Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis
Copper is an essential micro-element in animal growth. Tribasic copper chloride (TBCC), as an important source of animal feed copper, is widely used in agricultural production. Multiple studies have shown that excessive intake of copper can affect the immune function of animals and lead to disease o...
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324014994 |
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| author | Jinwen Quan Xiaoyue Chang Siying Liu Ting He Gaolong Zhong Zhonghua Liu Wenlan Yu |
| author_facet | Jinwen Quan Xiaoyue Chang Siying Liu Ting He Gaolong Zhong Zhonghua Liu Wenlan Yu |
| author_sort | Jinwen Quan |
| collection | DOAJ |
| description | Copper is an essential micro-element in animal growth. Tribasic copper chloride (TBCC), as an important source of animal feed copper, is widely used in agricultural production. Multiple studies have shown that excessive intake of copper can affect the immune function of animals and lead to disease or death. The intestine-spleen axis, which is the interaction between the spleen, intestines and gut microbiota, is closely linked to immune function in the body. However, the mechanism of intestine-spleen axis affecting TBCC induced immune dysfunction in rats has not been revealed. In this study, we analyzed the impacts of different doses of TBCC on the intestine, gut microbiota and spleen of rats, and investigated their relationship in the process. Our results demonstrated that under copper exposure, the structural integrity of the intestinal epithelium was compromised, resulting in a significant downregulation of genes and proteins expression levels related to the intestinal barrier (Zonula occludens-1, Claudin-1 and Occludin). Additionally, copper perturbed the composition of the rat intestinal microbiota, altered the abundance and diversity of the microbial community. Copper entered the spleen via the intestine-spleen axis, leading to structural damage in the spleen and activation of the NFκB signaling pathway, and increased pro-inflammatory cytokines (IFN-γ, TNF-α, IL-18, and IL-1β), ultimately causing pyroptosis and inflammation in the spleen. These findings offer a novel perspective on how copper may induce spleen injury through the intestine-spleen axis. |
| format | Article |
| id | doaj-art-92bb536cbd874e3197b1f70ae44b96ca |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-92bb536cbd874e3197b1f70ae44b96ca2025-01-23T05:25:29ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01289117423Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axisJinwen Quan0Xiaoyue Chang1Siying Liu2Ting He3Gaolong Zhong4Zhonghua Liu5Wenlan Yu6College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Laboratory Animal Center, South China Agriculture University, Guangzhou, Guangdong 510642, PR ChinaCollege of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR China; Laboratory Animal Center, South China Agriculture University, Guangzhou, Guangdong 510642, PR China; Corresponding author at: College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, PR China.Copper is an essential micro-element in animal growth. Tribasic copper chloride (TBCC), as an important source of animal feed copper, is widely used in agricultural production. Multiple studies have shown that excessive intake of copper can affect the immune function of animals and lead to disease or death. The intestine-spleen axis, which is the interaction between the spleen, intestines and gut microbiota, is closely linked to immune function in the body. However, the mechanism of intestine-spleen axis affecting TBCC induced immune dysfunction in rats has not been revealed. In this study, we analyzed the impacts of different doses of TBCC on the intestine, gut microbiota and spleen of rats, and investigated their relationship in the process. Our results demonstrated that under copper exposure, the structural integrity of the intestinal epithelium was compromised, resulting in a significant downregulation of genes and proteins expression levels related to the intestinal barrier (Zonula occludens-1, Claudin-1 and Occludin). Additionally, copper perturbed the composition of the rat intestinal microbiota, altered the abundance and diversity of the microbial community. Copper entered the spleen via the intestine-spleen axis, leading to structural damage in the spleen and activation of the NFκB signaling pathway, and increased pro-inflammatory cytokines (IFN-γ, TNF-α, IL-18, and IL-1β), ultimately causing pyroptosis and inflammation in the spleen. These findings offer a novel perspective on how copper may induce spleen injury through the intestine-spleen axis.http://www.sciencedirect.com/science/article/pii/S0147651324014994RatCopperIntestinal microbiotaIntestine-spleen axisPyroptosis |
| spellingShingle | Jinwen Quan Xiaoyue Chang Siying Liu Ting He Gaolong Zhong Zhonghua Liu Wenlan Yu Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis Ecotoxicology and Environmental Safety Rat Copper Intestinal microbiota Intestine-spleen axis Pyroptosis |
| title | Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis |
| title_full | Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis |
| title_fullStr | Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis |
| title_full_unstemmed | Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis |
| title_short | Long-term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal-splenic axis |
| title_sort | long term copper exposure induced pyroptosis and inflammation of rat spleen through intestinal splenic axis |
| topic | Rat Copper Intestinal microbiota Intestine-spleen axis Pyroptosis |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324014994 |
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