Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells
Phosphodiesterase 4D (PDE4D) is a member of the large superfamily of phosphodiesterases. PDE4D polymorphisms have been found to associate with ischemic stroke. Proliferation and migration of vascular smooth muscle cells (VSMCs) play a critical role in the pathogenesis of atherosclerosis. In this stu...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Stroke Research and Treatment |
| Online Access: | http://dx.doi.org/10.4061/2011/534257 |
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| author | Lin Liu Xiaowei Xu Jiejie Li Xia Li Wenli Sheng |
| author_facet | Lin Liu Xiaowei Xu Jiejie Li Xia Li Wenli Sheng |
| author_sort | Lin Liu |
| collection | DOAJ |
| description | Phosphodiesterase 4D (PDE4D) is a member of the large superfamily of phosphodiesterases. PDE4D polymorphisms have been found to associate with ischemic stroke. Proliferation and migration of vascular smooth muscle cells (VSMCs) play a critical role in the pathogenesis of atherosclerosis. In this study, infection of VSMCs with lentivrius particles carrying shRNA direct against PDE4D significantly inhibited platelet-derived growth factor-induced VSMC proliferation and migration, and the inhibitory effects were not associated with global intracellular cAMP level. Our results implicate that PDE4D has an important role in VSMC proliferation and migration which may explain its genetic susceptibility to ischemic stroke. |
| format | Article |
| id | doaj-art-931effc3bd954214a1d540dc09d83f0d |
| institution | Kabale University |
| issn | 2042-0056 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stroke Research and Treatment |
| spelling | doaj-art-931effc3bd954214a1d540dc09d83f0d2025-02-03T01:32:22ZengWileyStroke Research and Treatment2042-00562011-01-01201110.4061/2011/534257534257Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle CellsLin Liu0Xiaowei Xu1Jiejie Li2Xia Li3Wenli Sheng4Department of Neurology, Sun Yat-Sen University Tungwah Hospital, Dongguan, ChinaStroke Center Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, ChinaStroke Center Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, ChinaStroke Center Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, ChinaStroke Center Department of Neurology, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, ChinaPhosphodiesterase 4D (PDE4D) is a member of the large superfamily of phosphodiesterases. PDE4D polymorphisms have been found to associate with ischemic stroke. Proliferation and migration of vascular smooth muscle cells (VSMCs) play a critical role in the pathogenesis of atherosclerosis. In this study, infection of VSMCs with lentivrius particles carrying shRNA direct against PDE4D significantly inhibited platelet-derived growth factor-induced VSMC proliferation and migration, and the inhibitory effects were not associated with global intracellular cAMP level. Our results implicate that PDE4D has an important role in VSMC proliferation and migration which may explain its genetic susceptibility to ischemic stroke.http://dx.doi.org/10.4061/2011/534257 |
| spellingShingle | Lin Liu Xiaowei Xu Jiejie Li Xia Li Wenli Sheng Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells Stroke Research and Treatment |
| title | Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells |
| title_full | Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells |
| title_fullStr | Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells |
| title_full_unstemmed | Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells |
| title_short | Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells |
| title_sort | lentiviral mediated shrna silencing of pde4d gene inhibits platelet derived growth factor induced proliferation and migration of rat aortic smooth muscle cells |
| url | http://dx.doi.org/10.4061/2011/534257 |
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