Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms
Pseudomonas aeruginosa is a hard-to-treat human pathogen for which new antimicrobial agents are urgently needed. P. aeruginosa is known for forming biofilms, a complex aggregate of bacteria embedded in a self-generated protective matrix that enhance its resistance to antibiotics and the immune syste...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-02-01
|
| Series: | Photodiagnosis and Photodynamic Therapy |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1572100024004927 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575534981185536 |
|---|---|
| author | Mona Mahmoud Peter Richter Philipp Arnold Michael Lebert Andreas Burkovski |
| author_facet | Mona Mahmoud Peter Richter Philipp Arnold Michael Lebert Andreas Burkovski |
| author_sort | Mona Mahmoud |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a hard-to-treat human pathogen for which new antimicrobial agents are urgently needed. P. aeruginosa is known for forming biofilms, a complex aggregate of bacteria embedded in a self-generated protective matrix that enhance its resistance to antibiotics and the immune system. Within the biofilm, persister cells, sub-populations of slow-growing or growth-arrested cells, are associated with recalcitrance of infections and antibiotic treatment failure. Here, we investigate the influence of the anionic photosensitiser chlorophyllin (CHL)11 Abbreviations: CHL: Chlorophyllin; BP: Butylparaben; SHX: Serine hydroxamate; TEM: Transmission electron microscope; AMR: Antimicrobial resistance; aPDI: Antimicrobial photodynamic inactivation; LED: Light-emitting diodes exposed to red light alone and in combination with an activator of the mechanosensitive channels butylparaben (BP) on P. aeruginosa growing cells, persister cells, and biofilms. Antimicrobial susceptibility tests were performed using the broth microdilution checkerboard method. Serine hydroxamate (SHX) was used for the induction of persister cells. Under illumination, a combination of CHL (250 µg/ml) and BP (97.12 µg/ml) reduced the number of growing cells and persister cells by 2.2±0.46 log10 and 1.7±0.15 log10, respectively after 30 min of exposure at 79 J/cm2. A higher concentration of BP (194.23 µg/ml) or longer exposure time (60 min at 158 J/cm2) effectively eliminated approximately ≥99.99 % of growing and persister cells. Visual evidence from confocal and TEM images illustrates the influence of CHL and red light, which intensifies when combined with BP. Nevertheless, the addition of BP did not enhance the efficacy of CHL against biofilms; CHL (500 µg/ml) reduced biofilm viability by 2.6 log10 at 791 J/cm2. No toxicity has been observed in darkness. This study highlights the potential antimicrobial effect of CHL against P. aeruginosa. |
| format | Article |
| id | doaj-art-9383a48466bf4c53b32d49ddf0b31fb3 |
| institution | Kabale University |
| issn | 1572-1000 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photodiagnosis and Photodynamic Therapy |
| spelling | doaj-art-9383a48466bf4c53b32d49ddf0b31fb32025-02-01T04:11:46ZengElsevierPhotodiagnosis and Photodynamic Therapy1572-10002025-02-0151104456Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilmsMona Mahmoud0Peter Richter1Philipp Arnold2Michael Lebert3Andreas Burkovski4Department of Biology, Microbiology Division, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, 91058, Germany; Dairy Department, National Research Centre, Dokki, Giza, 12622, EgyptDepartment of Biology, Cell Biology Division, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, 91058, GermanyInstitute of Functional and Clinical Anatomy, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, 91054, GermanyDepartment of Biology, Cell Biology Division, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, 91058, GermanyDepartment of Biology, Microbiology Division, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, 91058, Germany; Corresponding author.Pseudomonas aeruginosa is a hard-to-treat human pathogen for which new antimicrobial agents are urgently needed. P. aeruginosa is known for forming biofilms, a complex aggregate of bacteria embedded in a self-generated protective matrix that enhance its resistance to antibiotics and the immune system. Within the biofilm, persister cells, sub-populations of slow-growing or growth-arrested cells, are associated with recalcitrance of infections and antibiotic treatment failure. Here, we investigate the influence of the anionic photosensitiser chlorophyllin (CHL)11 Abbreviations: CHL: Chlorophyllin; BP: Butylparaben; SHX: Serine hydroxamate; TEM: Transmission electron microscope; AMR: Antimicrobial resistance; aPDI: Antimicrobial photodynamic inactivation; LED: Light-emitting diodes exposed to red light alone and in combination with an activator of the mechanosensitive channels butylparaben (BP) on P. aeruginosa growing cells, persister cells, and biofilms. Antimicrobial susceptibility tests were performed using the broth microdilution checkerboard method. Serine hydroxamate (SHX) was used for the induction of persister cells. Under illumination, a combination of CHL (250 µg/ml) and BP (97.12 µg/ml) reduced the number of growing cells and persister cells by 2.2±0.46 log10 and 1.7±0.15 log10, respectively after 30 min of exposure at 79 J/cm2. A higher concentration of BP (194.23 µg/ml) or longer exposure time (60 min at 158 J/cm2) effectively eliminated approximately ≥99.99 % of growing and persister cells. Visual evidence from confocal and TEM images illustrates the influence of CHL and red light, which intensifies when combined with BP. Nevertheless, the addition of BP did not enhance the efficacy of CHL against biofilms; CHL (500 µg/ml) reduced biofilm viability by 2.6 log10 at 791 J/cm2. No toxicity has been observed in darkness. This study highlights the potential antimicrobial effect of CHL against P. aeruginosa.http://www.sciencedirect.com/science/article/pii/S1572100024004927Antimicrobial resistanceChlorophyllinButylparabenNosocomial infection |
| spellingShingle | Mona Mahmoud Peter Richter Philipp Arnold Michael Lebert Andreas Burkovski Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms Photodiagnosis and Photodynamic Therapy Antimicrobial resistance Chlorophyllin Butylparaben Nosocomial infection |
| title | Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms |
| title_full | Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms |
| title_fullStr | Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms |
| title_full_unstemmed | Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms |
| title_short | Antimicrobial photodynamic inactivation of Pseudomonas aeruginosa persister cells and biofilms |
| title_sort | antimicrobial photodynamic inactivation of pseudomonas aeruginosa persister cells and biofilms |
| topic | Antimicrobial resistance Chlorophyllin Butylparaben Nosocomial infection |
| url | http://www.sciencedirect.com/science/article/pii/S1572100024004927 |
| work_keys_str_mv | AT monamahmoud antimicrobialphotodynamicinactivationofpseudomonasaeruginosapersistercellsandbiofilms AT peterrichter antimicrobialphotodynamicinactivationofpseudomonasaeruginosapersistercellsandbiofilms AT philipparnold antimicrobialphotodynamicinactivationofpseudomonasaeruginosapersistercellsandbiofilms AT michaellebert antimicrobialphotodynamicinactivationofpseudomonasaeruginosapersistercellsandbiofilms AT andreasburkovski antimicrobialphotodynamicinactivationofpseudomonasaeruginosapersistercellsandbiofilms |