A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens
A cost-effective and efficacious influenza vaccine for use in commercial poultry farms would help protect against avian influenza outbreaks. Current influenza vaccines for poultry are expensive and subtype specific, and therefore there is an urgent need to develop a universal avian influenza vaccine...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2013/316926 |
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| author | Kaleb A. Reese Christopher Lupfer Rudd C. Johnson Georgi M. Mitev Valerie M. Mullen Bruce L. Geller Manoj Pastey |
| author_facet | Kaleb A. Reese Christopher Lupfer Rudd C. Johnson Georgi M. Mitev Valerie M. Mullen Bruce L. Geller Manoj Pastey |
| author_sort | Kaleb A. Reese |
| collection | DOAJ |
| description | A cost-effective and efficacious influenza vaccine for use in commercial poultry farms would help protect against avian influenza outbreaks. Current influenza vaccines for poultry are expensive and subtype specific, and therefore there is an urgent need to develop a universal avian influenza vaccine. We have constructed a live bacterial vaccine against avian influenza by expressing a conserved peptide from the ectodomain of M2 antigen (M2e) on the surface of Lactococcus lactis (LL). Chickens were vaccinated intranasally with the lactococcal vaccine (LL-M2e) or subcutaneously with keyhole-limpet-hemocyanin conjugated M2e (KLH-M2e). Vaccinated and nonvaccinated birds were challenged with high pathogenic avian influenza virus A subtype H5N2. Birds vaccinated with LL-M2e or KLH-M2e had median survival times of 5.5 and 6.0 days, respectively, which were significantly longer than non-vaccinated birds (3.5 days). Birds vaccinated subcutaneously with KLH-M2e had a lower mean viral burden than either of the other two groups. However, there was a significant correlation between the time of survival and M2e-specific serum IgG. The results of these trials show that birds in both vaccinated groups had significantly (P<0.05) higher median survival times than non-vaccinated birds and that this protection could be due to M2e-specific serum IgG. |
| format | Article |
| id | doaj-art-925f7c44f57e4ab180acdf466ed01905 |
| institution | Kabale University |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-925f7c44f57e4ab180acdf466ed019052025-02-03T05:46:09ZengWileyVeterinary Medicine International2090-81132042-00482013-01-01201310.1155/2013/316926316926A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated ChickensKaleb A. Reese0Christopher Lupfer1Rudd C. Johnson2Georgi M. Mitev3Valerie M. Mullen4Bruce L. Geller5Manoj Pastey6Department of Microbiology, Oregon State University, 226 Nash Hall, Corvallis, OR 97331-3804, USADepartment of Biomedical Sciences, Oregon State University, 210 Dryden Hall, Corvallis, OR 97331-4801, USADepartment of Microbiology, Oregon State University, 226 Nash Hall, Corvallis, OR 97331-3804, USADepartment of Microbiology, Oregon State University, 226 Nash Hall, Corvallis, OR 97331-3804, USADepartment of Microbiology, Oregon State University, 226 Nash Hall, Corvallis, OR 97331-3804, USADepartment of Microbiology, Oregon State University, 226 Nash Hall, Corvallis, OR 97331-3804, USADepartment of Biomedical Sciences, Oregon State University, 210 Dryden Hall, Corvallis, OR 97331-4801, USAA cost-effective and efficacious influenza vaccine for use in commercial poultry farms would help protect against avian influenza outbreaks. Current influenza vaccines for poultry are expensive and subtype specific, and therefore there is an urgent need to develop a universal avian influenza vaccine. We have constructed a live bacterial vaccine against avian influenza by expressing a conserved peptide from the ectodomain of M2 antigen (M2e) on the surface of Lactococcus lactis (LL). Chickens were vaccinated intranasally with the lactococcal vaccine (LL-M2e) or subcutaneously with keyhole-limpet-hemocyanin conjugated M2e (KLH-M2e). Vaccinated and nonvaccinated birds were challenged with high pathogenic avian influenza virus A subtype H5N2. Birds vaccinated with LL-M2e or KLH-M2e had median survival times of 5.5 and 6.0 days, respectively, which were significantly longer than non-vaccinated birds (3.5 days). Birds vaccinated subcutaneously with KLH-M2e had a lower mean viral burden than either of the other two groups. However, there was a significant correlation between the time of survival and M2e-specific serum IgG. The results of these trials show that birds in both vaccinated groups had significantly (P<0.05) higher median survival times than non-vaccinated birds and that this protection could be due to M2e-specific serum IgG.http://dx.doi.org/10.1155/2013/316926 |
| spellingShingle | Kaleb A. Reese Christopher Lupfer Rudd C. Johnson Georgi M. Mitev Valerie M. Mullen Bruce L. Geller Manoj Pastey A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens Veterinary Medicine International |
| title | A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens |
| title_full | A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens |
| title_fullStr | A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens |
| title_full_unstemmed | A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens |
| title_short | A Novel Lactococcal Vaccine Expressing a Peptide from the M2 Antigen of H5N2 Highly Pathogenic Avian Influenza A Virus Prolongs Survival of Vaccinated Chickens |
| title_sort | novel lactococcal vaccine expressing a peptide from the m2 antigen of h5n2 highly pathogenic avian influenza a virus prolongs survival of vaccinated chickens |
| url | http://dx.doi.org/10.1155/2013/316926 |
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