The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance

Abstract Background Cyperus esculentus L. var. sativus (chufa) is a perennial plant known for its nutrient-rich underground tubers and aboveground leaves, which offer significant food and health advantages worldwide. Despite its benefits, salt stress hinders plant growth, thus limiting both yield an...

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Main Authors: Saisai Wang, Yanna Huang, Xueming Tang
Format: Article
Language:English
Published: BMC 2025-04-01
Series:BMC Plant Biology
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Online Access:https://doi.org/10.1186/s12870-025-06562-2
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author Saisai Wang
Yanna Huang
Xueming Tang
author_facet Saisai Wang
Yanna Huang
Xueming Tang
author_sort Saisai Wang
collection DOAJ
description Abstract Background Cyperus esculentus L. var. sativus (chufa) is a perennial plant known for its nutrient-rich underground tubers and aboveground leaves, which offer significant food and health advantages worldwide. Despite its benefits, salt stress hinders plant growth, thus limiting both yield and quality. While the positive effects of plant growth-promoting endophytes (PGPEs) on plant growth have been established, their particular influence on chufa under salt stress has yet to be investigated. This research focused on examining the principal physiological and metabolic responses of chufa following inoculation with the PGPE Franconibacter sp. YSD YN2 at varying NaCl concentrations. Results YSD YN2 exhibited significant salt resistance, robust colonization of plant compartments, and various characteristics (indole-3-acetic acid (IAA) (0.90–14.74 µg/mL), phosphate (P) (0.00–20.00 µg/mL), potassium (K) solubility (1.00–2.50), and exopolysaccharides (EPSs) (0.80–28.09 mg/mL)) under different NaCl concentrations, underscoring its capacity to enhance plant development and recuperation under salt stress. YSD YN2 inoculation significantly enhanced plant growth across different NaCl concentrations, including improvements in shoot height (13.33–26.67%), shoot weight (48.91–115.38%), root length (7.84–13.17%), root weight (39.43–63.06%), relative water content (1.78–10.80%), and the number of tillers (50.00–183.50%). Inoculation with YSD YN2 resulted in increased total chlorophyll contents (25.30–83.57%), improved efficiency of antioxidant enzymes such as peroxidase (POD) (13.81–35.48 fold), superoxide dismutase (SOD) (10.67–13.34%), and catalase (CAT) (25.00–78.95%), diminished malondialdehyde (MDA) accumulation (14.10–31.19%), and increased proline accumulation (11.37–19.64%) in chufa. Additionally, inoculation with YSD YN2 led to a reduction in internal Na+ accumulation and an increase in K+ and Ca2+ absorption. Conclusion YSD YN2 may serve as a valuable PGPE for improving plant development, photosynthetic pigmentation, oxidative processes, and osmotic control substances, controlling ion absorption, and reducing the harmful impacts of high-salinity chufa.
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spelling doaj-art-92cf2e1aa9cf4c8cad9cc986f7d8f93a2025-08-20T02:55:38ZengBMCBMC Plant Biology1471-22292025-04-0125111510.1186/s12870-025-06562-2The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt toleranceSaisai Wang0Yanna Huang1Xueming Tang2School of Agriculture and Biology, Shanghai Jiao Tong UniversitySchool of Agriculture and Biology, Shanghai Jiao Tong UniversitySchool of Agriculture and Biology, Shanghai Jiao Tong UniversityAbstract Background Cyperus esculentus L. var. sativus (chufa) is a perennial plant known for its nutrient-rich underground tubers and aboveground leaves, which offer significant food and health advantages worldwide. Despite its benefits, salt stress hinders plant growth, thus limiting both yield and quality. While the positive effects of plant growth-promoting endophytes (PGPEs) on plant growth have been established, their particular influence on chufa under salt stress has yet to be investigated. This research focused on examining the principal physiological and metabolic responses of chufa following inoculation with the PGPE Franconibacter sp. YSD YN2 at varying NaCl concentrations. Results YSD YN2 exhibited significant salt resistance, robust colonization of plant compartments, and various characteristics (indole-3-acetic acid (IAA) (0.90–14.74 µg/mL), phosphate (P) (0.00–20.00 µg/mL), potassium (K) solubility (1.00–2.50), and exopolysaccharides (EPSs) (0.80–28.09 mg/mL)) under different NaCl concentrations, underscoring its capacity to enhance plant development and recuperation under salt stress. YSD YN2 inoculation significantly enhanced plant growth across different NaCl concentrations, including improvements in shoot height (13.33–26.67%), shoot weight (48.91–115.38%), root length (7.84–13.17%), root weight (39.43–63.06%), relative water content (1.78–10.80%), and the number of tillers (50.00–183.50%). Inoculation with YSD YN2 resulted in increased total chlorophyll contents (25.30–83.57%), improved efficiency of antioxidant enzymes such as peroxidase (POD) (13.81–35.48 fold), superoxide dismutase (SOD) (10.67–13.34%), and catalase (CAT) (25.00–78.95%), diminished malondialdehyde (MDA) accumulation (14.10–31.19%), and increased proline accumulation (11.37–19.64%) in chufa. Additionally, inoculation with YSD YN2 led to a reduction in internal Na+ accumulation and an increase in K+ and Ca2+ absorption. Conclusion YSD YN2 may serve as a valuable PGPE for improving plant development, photosynthetic pigmentation, oxidative processes, and osmotic control substances, controlling ion absorption, and reducing the harmful impacts of high-salinity chufa.https://doi.org/10.1186/s12870-025-06562-2ChufaSalt stressPGPEPhysiologicalmetabolic responsesFranconibacter Sp. YSD YN2
spellingShingle Saisai Wang
Yanna Huang
Xueming Tang
The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance
BMC Plant Biology
Chufa
Salt stress
PGPE
Physiological
metabolic responses
Franconibacter Sp. YSD YN2
title The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance
title_full The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance
title_fullStr The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance
title_full_unstemmed The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance
title_short The role of endophytic salt-tolerant Franconibacter Sp. YSD YN2 in Cyperus esculentus L. Var sativus: impacts on plant growth and mechanisms of salt tolerance
title_sort role of endophytic salt tolerant franconibacter sp ysd yn2 in cyperus esculentus l var sativus impacts on plant growth and mechanisms of salt tolerance
topic Chufa
Salt stress
PGPE
Physiological
metabolic responses
Franconibacter Sp. YSD YN2
url https://doi.org/10.1186/s12870-025-06562-2
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