusion, enhanced albumin secretion, improved urea synthesis, dense vascular network formation, and stable implantation outcomes. |
| format | Article |
| id | doaj-art-93e3a4b8b37a487c816dc67f780b4b31 |
| institution | Kabale University |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-93e3a4b8b37a487c816dc67f780b4b312025-01-24T13:36:12ZengMDPI AGJournal of Functional Biomaterials2079-49832025-01-011613510.3390/jfb16010035Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial LiversChanh-Trung Nguyen0Van Phu Le1Thi Huong Le2Jeong Sook Kim3Sung Hoon Back4Kyo-in Koo5Department of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan 44610, Republic of KoreaDepartment of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan 44610, Republic of KoreaDepartment of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan 44610, Republic of KoreaDepartment of Obstetrics and Gynecology, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan 44033, Republic of KoreaSchool of Biological Sciences, University of Ulsan, Ulsan 44610, Republic of KoreaDepartment of Electrical, Electronic and Computer Engineering, University of Ulsan, Ulsan 44610, Republic of KoreaThis research introduced a strategy to fabricate sub-millimeter-diameter artificial liver tissue by extruding a combination of a liver decellularized extracellular matrix (dECM), alginate, endothelial cells, and hepatocytes. Vascularization remains a critical challenge in liver tissue engineering, as replicating the liver’s intricate vascular network is essential for sustaining cellular function and viability. Seven scaffold groups were evaluated, incorporating different cell compositions, scaffold materials, and structural configurations. The hepatocyte and endothelial cell scaffold treated with alginate lyase demonstrated the highest diffusion rate, along with enhanced albumin secretion (2.8 µg/mL) and urea synthesis (220 µg/mL) during the same period by day 10. A dense and interconnected endothelial cell network was observed as early as day 4 in the lyased coculture group. Furthermore, three-week implantation studies in rats showed a stable integration to the host with no adverse effects. This approach offers significant potential for advancing functional liver tissue replacements, combining accelerated diffusion, enhanced albumin secretion, improved urea synthesis, dense vascular network formation, and stable implantation outcomes.https://www.mdpi.com/2079-4983/16/1/35decellularized extracellular matriximplantationliver tissuealginatealginate lyase |
| spellingShingle | Chanh-Trung Nguyen Van Phu Le Thi Huong Le Jeong Sook Kim Sung Hoon Back Kyo-in Koo Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers Journal of Functional Biomaterials decellularized extracellular matrix implantation liver tissue alginate alginate lyase |
| title | Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers |
| title_full | Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers |
| title_fullStr | Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers |
| title_full_unstemmed | Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers |
| title_short | Sacrificing Alginate in Decellularized Extracellular Matrix Scaffolds for Implantable Artificial Livers |
| title_sort | sacrificing alginate in decellularized extracellular matrix scaffolds for implantable artificial livers |
| topic | decellularized extracellular matrix implantation liver tissue alginate alginate lyase |
| url | https://www.mdpi.com/2079-4983/16/1/35 |
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