Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67
ABSTRACT Background: To compare Gleason 7 (3+4) and (4+3) prostatic adenocarcinoma (PC) with different prognostic criteria through immunohistochemical analysis with anti-PSA, anti-Ki 67 and anti-AMARC antibodies. Methods: We analyzed 221 surgical specimens from patients between 40 and 86 years-old...
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Sociedade Brasileira de Urologia
2025-01-01
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| Series: | International Brazilian Journal of Urology |
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| author | Clarice F. E. M. Osório Waldemar S. Costa Carla B. M. Gallo Luciano A. Favorito Francisco J. B. Sampaio |
| author_facet | Clarice F. E. M. Osório Waldemar S. Costa Carla B. M. Gallo Luciano A. Favorito Francisco J. B. Sampaio |
| author_sort | Clarice F. E. M. Osório |
| collection | DOAJ |
| description | ABSTRACT Background: To compare Gleason 7 (3+4) and (4+3) prostatic adenocarcinoma (PC) with different prognostic criteria through immunohistochemical analysis with anti-PSA, anti-Ki 67 and anti-AMARC antibodies. Methods: We analyzed 221 surgical specimens from patients between 40 and 86 years-old (mean=63) with PC. The immunohistochemical study was performed with anti-PSA, anti-Ki 67 and anti-AMARC. The microscopic fields were photographed with an Olympus DP70 digital camera coupled to an Olympus BX51 microscope and archived in TIFF. Proportion and intensity criteria were used to quantify the anti-PSA antibody and for the anti-Ki 67 antibody, the quantification by similarity of this antibody in breast carcinomas. Anti-AMACR protein expression was based on four scores: negative, weak, moderate and strong. The statistical analysis was performed with the Graph Pad Prism 5 program. Results: In the Gleason score 7 (3+4) we had 91.72% in pT2 and 8.27% in the pT3 group; 8.27% recurrences, of which 90.90% in the pT2 group. In the Gleason score 7 (4+3) we had 77.27% in the pT2 group and 22.72% in the pT3 group and 10.22% of relapses, of which 66.66% in the pT2 group and 33.33% in the pT3 group. In 6.81% of cases there was an increase in the anti-Ki 67 index and in 2.27% of the cases, there was an increase in the immunoexpression of anti-p53 when comparing Gleason score 7 (3+4) with Gleason score 7 (4+3). Conclusion: Our study confirmed differences in the Gleason score 7 (3+4) and Gleason score 7 (4+3) of PC when comparing prognostic criteria. Anti-Ki 67 and anti-PSA antibody immunostaining showed a positive correlation as the Gleason score 7 increased from (3+4) to (4+3). |
| format | Article |
| id | doaj-art-92f3f0cbe8354838af49c88fb6992845 |
| institution | Kabale University |
| issn | 1677-6119 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Sociedade Brasileira de Urologia |
| record_format | Article |
| series | International Brazilian Journal of Urology |
| spelling | doaj-art-92f3f0cbe8354838af49c88fb69928452025-02-04T07:37:16ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-61192025-01-0151110.1590/s1677-5538.ibju.2024.9922Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67Clarice F. E. M. OsórioWaldemar S. CostaCarla B. M. GalloLuciano A. FavoritoFrancisco J. B. Sampaiohttps://orcid.org/0000-0001-9087-9319ABSTRACT Background: To compare Gleason 7 (3+4) and (4+3) prostatic adenocarcinoma (PC) with different prognostic criteria through immunohistochemical analysis with anti-PSA, anti-Ki 67 and anti-AMARC antibodies. Methods: We analyzed 221 surgical specimens from patients between 40 and 86 years-old (mean=63) with PC. The immunohistochemical study was performed with anti-PSA, anti-Ki 67 and anti-AMARC. The microscopic fields were photographed with an Olympus DP70 digital camera coupled to an Olympus BX51 microscope and archived in TIFF. Proportion and intensity criteria were used to quantify the anti-PSA antibody and for the anti-Ki 67 antibody, the quantification by similarity of this antibody in breast carcinomas. Anti-AMACR protein expression was based on four scores: negative, weak, moderate and strong. The statistical analysis was performed with the Graph Pad Prism 5 program. Results: In the Gleason score 7 (3+4) we had 91.72% in pT2 and 8.27% in the pT3 group; 8.27% recurrences, of which 90.90% in the pT2 group. In the Gleason score 7 (4+3) we had 77.27% in the pT2 group and 22.72% in the pT3 group and 10.22% of relapses, of which 66.66% in the pT2 group and 33.33% in the pT3 group. In 6.81% of cases there was an increase in the anti-Ki 67 index and in 2.27% of the cases, there was an increase in the immunoexpression of anti-p53 when comparing Gleason score 7 (3+4) with Gleason score 7 (4+3). Conclusion: Our study confirmed differences in the Gleason score 7 (3+4) and Gleason score 7 (4+3) of PC when comparing prognostic criteria. Anti-Ki 67 and anti-PSA antibody immunostaining showed a positive correlation as the Gleason score 7 increased from (3+4) to (4+3).http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382025000100405&lng=en&tlng=enProstatic NeoplasmsNeoplasm GradingPrognosisHistological Techniques |
| spellingShingle | Clarice F. E. M. Osório Waldemar S. Costa Carla B. M. Gallo Luciano A. Favorito Francisco J. B. Sampaio Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67 International Brazilian Journal of Urology Prostatic Neoplasms Neoplasm Grading Prognosis Histological Techniques |
| title | Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67 |
| title_full | Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67 |
| title_fullStr | Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67 |
| title_full_unstemmed | Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67 |
| title_short | Comparative Study of Gleason 7 (3+4) and (4+3) Prostatic Adenocarcinomas with Prognostic Criteria and Immunohistochemical Profiles of AMACR, PSA and Ki-67 |
| title_sort | comparative study of gleason 7 3 4 and 4 3 prostatic adenocarcinomas with prognostic criteria and immunohistochemical profiles of amacr psa and ki 67 |
| topic | Prostatic Neoplasms Neoplasm Grading Prognosis Histological Techniques |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382025000100405&lng=en&tlng=en |
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