Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study
Objective. To determine the in vitro antibacterial effect of four concentrations of the hydroalcoholic extract of Solanum tuberosum “tocosh” (HET) against Streptococcus mutans ATCC 25175TM. Methods. This was a prospective, experimental, comparative study. Fermented tocosh was subjected to hydric str...
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| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8856382 |
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| author | Silvana Enciso Julia Medina Franco Mauricio Cesar Mauricio-Vilchez Daniel Alvitez-Temoche Luzmila Vilchez Frank Mayta-Tovalino |
| author_facet | Silvana Enciso Julia Medina Franco Mauricio Cesar Mauricio-Vilchez Daniel Alvitez-Temoche Luzmila Vilchez Frank Mayta-Tovalino |
| author_sort | Silvana Enciso |
| collection | DOAJ |
| description | Objective. To determine the in vitro antibacterial effect of four concentrations of the hydroalcoholic extract of Solanum tuberosum “tocosh” (HET) against Streptococcus mutans ATCC 25175TM. Methods. This was a prospective, experimental, comparative study. Fermented tocosh was subjected to hydric stress to obtain a hydroalcoholic extract at four different concentrations: 100%, 50%, 75%, and 25%. S. mutans strains were cultured in brain heart infusion agar using the swab technique. The antibacterial effectiveness of HET was evaluated following the Kirby–Bauer disk diffusion method and compared with 0.12% chlorhexidine (positive control group). Results. The highest mean inhibitory effect was achieved with HET at 100% (33.1 ± 2.2 mm, showing a gradual reduction in the other HET groups at 75%, 50%, and 25% (29.7 ± 1.3 mm, 26.6 ± 2.0, and 20.1 ± 1.8 mm, respectively)). Inferential analysis found statistically significant differences among all the experimental groups (p=0.001). The post hoc analysis also showed significant differences among all the experimental groups evaluated; however, there were no significant differences between HET 50% and chlorhexidine 0.12% (p>0.05). Conclusions. It was found that the highest antibacterial effectiveness was obtained by HET 100%, being even higher than the 0.12% chlorhexidine positive control, and was statistically significant. Post hoc analysis showed that almost all the concentrations showed optimal efficacy against S. mutans. |
| format | Article |
| id | doaj-art-9287fa5e048d4ffdaab73d464e9383c8 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-9287fa5e048d4ffdaab73d464e9383c82025-02-03T06:06:53ZengWileyInternational Journal of Dentistry1687-87281687-87362020-01-01202010.1155/2020/88563828856382Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro StudySilvana Enciso0Julia Medina1Franco Mauricio2Cesar Mauricio-Vilchez3Daniel Alvitez-Temoche4Luzmila Vilchez5Frank Mayta-Tovalino6Academic Department, Universidad Nacional Federico Villarreal, Lima, PeruAcademic Department, Universidad Nacional Federico Villarreal, Lima, PeruAcademic Department, Universidad Nacional Federico Villarreal, Lima, PeruAcademic Department, Universidad Nacional Federico Villarreal, Lima, PeruSchool of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, PeruAcademic Department, Universidad Nacional Federico Villarreal, Lima, PeruSchool of Dentistry, Universidad Nacional Mayor de San Marcos, Lima, PeruObjective. To determine the in vitro antibacterial effect of four concentrations of the hydroalcoholic extract of Solanum tuberosum “tocosh” (HET) against Streptococcus mutans ATCC 25175TM. Methods. This was a prospective, experimental, comparative study. Fermented tocosh was subjected to hydric stress to obtain a hydroalcoholic extract at four different concentrations: 100%, 50%, 75%, and 25%. S. mutans strains were cultured in brain heart infusion agar using the swab technique. The antibacterial effectiveness of HET was evaluated following the Kirby–Bauer disk diffusion method and compared with 0.12% chlorhexidine (positive control group). Results. The highest mean inhibitory effect was achieved with HET at 100% (33.1 ± 2.2 mm, showing a gradual reduction in the other HET groups at 75%, 50%, and 25% (29.7 ± 1.3 mm, 26.6 ± 2.0, and 20.1 ± 1.8 mm, respectively)). Inferential analysis found statistically significant differences among all the experimental groups (p=0.001). The post hoc analysis also showed significant differences among all the experimental groups evaluated; however, there were no significant differences between HET 50% and chlorhexidine 0.12% (p>0.05). Conclusions. It was found that the highest antibacterial effectiveness was obtained by HET 100%, being even higher than the 0.12% chlorhexidine positive control, and was statistically significant. Post hoc analysis showed that almost all the concentrations showed optimal efficacy against S. mutans.http://dx.doi.org/10.1155/2020/8856382 |
| spellingShingle | Silvana Enciso Julia Medina Franco Mauricio Cesar Mauricio-Vilchez Daniel Alvitez-Temoche Luzmila Vilchez Frank Mayta-Tovalino Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study International Journal of Dentistry |
| title | Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study |
| title_full | Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study |
| title_fullStr | Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study |
| title_full_unstemmed | Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study |
| title_short | Antibacterial Effectiveness of Four Concentrations of the Hydroalcoholic Extract of Solanum tuberosum (Tocosh) against Streptococcus mutans ATCC 25175TM: A Comparative In Vitro Study |
| title_sort | antibacterial effectiveness of four concentrations of the hydroalcoholic extract of solanum tuberosum tocosh against streptococcus mutans atcc 25175tm a comparative in vitro study |
| url | http://dx.doi.org/10.1155/2020/8856382 |
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