Cation Involvement in Telomestatin Binding to G-Quadruplex DNA
The binding mode of telomestatin to G-quadruplex DNA has been investigated using electrospray mass spectrometry, by detecting the intact complexes formed in ammonium acetate. The mass measurements show the incorporation of one extra ammonium ion in the telomestatin complexes. Experiments on telomest...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/121259 |
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| _version_ | 1832548412216573952 |
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| author | Frédéric Rosu Valérie Gabelica Nicolas Smargiasso Gabriel Mazzucchelli Kazuo Shin-Ya Edwin De Pauw |
| author_facet | Frédéric Rosu Valérie Gabelica Nicolas Smargiasso Gabriel Mazzucchelli Kazuo Shin-Ya Edwin De Pauw |
| author_sort | Frédéric Rosu |
| collection | DOAJ |
| description | The binding mode of telomestatin to G-quadruplex DNA has been investigated using electrospray mass spectrometry, by detecting the intact complexes formed in ammonium acetate. The mass measurements show the incorporation of one extra ammonium ion in the telomestatin complexes. Experiments on telomestatin alone also show that the telomestatin alone is able to coordinate cations in a similar way as a crown ether. Finally, density functional theory calculations suggest that in the G-quadruplex-telomestatin complex, potassium or ammonium cations are located between the telomestatin and a G-quartet. This study underlines that monovalent cation coordination capabilities should be integrated in the rational design of G-quadruplex binding ligands. |
| format | Article |
| id | doaj-art-92bfacb45c2d4066a9b7a895cd498c1f |
| institution | Kabale University |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-92bfacb45c2d4066a9b7a895cd498c1f2025-02-03T06:14:11ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/121259121259Cation Involvement in Telomestatin Binding to G-Quadruplex DNAFrédéric Rosu0Valérie Gabelica1Nicolas Smargiasso2Gabriel Mazzucchelli3Kazuo Shin-Ya4Edwin De Pauw5Mass Spectrometry Laboratory, Department of Chemistry, University of Liège, 4000 Liège, BelgiumMass Spectrometry Laboratory, Department of Chemistry, University of Liège, 4000 Liège, BelgiumMass Spectrometry Laboratory, Department of Chemistry, University of Liège, 4000 Liège, BelgiumMass Spectrometry Laboratory, Department of Chemistry, University of Liège, 4000 Liège, BelgiumBiomedicinal Information Research Center, Biological Systems Control Team, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, JapanMass Spectrometry Laboratory, Department of Chemistry, University of Liège, 4000 Liège, BelgiumThe binding mode of telomestatin to G-quadruplex DNA has been investigated using electrospray mass spectrometry, by detecting the intact complexes formed in ammonium acetate. The mass measurements show the incorporation of one extra ammonium ion in the telomestatin complexes. Experiments on telomestatin alone also show that the telomestatin alone is able to coordinate cations in a similar way as a crown ether. Finally, density functional theory calculations suggest that in the G-quadruplex-telomestatin complex, potassium or ammonium cations are located between the telomestatin and a G-quartet. This study underlines that monovalent cation coordination capabilities should be integrated in the rational design of G-quadruplex binding ligands.http://dx.doi.org/10.4061/2010/121259 |
| spellingShingle | Frédéric Rosu Valérie Gabelica Nicolas Smargiasso Gabriel Mazzucchelli Kazuo Shin-Ya Edwin De Pauw Cation Involvement in Telomestatin Binding to G-Quadruplex DNA Journal of Nucleic Acids |
| title | Cation Involvement in Telomestatin Binding to G-Quadruplex DNA |
| title_full | Cation Involvement in Telomestatin Binding to G-Quadruplex DNA |
| title_fullStr | Cation Involvement in Telomestatin Binding to G-Quadruplex DNA |
| title_full_unstemmed | Cation Involvement in Telomestatin Binding to G-Quadruplex DNA |
| title_short | Cation Involvement in Telomestatin Binding to G-Quadruplex DNA |
| title_sort | cation involvement in telomestatin binding to g quadruplex dna |
| url | http://dx.doi.org/10.4061/2010/121259 |
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