GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES
The paper presents an overview of studies of genetic polymorphism of loci determining bread-making quality among Ukrainian wheat varieties by means of electrophoretic analysis of storage proteins (Gli and Glu) and PCR. The results of analysis of polymorphism in the Gli-A1, Gli-B1, Gli-D1, Glu-A3 lo...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2014-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/27 |
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| author | S. V. Chebotar E. M. Blagodarova E. A. Kurakina I. V. Semenyuk A. M. Polishchuk N. A. Kozub I. A. Sozinov A. N. Khokhlov A. I. Ribalka Yu. M. Sivolap |
| author_facet | S. V. Chebotar E. M. Blagodarova E. A. Kurakina I. V. Semenyuk A. M. Polishchuk N. A. Kozub I. A. Sozinov A. N. Khokhlov A. I. Ribalka Yu. M. Sivolap |
| author_sort | S. V. Chebotar |
| collection | DOAJ |
| description | The paper presents an overview of studies of genetic polymorphism of loci determining bread-making quality among Ukrainian wheat varieties by means of electrophoretic analysis of storage proteins (Gli and Glu) and PCR. The results of analysis of polymorphism in the Gli-A1, Gli-B1, Gli-D1, Glu-A3 loci, as well as the allelic compositions of the Pina-D1 and Pinb-D1 genes, determining grain hardness, and Wx genes, controlling amylose content in the starch of the endosperm, are presented. Comparison of the results obtained with allele-specific primers to the γ gliadin loci and by storage protein electrophoresis shows that the former method can identify some alleles, but the level of polymorphism revealed by this method is lower than that revealed by electrophoresis of storage proteins. Most of the varieties under consideration have PCR alleles characteristic for hard wheat, and only three varieties have alleles specific for soft wheat. Varieties originating from different Ukrainian breeding-centers differ in grain hardness. PCR analysis has not revealed the presence of null alleles of Wx genes in the studied Ukrainian genetic pool but allowed selection of breeding material for further development of low-amylose varieties. |
| format | Article |
| id | doaj-art-924a625e33d34491904fe0f47d946083 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2014-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-924a625e33d34491904fe0f47d9460832025-02-01T09:57:58ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-01161879811GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIESS. V. Chebotar0E. M. Blagodarova1E. A. Kurakina2I. V. Semenyuk3A. M. Polishchuk4N. A. Kozub5I. A. Sozinov6A. N. Khokhlov7A. I. Ribalka8Yu. M. Sivolap9South Plant Biotechnology Center, National Academy of Agrarian Sciences of Ukraine, OdessaPlant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation, OdessaOdessa National State University, Department of Genetics and Molecular Biology, OdessaSouth Plant Biotechnology Center, National Academy of Agrarian Sciences of Ukraine, OdessaSouth Plant Biotechnology Center, National Academy of Agrarian Sciences of Ukraine, OdessaInstitute of Plant Protection UAAS, KievInstitute of Plant Protection UAAS, KievPlant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation, OdessaPlant Breeding and Genetics Institute – National Center of Seed and Cultivar Investigation, OdessaSouth Plant Biotechnology Center, National Academy of Agrarian Sciences of Ukraine, OdessaThe paper presents an overview of studies of genetic polymorphism of loci determining bread-making quality among Ukrainian wheat varieties by means of electrophoretic analysis of storage proteins (Gli and Glu) and PCR. The results of analysis of polymorphism in the Gli-A1, Gli-B1, Gli-D1, Glu-A3 loci, as well as the allelic compositions of the Pina-D1 and Pinb-D1 genes, determining grain hardness, and Wx genes, controlling amylose content in the starch of the endosperm, are presented. Comparison of the results obtained with allele-specific primers to the γ gliadin loci and by storage protein electrophoresis shows that the former method can identify some alleles, but the level of polymorphism revealed by this method is lower than that revealed by electrophoresis of storage proteins. Most of the varieties under consideration have PCR alleles characteristic for hard wheat, and only three varieties have alleles specific for soft wheat. Varieties originating from different Ukrainian breeding-centers differ in grain hardness. PCR analysis has not revealed the presence of null alleles of Wx genes in the studied Ukrainian genetic pool but allowed selection of breeding material for further development of low-amylose varieties.https://vavilov.elpub.ru/jour/article/view/27bread wheatpcrelectrophoresisgliadinsgluteninsgrain hardnesspuroindoline geneswx genes |
| spellingShingle | S. V. Chebotar E. M. Blagodarova E. A. Kurakina I. V. Semenyuk A. M. Polishchuk N. A. Kozub I. A. Sozinov A. N. Khokhlov A. I. Ribalka Yu. M. Sivolap GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES Вавиловский журнал генетики и селекции bread wheat pcr electrophoresis gliadins glutenins grain hardness puroindoline genes wx genes |
| title | GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES |
| title_full | GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES |
| title_fullStr | GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES |
| title_full_unstemmed | GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES |
| title_short | GENETIC POLYMORPHISM OF LOCI DETERMINING BREAD MAKING QUALITY IN UKRAINIAN WHEAT VARIETIES |
| title_sort | genetic polymorphism of loci determining bread making quality in ukrainian wheat varieties |
| topic | bread wheat pcr electrophoresis gliadins glutenins grain hardness puroindoline genes wx genes |
| url | https://vavilov.elpub.ru/jour/article/view/27 |
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