Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study

Abstract Essential trace elements, such as iron (Fe) and selenium (Se), play physiological roles in our body, whereas environmental toxic metals, such as arsenic (As), cadmium (Cd), and mercury (Hg), are known to be associated with various disease risks. However, the relationship between elements, b...

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Main Authors: Yoshiro Saito, Misaki Shimizu, Mitsuharu Sato, An Masuda, Kotoko Arisawa, Keiko Taguchi, Takashi Toyama, Ikuko N. Motoike, Kengo Kinoshita, Seizo Koshiba, Masayuki Yamamoto, Toshinari Takamura
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-10581-2
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author Yoshiro Saito
Misaki Shimizu
Mitsuharu Sato
An Masuda
Kotoko Arisawa
Keiko Taguchi
Takashi Toyama
Ikuko N. Motoike
Kengo Kinoshita
Seizo Koshiba
Masayuki Yamamoto
Toshinari Takamura
author_facet Yoshiro Saito
Misaki Shimizu
Mitsuharu Sato
An Masuda
Kotoko Arisawa
Keiko Taguchi
Takashi Toyama
Ikuko N. Motoike
Kengo Kinoshita
Seizo Koshiba
Masayuki Yamamoto
Toshinari Takamura
author_sort Yoshiro Saito
collection DOAJ
description Abstract Essential trace elements, such as iron (Fe) and selenium (Se), play physiological roles in our body, whereas environmental toxic metals, such as arsenic (As), cadmium (Cd), and mercury (Hg), are known to be associated with various disease risks. However, the relationship between elements, biochemical parameters, and lifestyle habits based on multi-elemental analysis in healthy individuals has not been fully verified. Multi-elemental analysis is useful for evaluating the change in the concentration of these elements and metals. In the present study using totally 100 µL plasma samples from the Tohoku Medical Megabank (TMM) community-based cohort study (total of 506 specimens), we conducted a multi-elemental analysis to evaluate 14 elements in generally healthy subjects. We further determined Se-transporter selenoprotein P levels using the originally developed ELISA method, since increases and decreases in selenoprotein P levels are associated with various disease risks. Multiple correlation analyses between the obtained measured values and several factors suggest that elements such as Fe, Se, and Hg, as well as selenoprotein P levels, are associated with gender differences. We also found that factors such as Fe, Se, As, Hg, hematocrit value, hemoglobin (Hb) content, and HbA1c are correlated with selenoprotein P levels. Furthermore, correlations between Fe levels and Hb content and between As/Hg and fish consumption were found. These findings demonstrate the suitability of multi-elemental analyses with limited plasma sample amounts, clearly show gender-differentiated elements, and establish a significant relationship between selenoprotein P and Fe metabolism.
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spelling doaj-art-9219a4b3d87e47a78881f3d0a2e3fda92025-08-20T03:43:21ZengNature PortfolioScientific Reports2045-23222025-07-0115111510.1038/s41598-025-10581-2Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort studyYoshiro Saito0Misaki Shimizu1Mitsuharu Sato2An Masuda3Kotoko Arisawa4Keiko Taguchi5Takashi Toyama6Ikuko N. Motoike7Kengo Kinoshita8Seizo Koshiba9Masayuki Yamamoto10Toshinari Takamura11Laboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku UniversityLaboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku UniversityTohoku Medical Megabank Organization, Tohoku UniversityLaboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku UniversityLaboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku UniversityTohoku Medical Megabank Organization, Tohoku UniversityLaboratory of Molecular Biology and Metabolism, Graduate School of Pharmaceutical Sciences, Tohoku UniversityTohoku Medical Megabank Organization, Tohoku UniversityTohoku Medical Megabank Organization, Tohoku UniversityTohoku Medical Megabank Organization, Tohoku UniversityTohoku Medical Megabank Organization, Tohoku UniversityDepartment of Endocrinology and Metabolism, Kanazawa University Graduate School of Medical SciencesAbstract Essential trace elements, such as iron (Fe) and selenium (Se), play physiological roles in our body, whereas environmental toxic metals, such as arsenic (As), cadmium (Cd), and mercury (Hg), are known to be associated with various disease risks. However, the relationship between elements, biochemical parameters, and lifestyle habits based on multi-elemental analysis in healthy individuals has not been fully verified. Multi-elemental analysis is useful for evaluating the change in the concentration of these elements and metals. In the present study using totally 100 µL plasma samples from the Tohoku Medical Megabank (TMM) community-based cohort study (total of 506 specimens), we conducted a multi-elemental analysis to evaluate 14 elements in generally healthy subjects. We further determined Se-transporter selenoprotein P levels using the originally developed ELISA method, since increases and decreases in selenoprotein P levels are associated with various disease risks. Multiple correlation analyses between the obtained measured values and several factors suggest that elements such as Fe, Se, and Hg, as well as selenoprotein P levels, are associated with gender differences. We also found that factors such as Fe, Se, As, Hg, hematocrit value, hemoglobin (Hb) content, and HbA1c are correlated with selenoprotein P levels. Furthermore, correlations between Fe levels and Hb content and between As/Hg and fish consumption were found. These findings demonstrate the suitability of multi-elemental analyses with limited plasma sample amounts, clearly show gender-differentiated elements, and establish a significant relationship between selenoprotein P and Fe metabolism.https://doi.org/10.1038/s41598-025-10581-2Multi-elemental analysisEssential trace elementsHeavy metalsSelenoprotein PTohoku Medical MegabankCommunity-based cohort study
spellingShingle Yoshiro Saito
Misaki Shimizu
Mitsuharu Sato
An Masuda
Kotoko Arisawa
Keiko Taguchi
Takashi Toyama
Ikuko N. Motoike
Kengo Kinoshita
Seizo Koshiba
Masayuki Yamamoto
Toshinari Takamura
Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study
Scientific Reports
Multi-elemental analysis
Essential trace elements
Heavy metals
Selenoprotein P
Tohoku Medical Megabank
Community-based cohort study
title Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study
title_full Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study
title_fullStr Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study
title_full_unstemmed Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study
title_short Gender differences in plasma element concentrations and associations between selenoprotein P and iron metabolism in a community-based cohort study
title_sort gender differences in plasma element concentrations and associations between selenoprotein p and iron metabolism in a community based cohort study
topic Multi-elemental analysis
Essential trace elements
Heavy metals
Selenoprotein P
Tohoku Medical Megabank
Community-based cohort study
url https://doi.org/10.1038/s41598-025-10581-2
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