Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst
Extracellular superoxide dismutase (SOD3), an enzyme mediating dismutation of superoxide into hydrogen peroxide, has been shown to reduce inflammation by inhibiting macrophage migration into injured tissues. In inflamed tissues, superoxide is produced by the phagocytic NOX2 complex, which consists o...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2012/730469 |
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| author | Tiina Kelkka Juha Petteri Laurila Outi Sareila Peter Olofsson Mikko Olavi Laukkanen Rikard Holmdahl |
| author_facet | Tiina Kelkka Juha Petteri Laurila Outi Sareila Peter Olofsson Mikko Olavi Laukkanen Rikard Holmdahl |
| author_sort | Tiina Kelkka |
| collection | DOAJ |
| description | Extracellular superoxide dismutase (SOD3), an enzyme mediating dismutation of superoxide into hydrogen peroxide, has been shown to reduce inflammation by inhibiting macrophage migration into injured tissues. In inflamed tissues, superoxide is produced by the phagocytic NOX2 complex, which consists of the catalytic subunit NOX2 and several regulatory subunits (e.g., NCF1). To analyze whether SOD3 can regulate inflammation in the absence of functional NOX2 complex, we injected an adenoviral vector overexpressing SOD3 directly into the arthritic paws of Ncf1*/* mice with collagen-induced arthritis. SOD3 reduced arthritis severity in both oxidative burst-deficient Ncf1*/* mice and also in wild-type mice. The NOX2 complex independent anti-inflammatory effect of SOD3 was further characterized in peritonitis, and SOD3 was found to reduce macrophage infiltration independently of NOX2 complex functionality. We conclude that the SOD3-mediated anti-inflammatory effect on arthritis and peritonitis operates independently of NOX2 complex derived oxidative burst. |
| format | Article |
| id | doaj-art-930f0fcd70da4dd889a2e2e74d508665 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-930f0fcd70da4dd889a2e2e74d5086652025-02-03T05:51:26ZengWileyMediators of Inflammation0962-93511466-18612012-01-01201210.1155/2012/730469730469Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative BurstTiina Kelkka0Juha Petteri Laurila1Outi Sareila2Peter Olofsson3Mikko Olavi Laukkanen4Rikard Holmdahl5Medicity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, FinlandMedicity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, FinlandMedicity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, FinlandRedoxis AB, Sahlgrenska Science Park, Medicinaregatan 8A, 413 46 Göteborg, SwedenMedicity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, FinlandMedicity Research Laboratory, University of Turku, Tykistökatu 6 A, 20520 Turku, FinlandExtracellular superoxide dismutase (SOD3), an enzyme mediating dismutation of superoxide into hydrogen peroxide, has been shown to reduce inflammation by inhibiting macrophage migration into injured tissues. In inflamed tissues, superoxide is produced by the phagocytic NOX2 complex, which consists of the catalytic subunit NOX2 and several regulatory subunits (e.g., NCF1). To analyze whether SOD3 can regulate inflammation in the absence of functional NOX2 complex, we injected an adenoviral vector overexpressing SOD3 directly into the arthritic paws of Ncf1*/* mice with collagen-induced arthritis. SOD3 reduced arthritis severity in both oxidative burst-deficient Ncf1*/* mice and also in wild-type mice. The NOX2 complex independent anti-inflammatory effect of SOD3 was further characterized in peritonitis, and SOD3 was found to reduce macrophage infiltration independently of NOX2 complex functionality. We conclude that the SOD3-mediated anti-inflammatory effect on arthritis and peritonitis operates independently of NOX2 complex derived oxidative burst.http://dx.doi.org/10.1155/2012/730469 |
| spellingShingle | Tiina Kelkka Juha Petteri Laurila Outi Sareila Peter Olofsson Mikko Olavi Laukkanen Rikard Holmdahl Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst Mediators of Inflammation |
| title | Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst |
| title_full | Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst |
| title_fullStr | Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst |
| title_full_unstemmed | Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst |
| title_short | Superoxide Dismutase 3 Limits Collagen-Induced Arthritis in the Absence of Phagocyte Oxidative Burst |
| title_sort | superoxide dismutase 3 limits collagen induced arthritis in the absence of phagocyte oxidative burst |
| url | http://dx.doi.org/10.1155/2012/730469 |
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