Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis
Objective. Periodontitis is an inflammatory disease of microbial etiology caused primarily by dysbiosis of the oral microbiota. Our aim was to compare variations in the composition of the oral microbiomes of youths with severe periodontitis according to gender. Methods. Subgingival plaque samples co...
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/8124593 |
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| author | Ya-Qiong Zhao Ying-Hui Zhou Jie Zhao Yao Feng Zheng-Rong Gao Qin Ye Qiong Liu Yun Chen Shao-Hui Zhang Li Tan Marie Aimee Dusenge Jing Hu Yun-Zhi Feng Fei Yan Yue Guo |
| author_facet | Ya-Qiong Zhao Ying-Hui Zhou Jie Zhao Yao Feng Zheng-Rong Gao Qin Ye Qiong Liu Yun Chen Shao-Hui Zhang Li Tan Marie Aimee Dusenge Jing Hu Yun-Zhi Feng Fei Yan Yue Guo |
| author_sort | Ya-Qiong Zhao |
| collection | DOAJ |
| description | Objective. Periodontitis is an inflammatory disease of microbial etiology caused primarily by dysbiosis of the oral microbiota. Our aim was to compare variations in the composition of the oral microbiomes of youths with severe periodontitis according to gender. Methods. Subgingival plaque samples collected from 17 patients with severe periodontitis (11 males and 6 females) were split for 16S rRNA gene sequencing. The composition, α-diversity, and β-diversity of the patients’ oral microbiomes were compared between the males and the females. Linear discriminant analysis effect size (LEfSe) was used to analyze the specific taxa enriched in the two groups. Functional profiles (KEGG pathways) were obtained using PICRUSt based on 16S rRNA gene sequencing data. Results. The Chao1 index and phylogenetic diversity whole tree were significantly higher in males than in females. The Simpson and Shannon indices were not significantly different between the two groups. β-Diversity suggested that the samples were reasonably divided into groups. The Kruskal-Wallis test based on the relative abundance of species, combined with the LEfSe analysis showed that the dominant bacteria in males were Pseudomonas and Papillibacter, whereas the dominant bacteria in women were Fusobacteriales and Tannerella. KEGG analysis predicted that the variation in the oral microbiome may be related to the immune system in women, whereas immune system diseases were the dominant pathway in men. Conclusion. We found sex-specific differences in the oral microbiome in a sample of youths with severe periodontitis. The differences may be related to changes in immune homeostasis and lead to a better understanding of periodontitis. |
| format | Article |
| id | doaj-art-9304d4cdcac449ed91077461e4a73280 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-9304d4cdcac449ed91077461e4a732802025-02-03T01:25:48ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/81245938124593Sex Variations in the Oral Microbiomes of Youths with Severe PeriodontitisYa-Qiong Zhao0Ying-Hui Zhou1Jie Zhao2Yao Feng3Zheng-Rong Gao4Qin Ye5Qiong Liu6Yun Chen7Shao-Hui Zhang8Li Tan9Marie Aimee Dusenge10Jing Hu11Yun-Zhi Feng12Fei Yan13Yue Guo14Department of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaNational Clinical Research Center for Metabolic Diseases, Hunan Provincial Key Laboratory of Metabolic Bone Diseases, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaHunan Key Laboratory of Oral Health Research & Hunan 3D Printing Engineering Research Center of Oral Care & Hunan Clinical Research Center of Oral Major Diseases and Oral Health & Xiangya Stomatological Hospital & Xiangya School of Stomatology, Central South University, Changsha 410008, ChinaDepartment of Stomatology, The Second Xiangya Hospital of Central South University, Changsha 410011, Hunan, ChinaObjective. Periodontitis is an inflammatory disease of microbial etiology caused primarily by dysbiosis of the oral microbiota. Our aim was to compare variations in the composition of the oral microbiomes of youths with severe periodontitis according to gender. Methods. Subgingival plaque samples collected from 17 patients with severe periodontitis (11 males and 6 females) were split for 16S rRNA gene sequencing. The composition, α-diversity, and β-diversity of the patients’ oral microbiomes were compared between the males and the females. Linear discriminant analysis effect size (LEfSe) was used to analyze the specific taxa enriched in the two groups. Functional profiles (KEGG pathways) were obtained using PICRUSt based on 16S rRNA gene sequencing data. Results. The Chao1 index and phylogenetic diversity whole tree were significantly higher in males than in females. The Simpson and Shannon indices were not significantly different between the two groups. β-Diversity suggested that the samples were reasonably divided into groups. The Kruskal-Wallis test based on the relative abundance of species, combined with the LEfSe analysis showed that the dominant bacteria in males were Pseudomonas and Papillibacter, whereas the dominant bacteria in women were Fusobacteriales and Tannerella. KEGG analysis predicted that the variation in the oral microbiome may be related to the immune system in women, whereas immune system diseases were the dominant pathway in men. Conclusion. We found sex-specific differences in the oral microbiome in a sample of youths with severe periodontitis. The differences may be related to changes in immune homeostasis and lead to a better understanding of periodontitis.http://dx.doi.org/10.1155/2021/8124593 |
| spellingShingle | Ya-Qiong Zhao Ying-Hui Zhou Jie Zhao Yao Feng Zheng-Rong Gao Qin Ye Qiong Liu Yun Chen Shao-Hui Zhang Li Tan Marie Aimee Dusenge Jing Hu Yun-Zhi Feng Fei Yan Yue Guo Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis Journal of Immunology Research |
| title | Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis |
| title_full | Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis |
| title_fullStr | Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis |
| title_full_unstemmed | Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis |
| title_short | Sex Variations in the Oral Microbiomes of Youths with Severe Periodontitis |
| title_sort | sex variations in the oral microbiomes of youths with severe periodontitis |
| url | http://dx.doi.org/10.1155/2021/8124593 |
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