Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney
Polyvinyl chloride microplastics (PVC-MPs) and Cadmium (Cd) are widely occurring water pollutants that interact with each other to exert toxic effects. As a waterfowl, Muscovy duck is more susceptible to PVC-MPs and Cd than land poultry. In this study, Muscovy duck was used as a research model, and...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912401068X |
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| author | Hui Zou Jie Song Xianzu Luo Waseem Ali Sifan Li Ling Xiong Yan Chen Yan Yuan Yonggang Ma Xishuai Tong Zongping Liu |
| author_facet | Hui Zou Jie Song Xianzu Luo Waseem Ali Sifan Li Ling Xiong Yan Chen Yan Yuan Yonggang Ma Xishuai Tong Zongping Liu |
| author_sort | Hui Zou |
| collection | DOAJ |
| description | Polyvinyl chloride microplastics (PVC-MPs) and Cadmium (Cd) are widely occurring water pollutants that interact with each other to exert toxic effects. As a waterfowl, Muscovy duck is more susceptible to PVC-MPs and Cd than land poultry. In this study, Muscovy duck was used as a research model, and 10 mg/L PVC-MPs and 50 mg/kg Cd were used alone and in combine to explore the effect on the kidney of Muscovy duck. We found that treatment of Cd or PVC-MPs alone changed the kidney weight, increased creatinine and urea nitrogen content, and disrupted oxidative balance and macro/trace element metabolism, while the combination of PVC-MPs+Cd reduced the accumulation of Cd in the kidney. In addition, treatment of Cd and PVC-MPs alone caused mitochondrial damage, increase or decrease of mitochondria-associated proteins (Fis1, Drp1, PGC-1α, Nrf1), and Nrf2 signaling pathway plays a key role in detoxification and alleviation of oxidative stress, and we found that PVC-MPs+Cd treatment recovered related proteins (Nrf2, Keap-1, HO-1, NQO1, AC-SOD2, SOD2) compared with the Cd and PVC-MPs alone treatment. Finally, we detected changes in apoptosis-related proteins and genes (Caspase-3, Caspase-9, Bax, Bcl-2, Cytc) and TUNEL staining, and after PVC-MPs+Cd treatment, apoptosis-related proteins/genes recovered and the apoptosis rate decreased compared with the Cd and PVC-MPs alone treatment. These results indicate that renal function is impaired, oxidative stress and trace element metabolism disorder, nuclear factor-E2 related factor 2 (Nrf2) is activated into the nucleus to induce the expression of related antioxidant proteins (such as HO-1, NQO1). These injuries can induce mitochondrial damage and eventually lead to renal cell apoptosis. To sum up, these evidence show that Cd or PVC-MPs can induce kidney oxidative damage, trace element metabolism disorder, mitochondrial damage and apoptosis. |
| format | Article |
| id | doaj-art-93cb20381a2f4a8da9feeaedcde7b81a |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-93cb20381a2f4a8da9feeaedcde7b81a2025-01-22T05:40:14ZengElsevierPoultry Science0032-57912025-01-011041104490Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidneyHui Zou0Jie Song1Xianzu Luo2Waseem Ali3Sifan Li4Ling Xiong5Yan Chen6Yan Yuan7Yonggang Ma8Xishuai Tong9Zongping Liu10College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR China; Correspondence author at: College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China.College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaAgri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, Jiangsu 225009, PR ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, Jiangsu 225009, PR ChinaPolyvinyl chloride microplastics (PVC-MPs) and Cadmium (Cd) are widely occurring water pollutants that interact with each other to exert toxic effects. As a waterfowl, Muscovy duck is more susceptible to PVC-MPs and Cd than land poultry. In this study, Muscovy duck was used as a research model, and 10 mg/L PVC-MPs and 50 mg/kg Cd were used alone and in combine to explore the effect on the kidney of Muscovy duck. We found that treatment of Cd or PVC-MPs alone changed the kidney weight, increased creatinine and urea nitrogen content, and disrupted oxidative balance and macro/trace element metabolism, while the combination of PVC-MPs+Cd reduced the accumulation of Cd in the kidney. In addition, treatment of Cd and PVC-MPs alone caused mitochondrial damage, increase or decrease of mitochondria-associated proteins (Fis1, Drp1, PGC-1α, Nrf1), and Nrf2 signaling pathway plays a key role in detoxification and alleviation of oxidative stress, and we found that PVC-MPs+Cd treatment recovered related proteins (Nrf2, Keap-1, HO-1, NQO1, AC-SOD2, SOD2) compared with the Cd and PVC-MPs alone treatment. Finally, we detected changes in apoptosis-related proteins and genes (Caspase-3, Caspase-9, Bax, Bcl-2, Cytc) and TUNEL staining, and after PVC-MPs+Cd treatment, apoptosis-related proteins/genes recovered and the apoptosis rate decreased compared with the Cd and PVC-MPs alone treatment. These results indicate that renal function is impaired, oxidative stress and trace element metabolism disorder, nuclear factor-E2 related factor 2 (Nrf2) is activated into the nucleus to induce the expression of related antioxidant proteins (such as HO-1, NQO1). These injuries can induce mitochondrial damage and eventually lead to renal cell apoptosis. To sum up, these evidence show that Cd or PVC-MPs can induce kidney oxidative damage, trace element metabolism disorder, mitochondrial damage and apoptosis.http://www.sciencedirect.com/science/article/pii/S003257912401068XApoptosisCadmiumDuck kidneyOxidative stressPVC-MPs |
| spellingShingle | Hui Zou Jie Song Xianzu Luo Waseem Ali Sifan Li Ling Xiong Yan Chen Yan Yuan Yonggang Ma Xishuai Tong Zongping Liu Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney Poultry Science Apoptosis Cadmium Duck kidney Oxidative stress PVC-MPs |
| title | Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney |
| title_full | Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney |
| title_fullStr | Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney |
| title_full_unstemmed | Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney |
| title_short | Cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney |
| title_sort | cadmium and polyvinyl chloride microplastics induce mitochondrial damage and apoptosis under oxidative stress in duck kidney |
| topic | Apoptosis Cadmium Duck kidney Oxidative stress PVC-MPs |
| url | http://www.sciencedirect.com/science/article/pii/S003257912401068X |
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