Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency
Using livestock manure as organic fertilizer on farmlands has resulted in increased crop yields in many areas; however, the impact of long-term manure application on N cycling microorganisms and their effect on nitrogen utilization efficiency (NUE) associated with increased maize yield remains uncle...
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Elsevier
2025-08-01
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| Series: | Geoderma |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0016706125002368 |
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| author | Yansheng Li Muqing Liu Zhenhua Yu Changkai Liu Xiaojing Hu Junjie Liu Jian Jin Yuan Chen Xingyi Zhang Guanghua Wang Xiaobing Liu |
| author_facet | Yansheng Li Muqing Liu Zhenhua Yu Changkai Liu Xiaojing Hu Junjie Liu Jian Jin Yuan Chen Xingyi Zhang Guanghua Wang Xiaobing Liu |
| author_sort | Yansheng Li |
| collection | DOAJ |
| description | Using livestock manure as organic fertilizer on farmlands has resulted in increased crop yields in many areas; however, the impact of long-term manure application on N cycling microorganisms and their effect on nitrogen utilization efficiency (NUE) associated with increased maize yield remains unclear. In this study, a long-term field trial was performed to compare four fertilization regimes: no fertilizer (CK), conventional synthetic fertilizer (CF), CF with 15 Mg ha−1 (FM1) and 30 Mg ha−1 (FM2) of dry-weight cattle manure, applied annually. After 10 and 12 years, FM1 and FM2 significantly boosted maize yield, especially after cycles of soybean–maize rotation, and improved NUE by 19–33 % and 17–53 %, respectively, compared with CF, with a positive correlation with increases in yield. Principal coordinate analysis showed that different fertilizer regimes were partitioned into four groups of N-related microbial communities. Proteobacteria, Actinobacteria, and Acidobacteria were the main phyla involved in N cycling. Among the genes involved in N cycling, gdhA, narB, nasD, norB, napA, nirB, nifA and hao demonstrated significant correlations with both plant N uptake and NUE. The insignificant differences between FM1 and FM2 in the relative abundance of most N genes in 2021 and 2023 helped to explain the similar crop outcomes between these two treatments. Compared with FM1, FM2 had a higher relative abundance of narG and a smaller size of constructed metagenome-assembled genomes (MAGs). |
| format | Article |
| id | doaj-art-93e0636a1b8a4ac4a913e878b52c6104 |
| institution | Kabale University |
| issn | 1872-6259 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Geoderma |
| spelling | doaj-art-93e0636a1b8a4ac4a913e878b52c61042025-08-20T04:00:34ZengElsevierGeoderma1872-62592025-08-0146011739810.1016/j.geoderma.2025.117398Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiencyYansheng Li0Muqing Liu1Zhenhua Yu2Changkai Liu3Xiaojing Hu4Junjie Liu5Jian Jin6Yuan Chen7Xingyi Zhang8Guanghua Wang9Xiaobing Liu10State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaJilin Agricultural University, 2888 Xincheng Street, Changchun 130118, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, China; Corresponding author.State Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, China; La Trobe Institute for Sustainable Agriculture and Food, Department of Ecological, Plant and Animal Sciences, La Trobe University, Bundoora, Vic 3086, AustraliaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaState Key Laboratory of Black Soils Conservation and Utilization, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, 138 Haping Road, Harbin 150081, ChinaUsing livestock manure as organic fertilizer on farmlands has resulted in increased crop yields in many areas; however, the impact of long-term manure application on N cycling microorganisms and their effect on nitrogen utilization efficiency (NUE) associated with increased maize yield remains unclear. In this study, a long-term field trial was performed to compare four fertilization regimes: no fertilizer (CK), conventional synthetic fertilizer (CF), CF with 15 Mg ha−1 (FM1) and 30 Mg ha−1 (FM2) of dry-weight cattle manure, applied annually. After 10 and 12 years, FM1 and FM2 significantly boosted maize yield, especially after cycles of soybean–maize rotation, and improved NUE by 19–33 % and 17–53 %, respectively, compared with CF, with a positive correlation with increases in yield. Principal coordinate analysis showed that different fertilizer regimes were partitioned into four groups of N-related microbial communities. Proteobacteria, Actinobacteria, and Acidobacteria were the main phyla involved in N cycling. Among the genes involved in N cycling, gdhA, narB, nasD, norB, napA, nirB, nifA and hao demonstrated significant correlations with both plant N uptake and NUE. The insignificant differences between FM1 and FM2 in the relative abundance of most N genes in 2021 and 2023 helped to explain the similar crop outcomes between these two treatments. Compared with FM1, FM2 had a higher relative abundance of narG and a smaller size of constructed metagenome-assembled genomes (MAGs).http://www.sciencedirect.com/science/article/pii/S0016706125002368NUESoil fertilitySoil microbial communityTillage practiceCrop yield |
| spellingShingle | Yansheng Li Muqing Liu Zhenhua Yu Changkai Liu Xiaojing Hu Junjie Liu Jian Jin Yuan Chen Xingyi Zhang Guanghua Wang Xiaobing Liu Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency Geoderma NUE Soil fertility Soil microbial community Tillage practice Crop yield |
| title | Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency |
| title_full | Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency |
| title_fullStr | Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency |
| title_full_unstemmed | Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency |
| title_short | Long-term application of cattle manure alters functional N cycling genes and improves maize yield and nitrogen use efficiency |
| title_sort | long term application of cattle manure alters functional n cycling genes and improves maize yield and nitrogen use efficiency |
| topic | NUE Soil fertility Soil microbial community Tillage practice Crop yield |
| url | http://www.sciencedirect.com/science/article/pii/S0016706125002368 |
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