Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens
Introduction: Antimicrobial substances (AMS) produced by bacteria may reduce or prevent the growth of pathogenic and spoilage microorganisms in food. In this study, 16 isolates of Acinetobacter baumannii/calcoaceticus (ABC) complex, previously obtained from reconstituted infant milk formula (IMF) s...
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The Journal of Infection in Developing Countries
2018-05-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9894 |
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| author | Cinara Souza da Conceição Barbara Victor Souza Jessica Manya Bittencourt Dias Vieira Janaína dos Santos Nascimento |
| author_facet | Cinara Souza da Conceição Barbara Victor Souza Jessica Manya Bittencourt Dias Vieira Janaína dos Santos Nascimento |
| author_sort | Cinara Souza da Conceição |
| collection | DOAJ |
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Introduction: Antimicrobial substances (AMS) produced by bacteria may reduce or prevent the growth of pathogenic and spoilage microorganisms in food. In this study, 16 isolates of Acinetobacter baumannii/calcoaceticus (ABC) complex, previously obtained from reconstituted infant milk formula (IMF) samples and the preparation and distribution utensils from the nursery of a public hospital, were used to screen for AMS production.
Methodology: Antimicrobial substance production and spectrum of activity assays were performed by agar-spot assay. Optimization of growth conditions for AMS production was also evaluated.
Results: Three (17.6%) isolates, namely JE3, JE4, and JE6, produced AMS against the principal indicator strain Salmonella enterica subsp. enterica serotype Typhi ATCC 19214. JE6 was also able to inhibit strains of Klebsiella pneumoniae, Proteus vulgaris, and Bacillus cereus, a Gram-positive bacteria. Remarkably, JE6 was able to inhibit all the tested resistant and multidrug-resistant (MDR) strains of the ABC complex and Shigella dysenteriae associated with IMF and utensils, indicating a potentially valuable application. AMS produced by JE6 does not appear to be affected by proteolytic enzymes and the producer strain showed specific immunity to its own AMS.
Conclusion: This study highlights AMS produced by Acinetobacter with applications against MDR spoilage and foodborne pathogens - some of them, infectious disease causing agents - which, to our knowledge, has not been previously described.
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| format | Article |
| id | doaj-art-934f642f5b794c64a3473df40c25bc92 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-934f642f5b794c64a3473df40c25bc922025-08-20T02:14:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-05-01120510.3855/jidc.9894Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogensCinara Souza da Conceição0Barbara Victor Souza1Jessica Manya Bittencourt Dias Vieira2Janaína dos Santos Nascimento3Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Rio de Janeiro, BrazilInstituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Rio de Janeiro, BrazilCentro Universitário Estadual da Zona Oeste (UEZO), Rio de Janeiro, BrazilInstituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro (IFRJ), Rio de Janeiro, Brazil Introduction: Antimicrobial substances (AMS) produced by bacteria may reduce or prevent the growth of pathogenic and spoilage microorganisms in food. In this study, 16 isolates of Acinetobacter baumannii/calcoaceticus (ABC) complex, previously obtained from reconstituted infant milk formula (IMF) samples and the preparation and distribution utensils from the nursery of a public hospital, were used to screen for AMS production. Methodology: Antimicrobial substance production and spectrum of activity assays were performed by agar-spot assay. Optimization of growth conditions for AMS production was also evaluated. Results: Three (17.6%) isolates, namely JE3, JE4, and JE6, produced AMS against the principal indicator strain Salmonella enterica subsp. enterica serotype Typhi ATCC 19214. JE6 was also able to inhibit strains of Klebsiella pneumoniae, Proteus vulgaris, and Bacillus cereus, a Gram-positive bacteria. Remarkably, JE6 was able to inhibit all the tested resistant and multidrug-resistant (MDR) strains of the ABC complex and Shigella dysenteriae associated with IMF and utensils, indicating a potentially valuable application. AMS produced by JE6 does not appear to be affected by proteolytic enzymes and the producer strain showed specific immunity to its own AMS. Conclusion: This study highlights AMS produced by Acinetobacter with applications against MDR spoilage and foodborne pathogens - some of them, infectious disease causing agents - which, to our knowledge, has not been previously described. https://jidc.org/index.php/journal/article/view/9894Acinetobacter baumannii/calcoaceticusantimicrobial substancefoodborne pathogensbacteriocin |
| spellingShingle | Cinara Souza da Conceição Barbara Victor Souza Jessica Manya Bittencourt Dias Vieira Janaína dos Santos Nascimento Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens Journal of Infection in Developing Countries Acinetobacter baumannii/calcoaceticus antimicrobial substance foodborne pathogens bacteriocin |
| title | Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens |
| title_full | Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens |
| title_fullStr | Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens |
| title_full_unstemmed | Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens |
| title_short | Pathogen killing pathogen: antimicrobial substance from Acinetobacter active against foodborne pathogens |
| title_sort | pathogen killing pathogen antimicrobial substance from acinetobacter active against foodborne pathogens |
| topic | Acinetobacter baumannii/calcoaceticus antimicrobial substance foodborne pathogens bacteriocin |
| url | https://jidc.org/index.php/journal/article/view/9894 |
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