Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells
An artificial HIV-1 enhancer-binding peptide was extended by nine consecutive arginine residues at the C-terminus and by the nuclear localization signal of SV40 large T antigen at the N-terminus. The resulting synthetic 64-residue peptide was found to bind to the two enhancers of the HIV-1 long term...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2011/165871 |
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| _version_ | 1832554099957039104 |
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| author | Mouhssin Oufir Leslie R. Bisset Stefan R. K. Hoffmann Gongda Xue Stephan Klauser Bianca Bergamaschi Alain Gervaix Jürg Böni Jörg Schüpbach Bernd Gutte |
| author_facet | Mouhssin Oufir Leslie R. Bisset Stefan R. K. Hoffmann Gongda Xue Stephan Klauser Bianca Bergamaschi Alain Gervaix Jürg Böni Jörg Schüpbach Bernd Gutte |
| author_sort | Mouhssin Oufir |
| collection | DOAJ |
| description | An artificial HIV-1 enhancer-binding peptide was extended by nine consecutive arginine residues at the C-terminus and by the nuclear localization signal of SV40 large T antigen at the N-terminus. The resulting synthetic 64-residue peptide was found to bind to the two enhancers of the HIV-1 long terminal repeat, cross the plasma membrane and the nuclear envelope of human cells, and suppress the HIV-1 enhancer-controlled expression of a green fluorescent protein reporter gene. Moreover, HIV-1 replication is inhibited by this peptide in HIV-1-infected CEM-GFP cells as revealed by HIV-1 p24 ELISA and real-time RT-PCR of HIV-1 RNA. Rapid uptake of this intracellular stable and inhibitory peptide into the cells implies that this peptide may have the potential to attenuate HIV-1 replication in vivo. |
| format | Article |
| id | doaj-art-92fc98ba0c004ad3bd069b5d19189400 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-92fc98ba0c004ad3bd069b5d191894002025-02-03T05:52:22ZengWileyAdvances in Virology1687-86391687-86472011-01-01201110.1155/2011/165871165871Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected CellsMouhssin Oufir0Leslie R. Bisset1Stefan R. K. Hoffmann2Gongda Xue3Stephan Klauser4Bianca Bergamaschi5Alain Gervaix6Jürg Böni7Jörg Schüpbach8Bernd Gutte9Biochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandSwiss National Center for Retroviruses, Institute for Medical Virology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandBiochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandBiochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandBiochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandBiochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandDépartement de Pédiatrie, Hôpital des Enfants HUG, CH-1211 Genève, SwitzerlandSwiss National Center for Retroviruses, Institute for Medical Virology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandSwiss National Center for Retroviruses, Institute for Medical Virology, University of Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandBiochemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, SwitzerlandAn artificial HIV-1 enhancer-binding peptide was extended by nine consecutive arginine residues at the C-terminus and by the nuclear localization signal of SV40 large T antigen at the N-terminus. The resulting synthetic 64-residue peptide was found to bind to the two enhancers of the HIV-1 long terminal repeat, cross the plasma membrane and the nuclear envelope of human cells, and suppress the HIV-1 enhancer-controlled expression of a green fluorescent protein reporter gene. Moreover, HIV-1 replication is inhibited by this peptide in HIV-1-infected CEM-GFP cells as revealed by HIV-1 p24 ELISA and real-time RT-PCR of HIV-1 RNA. Rapid uptake of this intracellular stable and inhibitory peptide into the cells implies that this peptide may have the potential to attenuate HIV-1 replication in vivo.http://dx.doi.org/10.1155/2011/165871 |
| spellingShingle | Mouhssin Oufir Leslie R. Bisset Stefan R. K. Hoffmann Gongda Xue Stephan Klauser Bianca Bergamaschi Alain Gervaix Jürg Böni Jörg Schüpbach Bernd Gutte Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells Advances in Virology |
| title | Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells |
| title_full | Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells |
| title_fullStr | Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells |
| title_full_unstemmed | Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells |
| title_short | Artificial 64-Residue HIV-1 Enhancer-Binding Peptide Is a Potent Inhibitor of Viral Replication in HIV-1-Infected Cells |
| title_sort | artificial 64 residue hiv 1 enhancer binding peptide is a potent inhibitor of viral replication in hiv 1 infected cells |
| url | http://dx.doi.org/10.1155/2011/165871 |
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