Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker
Rifampicin-resistant marker was employed as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. V. harveyi M. G3 and G7 that difference not schizotyping as shown by Pulsed-Filed Gel Electrophoresis (PFGE) used in this study. Spontaneous mutation was conducted to generate V. harv...
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2007-12-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/4070 |
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| author | . Widanarni D. Meha Sri Nuryati . Sukenda A. Suwanto |
| author_facet | . Widanarni D. Meha Sri Nuryati . Sukenda A. Suwanto |
| author_sort | . Widanarni |
| collection | DOAJ |
| description |
Rifampicin-resistant marker was employed as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. V. harveyi M. G3 and G7 that difference not schizotyping as shown by Pulsed-Filed Gel Electrophoresis (PFGE) used in this study. Spontaneous mutation was conducted to generate V. harveyi resistant to rifampicin. Two groups of shrimp post-larvae (PL5) were immersed for 30 min in 106 CFU/ml of mutants and wild type of V. harveyi, respectively; and then placed in a 2 liter shrimp rearing tank for five days. A control group was immersed in sterile seawater. Growth curve analysis and pathogenicity assay of V. harveyi showed that each of the V. harveyi mutant exhibited almost identical profiles to that of the wild type parental strain and did not show alteration in their pathogenicity. Sample from dead shrimp larvae showed that the dead shrimp larvae were infected by V. harveyi RfR, indicated that rifampicin-resistant marker effective as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae.
Key words: shrimp larvae, Vibrio harveyi, rifampicin-resistant, molecular marker
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| format | Article |
| id | doaj-art-924bdf68a926480c86e37846b8f2cd73 |
| institution | Kabale University |
| issn | 1412-5269 2354-6700 |
| language | English |
| publishDate | 2007-12-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj-art-924bdf68a926480c86e37846b8f2cd732025-01-24T18:02:51ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002007-12-013310.19027/jai.3.23-273678Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker. Widanarni0D. Meha1Sri Nuryati2. Sukenda3A. Suwanto4Bogor Agricultural University, Department AquacultureDepartemen Budidaya Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian BogorBogor Agricultural University, Department AquacultureBogor Agricultural Institute, Department AquacultureDepartemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam. Institut Pertanian Bogor Rifampicin-resistant marker was employed as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. V. harveyi M. G3 and G7 that difference not schizotyping as shown by Pulsed-Filed Gel Electrophoresis (PFGE) used in this study. Spontaneous mutation was conducted to generate V. harveyi resistant to rifampicin. Two groups of shrimp post-larvae (PL5) were immersed for 30 min in 106 CFU/ml of mutants and wild type of V. harveyi, respectively; and then placed in a 2 liter shrimp rearing tank for five days. A control group was immersed in sterile seawater. Growth curve analysis and pathogenicity assay of V. harveyi showed that each of the V. harveyi mutant exhibited almost identical profiles to that of the wild type parental strain and did not show alteration in their pathogenicity. Sample from dead shrimp larvae showed that the dead shrimp larvae were infected by V. harveyi RfR, indicated that rifampicin-resistant marker effective as a reporter to assay pathogenicity of Vibrio harveyi in shrimp larvae. Key words: shrimp larvae, Vibrio harveyi, rifampicin-resistant, molecular marker https://journal.ipb.ac.id/index.php/jai/article/view/4070 |
| spellingShingle | . Widanarni D. Meha Sri Nuryati . Sukenda A. Suwanto Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker Jurnal Akuakultur Indonesia |
| title | Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker |
| title_full | Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker |
| title_fullStr | Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker |
| title_full_unstemmed | Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker |
| title_short | Pathogenicity Assay of Vibrio harveyi in Tiger Shrimp Larvae Employing Rifampicin-Resistant as A Molecular Marker |
| title_sort | pathogenicity assay of vibrio harveyi in tiger shrimp larvae employing rifampicin resistant as a molecular marker |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/4070 |
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