Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry
High oleic-acid peanuts are known for their pre-longed shelf-life and health benefit due to high content of oleic fatty acid. However, the allergenicity and allergenic protein profiles in Chinese high-oleic peanuts have yet to be studied. For this purpose, an Orbitrap Fusion mass spectrometry (MS)-b...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2023-05-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002130 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832544352677658624 |
|---|---|
| author | Xinke Yang Haochen Bai Lijun Yin Jin Wang Wentong Xue Xin Jia |
| author_facet | Xinke Yang Haochen Bai Lijun Yin Jin Wang Wentong Xue Xin Jia |
| author_sort | Xinke Yang |
| collection | DOAJ |
| description | High oleic-acid peanuts are known for their pre-longed shelf-life and health benefit due to high content of oleic fatty acid. However, the allergenicity and allergenic protein profiles in Chinese high-oleic peanuts have yet to be studied. For this purpose, an Orbitrap Fusion mass spectrometry (MS)-based method that is feasible for identification of putative allergenic protein as well as semi-quantitation of five major allergen protein in three different Chinese high-oleic peanut cultivars (JH 13, JH 16 and JH 18) have been reported. Results show that three Chinese high-oleic acid peanut cultivars selected all contained highly allergenic proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6. The allergenic protein profiles of Chinese high-oleic acid peanut cultivars were very similar to that of conventional peanuts, but the allergenic protein subunits varied greatly among high-oleic peanuts. Additionally, a comprehensive peptide-filtering pipeline had been developed for identification of potential peptide markers in peanut allergen proteins. Through the peptide-filtering pipeline, three novel peptide markers, IVQIEAKPNTLVLPK, SSNPDIYNPQAGSLR and AQSENYEYLAFK surrogate to Ara h 1, Ara h 3 with high abundance, good MS response and highly reliability were identified, which can be used as candidate peptide markers for the detection of peanut allergens in different food matrices. |
| format | Article |
| id | doaj-art-93106df280b54039b957417b29c1ecf3 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-93106df280b54039b957417b29c1ecf32025-02-03T10:34:09ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123851860Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometryXinke Yang0Haochen Bai1Lijun Yin2Jin Wang3Wentong Xue4Xin Jia5College of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing 100083, ChinaThe Key Laboratory of Crop Genetics and Breeding, Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, ChinaCollege of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing 100083, China; Corresponding author at: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, Beijing Key Laboratory of Functional Food from Plant Resources, China Agricultural University, Beijing 100083, China; Corresponding author at: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, ChinaHigh oleic-acid peanuts are known for their pre-longed shelf-life and health benefit due to high content of oleic fatty acid. However, the allergenicity and allergenic protein profiles in Chinese high-oleic peanuts have yet to be studied. For this purpose, an Orbitrap Fusion mass spectrometry (MS)-based method that is feasible for identification of putative allergenic protein as well as semi-quantitation of five major allergen protein in three different Chinese high-oleic peanut cultivars (JH 13, JH 16 and JH 18) have been reported. Results show that three Chinese high-oleic acid peanut cultivars selected all contained highly allergenic proteins Ara h 1, Ara h 2, Ara h 3 and Ara h 6. The allergenic protein profiles of Chinese high-oleic acid peanut cultivars were very similar to that of conventional peanuts, but the allergenic protein subunits varied greatly among high-oleic peanuts. Additionally, a comprehensive peptide-filtering pipeline had been developed for identification of potential peptide markers in peanut allergen proteins. Through the peptide-filtering pipeline, three novel peptide markers, IVQIEAKPNTLVLPK, SSNPDIYNPQAGSLR and AQSENYEYLAFK surrogate to Ara h 1, Ara h 3 with high abundance, good MS response and highly reliability were identified, which can be used as candidate peptide markers for the detection of peanut allergens in different food matrices.http://www.sciencedirect.com/science/article/pii/S2213453022002130AllergyHigh-oleic acid peanutPeptide-filtering pipelineNanoLC-Orbitrap mass spectrometry |
| spellingShingle | Xinke Yang Haochen Bai Lijun Yin Jin Wang Wentong Xue Xin Jia Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry Food Science and Human Wellness Allergy High-oleic acid peanut Peptide-filtering pipeline NanoLC-Orbitrap mass spectrometry |
| title | Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry |
| title_full | Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry |
| title_fullStr | Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry |
| title_full_unstemmed | Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry |
| title_short | Evaluation of allergenic protein profiles in three Chinese high-oleic acid peanut cultivars using NanoLC-Orbitrap mass spectrometry |
| title_sort | evaluation of allergenic protein profiles in three chinese high oleic acid peanut cultivars using nanolc orbitrap mass spectrometry |
| topic | Allergy High-oleic acid peanut Peptide-filtering pipeline NanoLC-Orbitrap mass spectrometry |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002130 |
| work_keys_str_mv | AT xinkeyang evaluationofallergenicproteinprofilesinthreechinesehigholeicacidpeanutcultivarsusingnanolcorbitrapmassspectrometry AT haochenbai evaluationofallergenicproteinprofilesinthreechinesehigholeicacidpeanutcultivarsusingnanolcorbitrapmassspectrometry AT lijunyin evaluationofallergenicproteinprofilesinthreechinesehigholeicacidpeanutcultivarsusingnanolcorbitrapmassspectrometry AT jinwang evaluationofallergenicproteinprofilesinthreechinesehigholeicacidpeanutcultivarsusingnanolcorbitrapmassspectrometry AT wentongxue evaluationofallergenicproteinprofilesinthreechinesehigholeicacidpeanutcultivarsusingnanolcorbitrapmassspectrometry AT xinjia evaluationofallergenicproteinprofilesinthreechinesehigholeicacidpeanutcultivarsusingnanolcorbitrapmassspectrometry |