Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane
We report a low-cost, portable biosensor composed of an aptamer-functionalized nanoporous anodic aluminum oxide (NAAO) membrane and a commercial microcontroller chip-based impedance reader suitable for electrochemical impedance spectroscopy (EIS)-based sensing. The biosensor consists of two chambers...
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| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/16/1/35 |
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| author | Nianyu Jiang Pranav Shrotriya |
| author_facet | Nianyu Jiang Pranav Shrotriya |
| author_sort | Nianyu Jiang |
| collection | DOAJ |
| description | We report a low-cost, portable biosensor composed of an aptamer-functionalized nanoporous anodic aluminum oxide (NAAO) membrane and a commercial microcontroller chip-based impedance reader suitable for electrochemical impedance spectroscopy (EIS)-based sensing. The biosensor consists of two chambers separated by an aptamer-functionalized NAAO membrane, and the impedance reader is utilized to monitor transmembrane impedance changes. The biosensor is utilized to detect amodiaquine molecules using an amodiaquine-binding aptamer (OR7)-functionalized membrane. The aptamer-functionalized membrane is exposed to different concentrations of amodiaquine molecules to characterize the sensitivity of the sensor response. The specificity of the sensor response is characterized by exposure to varying concentrations of chloroquine, which is similar in structure to amodiaquine but does not bind to the OR7 aptamer. A commercial potentiostat is also used to measure the sensor response for amodiaquine and chloroquine. The sensing response measured using both the portable impedance reader and the commercial potentiostat showed a similar dynamic response and detection threshold. The specific and sensitive sensing results for amodiaquine demonstrate the efficacy of the low-cost and portable biosensor. |
| format | Article |
| id | doaj-art-92a1bcfcaa1a40c580eb7239a3f42e96 |
| institution | Kabale University |
| issn | 2072-666X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj-art-92a1bcfcaa1a40c580eb7239a3f42e962025-01-24T13:41:54ZengMDPI AGMicromachines2072-666X2024-12-011613510.3390/mi16010035Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide MembraneNianyu Jiang0Pranav Shrotriya1Ames National Laboratory, Mechanical Engineering Department, Iowa State University, Ames, IA 50014, USAAmes National Laboratory, Mechanical Engineering Department, Iowa State University, Ames, IA 50014, USAWe report a low-cost, portable biosensor composed of an aptamer-functionalized nanoporous anodic aluminum oxide (NAAO) membrane and a commercial microcontroller chip-based impedance reader suitable for electrochemical impedance spectroscopy (EIS)-based sensing. The biosensor consists of two chambers separated by an aptamer-functionalized NAAO membrane, and the impedance reader is utilized to monitor transmembrane impedance changes. The biosensor is utilized to detect amodiaquine molecules using an amodiaquine-binding aptamer (OR7)-functionalized membrane. The aptamer-functionalized membrane is exposed to different concentrations of amodiaquine molecules to characterize the sensitivity of the sensor response. The specificity of the sensor response is characterized by exposure to varying concentrations of chloroquine, which is similar in structure to amodiaquine but does not bind to the OR7 aptamer. A commercial potentiostat is also used to measure the sensor response for amodiaquine and chloroquine. The sensing response measured using both the portable impedance reader and the commercial potentiostat showed a similar dynamic response and detection threshold. The specific and sensitive sensing results for amodiaquine demonstrate the efficacy of the low-cost and portable biosensor.https://www.mdpi.com/2072-666X/16/1/35malariaamodiaquine sensingpoint-of-care sensorlow-cost impedance monitoringaptamerspoint-of-care biosensor |
| spellingShingle | Nianyu Jiang Pranav Shrotriya Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane Micromachines malaria amodiaquine sensing point-of-care sensor low-cost impedance monitoring aptamers point-of-care biosensor |
| title | Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane |
| title_full | Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane |
| title_fullStr | Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane |
| title_full_unstemmed | Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane |
| title_short | Low-Cost and Portable Biosensor Based on Monitoring Impedance Changes in Aptamer-Functionalized Nanoporous Anodized Aluminum Oxide Membrane |
| title_sort | low cost and portable biosensor based on monitoring impedance changes in aptamer functionalized nanoporous anodized aluminum oxide membrane |
| topic | malaria amodiaquine sensing point-of-care sensor low-cost impedance monitoring aptamers point-of-care biosensor |
| url | https://www.mdpi.com/2072-666X/16/1/35 |
| work_keys_str_mv | AT nianyujiang lowcostandportablebiosensorbasedonmonitoringimpedancechangesinaptamerfunctionalizednanoporousanodizedaluminumoxidemembrane AT pranavshrotriya lowcostandportablebiosensorbasedonmonitoringimpedancechangesinaptamerfunctionalizednanoporousanodizedaluminumoxidemembrane |