Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research
Cellular heterogeneity is a fundamental characteristic of many cancers. A lack of cellular homogeneity contributes to difficulty in designing targeted oncological therapies. Therefore, the development of novel methods to determine and characterize oncologic cellular heterogeneity is a critical next...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2016/9369240 |
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| author | Baixin Ye Qingping Gao Zhi Zeng Creed M. Stary Zhihong Jian Xiaoxing Xiong Lijuan Gu |
| author_facet | Baixin Ye Qingping Gao Zhi Zeng Creed M. Stary Zhihong Jian Xiaoxing Xiong Lijuan Gu |
| author_sort | Baixin Ye |
| collection | DOAJ |
| description | Cellular heterogeneity is a fundamental characteristic of many cancers. A lack of cellular homogeneity contributes to difficulty in designing targeted oncological therapies. Therefore, the development of novel methods to determine and characterize oncologic cellular heterogeneity is a critical next step in the development of novel cancer therapies. Single-cell sequencing (SCS) technology has been recently employed for analyzing the genetic polymorphisms of individual cells at the genome-wide level. SCS requires (1) precise isolation of the single cell of interest; (2) isolation and amplification of genetic material; and (3) descriptive analysis of genomic, transcriptomic, and epigenomic data. In addition to targeted analysis of single cells isolated from tumor biopsies, SCS technology may be applied to circulating tumor cells, which may aid in predicting tumor progression and metastasis. In this paper, we provide an overview of SCS technology and review the current literature on the potential application of SCS to clinical oncology and research. |
| format | Article |
| id | doaj-art-93c8e4cfbb9f4b008dff46d3279ce120 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-93c8e4cfbb9f4b008dff46d3279ce1202025-02-03T05:52:39ZengWileyAnalytical Cellular Pathology2210-71772210-71852016-01-01201610.1155/2016/93692409369240Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and ResearchBaixin Ye0Qingping Gao1Zhi Zeng2Creed M. Stary3Zhihong Jian4Xiaoxing Xiong5Lijuan Gu6Department of Hematology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Hematology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Pathology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Anesthesiology, Perioperative and Pain Medicine, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaDepartment of Neurosurgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaCentral Laboratory, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, ChinaCellular heterogeneity is a fundamental characteristic of many cancers. A lack of cellular homogeneity contributes to difficulty in designing targeted oncological therapies. Therefore, the development of novel methods to determine and characterize oncologic cellular heterogeneity is a critical next step in the development of novel cancer therapies. Single-cell sequencing (SCS) technology has been recently employed for analyzing the genetic polymorphisms of individual cells at the genome-wide level. SCS requires (1) precise isolation of the single cell of interest; (2) isolation and amplification of genetic material; and (3) descriptive analysis of genomic, transcriptomic, and epigenomic data. In addition to targeted analysis of single cells isolated from tumor biopsies, SCS technology may be applied to circulating tumor cells, which may aid in predicting tumor progression and metastasis. In this paper, we provide an overview of SCS technology and review the current literature on the potential application of SCS to clinical oncology and research.http://dx.doi.org/10.1155/2016/9369240 |
| spellingShingle | Baixin Ye Qingping Gao Zhi Zeng Creed M. Stary Zhihong Jian Xiaoxing Xiong Lijuan Gu Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research Analytical Cellular Pathology |
| title | Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research |
| title_full | Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research |
| title_fullStr | Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research |
| title_full_unstemmed | Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research |
| title_short | Single-Cell Sequencing Technology in Oncology: Applications for Clinical Therapies and Research |
| title_sort | single cell sequencing technology in oncology applications for clinical therapies and research |
| url | http://dx.doi.org/10.1155/2016/9369240 |
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